Expression and purification of the recombinant membrane protein YidC: A case study for increased stability and solubility

Protein Expression and Purification

Volumn 62, Issue 1, 2008, Pages 49-52

  Martinez Molina, Daniel ^a,b   Lundbäck, Anna Karin ^a,c   Niegowski, Damian ^a,b   Eshaghi, Said ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

^c ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Analytical size exclusion chromatography; Buffer screen; Gel filtration; Membrane protein insertion system; Stability optimization

Indexed keywords

BUFFER; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN; YIDC PROTEIN, E COLI;

ARTICLE; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; SOLUBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BUFFERS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; MEMBRANE TRANSPORT PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 52949101714     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.05.011     Document Type: Article

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