Viability and function in lymphocytes cultured from the horse, chicken, and mouse: Effects of different leukocyte enrichment techniques

Journal of Immunoassay and Immunochemistry

Volumn 29, Issue 4, 2008, Pages 370-389

  Mustafa, A ^a   Gillmeister, L ^a   Hernandez, W P ^d   Larsen, C T ^b   Witonsky, S ^b   Holladay, S D ^a   Kerr, R P ^a   Ahmed, S A ^a   Santo, A ^c   Gogal Jr , R M ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

^c EDWARD VIA COLLEGE OF OSTEOPATHIC MEDICINE   (United States)

^d NAVAL HOSPITAL   (United States)

Author keywords

7 AAD; Chicken; Horse; Lymphocyte isolation; Mouse; Viability

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL FUNCTION; CELL PROLIFERATION; CELL VIABILITY; CHICKEN; EXPERIMENTAL ANIMAL; FEMALE; FOWL; HORSE; LEUKOCYTE; LYMPHOCYTE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; APOPTOSIS; CELL PROLIFERATION; CELL SEPARATION; CELL SURVIVAL; CELLS, CULTURED; CHICKENS; CONCANAVALIN A; FEMALE; HORSES; LYMPHOCYTE COUNT; LYMPHOCYTES; MALE; MICE;

EID: 52949101057     PISSN: 15321819     EISSN: 15324230     Source Type: Journal    
DOI: 10.1080/15321810802329823     Document Type: Article

References (19)

1. Klein, A.B.; Witonsky, S.G.; Ahmed, S.A.; Holladay, S.D.; Gogal, Jr., R.M.; Link, L.; Reilly, C.M. Impact of different cell isolation techniques on lymphocyte viability and function. J Immunoassay Immunochem. 2006, 27 (1), 61-76.
2. Ruitenberg, J.J.; Mulder, C.B.; Maino, V.C.; Landay, A.L.; Ghanekar, S.A. VACUTAINER CPT and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples. BMC Immunol. 2006, 7, 11.
3. Tochizawa, S.; Ohmoto, Y.; Mori, T. A novel modification of a flow cytometric assay of phosphorylated STAT1 in whole blood lymphocytes for rapid detection of interferon-alpha signal in vivo. J Immunol. Meth. 2006, 313 (1-2), 29-37.
4. Richter, C.A.; Birnbaum, L.S.; Farabollini, F.; Newbold, R.R.; Rubin, B.S.; Talsness, C.E.; Vandenbergh, J.G.; Walser-Kuntz, D.R.; Vom Saal, F.S. In vivo effects of bisphenol A in laboratory rodent studies. Reprod. Toxicol. 2007, 24 (2): 199-224.
5. Green, E.L. (Ed.). Biology of the Laboratory Mouse; McGraw-Hill: New York, 1966.
6. Boyum, A.; Brincker Fjerdingstad, H.; Martinsen, I.; Lea, T.; Lovhaug, D. Separation of human lymphocytes from citrated blood by density gradient (NycoPrep) centrifugation: monocyte depletion depending upon activation of membrane potassium channels. Scand. J. Immunol. 2002, 56 (1), 76-84.
7. Witonsky, S.; Gogal, R.M., Jr.; Buechner-Maxwell, V.; Ahmed, S.A. Immunologic analysis of blood samples obtained from horses and stored for twenty-four hours. Am. J. Vet. Res. 2003, 64 (8), 1003-1009.
8. Ahmed, S.A.; Gogal, R.M., Jr.; Walsh, J.E. A new rapid and simple nonradioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J. Immunol. Meth. 1994, 170 (2), 211-224.
9. Gogal, Jr., R.M.; Ahmed, S.A.; Larsen, C.T. Analysis of avian lymphocyte proliferation by a new, simple, nonradioactive assay (lympho-pro). Avian Dis. 1997, 41 (3), 714-725.
10. Gogal, Jr., R.M.; Prater, M.R.; Smith, B.J.; Johnson, M.S.; Holladay, S.D. Bilateral dissected spleens and thymuses in rodents exhibit homogeneity in leukocyte markers. Toxicology. 2001, 157 (3), 217-223.
11. Donner, K.J.; Becker, K.M.; Hissong, B.D.; Ahmed, S.A. Comparison of multiple assays for kinetic detection of apoptosis in thymocytes exposed to dexamethasone or diethylstilbesterol. Cytometry. 1999, 35 (1), 80-90.
12. Gogal, Jr., R.M.; Ahmed, S.A.; Holladay, S.D.; Walsh, J.E.; Galaburda, A.M.; Rosen G.D. Induced minor malformations in the neocortex of normal mice do not alter immunological functions. Immunol. Invest. 2000, 29 (3), 299-318.
13. Witonsky, S.G.; Gogal, Jr., R.M.; Duncan, R.B.; Lindsay, D.S. Protective immune response to experimental infection with Sarcocystis neurona in 57BL/6 mice. J. Parasitol. 2003, 89 (5), 924-931.
14. Gogal, Jr., R.M.; Smith, B.J.; Kalnitsky, J.; Holladay, S.D. Analysis of apoptosis of lymphoid cells in fish exposed to immunotoxic compounds. Cytometry 2000, 39 (4), 310-318.
15. Bruyninckx, W.J.; Blancquaert, A.M. Isolation of horse mononuclear cells, especially of monocytes, on Isopaque-Ficoll neutral density gradient. Vet. Immunol. Immunopathol. 1983, 4 (4), 493-504.
16. Moore, T.; Wilcke, J.; Chilcoat, C.; Eyre, P.; Crisman, M. Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate ex vivo. Vet. Immunol. Immunopathol. 1997, 56 (3-4), 233-246.
17. Altrade;an, S.A.; Randers, L.; Rao, G. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnol. Prog. 1993, 9 (6), 671-674.
18. Schmid, I.; Uittenbogaart, C.H.; Keld, B.; Giorgi, J.V. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Meth. 1994, 170 (2), 145-157.
19. Kerr, J.F.; Wyllie, A.H.; Currie, A.R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Brit. J. Cancer. 1972, 26 (4), 239-257.