A species-specific PCR for Lactobacillus iners demonstrates a relative specificity of this species for vaginal colonization

Microbial Ecology in Health and Disease

Volumn 20, Issue 3, 2008, Pages 135-139

  Alqumber, Mohammed A ^a   Burton, Jeremy P ^a   Devenish, Celia ^a   Tagg, John R ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Lactobacillus iners; Species specific PCR; Vaginal colonization

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; FECES ANALYSIS; FEMALE; GENE SEQUENCE; HUMAN; LACTOBACILLUS; LACTOBACILLUS INERS; NONHUMAN; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA GENE; SALIVA ANALYSIS; SPECIES DIFFERENCE; VAGINA; VAGINA FLORA;

LACTOBACILLUS; LACTOBACILLUS INERS;

EID: 52949083566     PISSN: 0891060X     EISSN: 16512235     Source Type: Journal    
DOI: 10.1080/08910600802340967     Document Type: Article

References (23)

1. Fuller R. The importance of Lactobacilli in maintaining normal microbial balance in the crop. Br Poult Sci. 1977;18:85-94.
2. Watkins BA, Kratzer FH. Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks. Poult Sci. 1983;62:2088-94.
3. Watkins BA, Miller BF. Competitive gut exclusion of avian pathogens by Lactobacillus acidophilus in gnotobiotic chicks. Poult Sci. 1983;62:1772-9.
4. Reid G, Bruce AW, McGroarty JA, Cheng KJ, Costerton JW. Is there a role for lactobacilli in prevention of urogenital and intestinal infections? Clin Microbiol Rev. 1990;3:335-44.
5. Bruce AW, Reid G. Intravaginal instillation of lactobacilli for prevention of recurrent urinary tract infections. Can J Microbiol. 1988;34:339-43.
6. Reid G, Charbonneau D, Erb J, Kochanowski B, Beuerman D, Poehner R, et al. Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. FEMS Immunol Med Microbiol. 2003;35:131-4.
7. Falsen E, Pascual C, Sjoden B, Ohlen M, Collins MD. Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov. Int J Syst Bacteriol. 1999;49(Pt 1):217-21.
8. Dickson EM, Riggio MP, Macpherson L. A novel species-specific PCR assay for identifying Lactobacillus fermentum. J Med Microbiol. 2005;54(Pt 3):299-303.
9. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med. 2005;353:1899-911.
10. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, et al. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol. 2000;66:297-303.
11. Wilson KH, Blitchington RB, Greene RC. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol. 1990;28:1942-6.
12. Horie M, Kajikawa HS, Toba T. Identification of Lactobacillus crispatus by polymerase chain reaction targeting S-layer protein gene. Lett Appl Microbiol. 2002;35:57-61.
13. Burton JP, Reid G. Evaluation of the bacterial vaginal flora of 20 postmenopausal women by direct (Nugent score) and molecular (polymerase chain reaction and denaturing gradient gel electrophoresis) techniques. J Infect Dis. 2002;186:1770-80.
14. Zhou X, Bent SJ, Schneider MG, Davis CC, Islam MR, Forney LJ. Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods. Microbiology. 2004;150(Pt 8):2565-73.
15. Burton JP, Cadieux PA, Reid G. Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. Appl Environ Microbiol. 2003;69:97-101.
16. Tarnberg M, Jakobsson T, Jonasson J, Forsum U. Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions. APMIS. 2002;110:802-10.
17. Vasquez A, Jakobsson T, Ahrne S, Forsum U, Molin G. Vaginal lactobacillus flora of healthy Swedish women. J Clin Microbiol. 2002;40:2746-9.
18. Hayashi H, Sakamoto M, Benno Y. Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods. Microbiol Immunol. 2002;46:535-48.
19. Sakamoto M, Umeda M, Benno Y. Molecular analysis of human oral microbiota. J Periodontal Res. 2005;40:277-85.
20. Diaz PI, Chalmers NI, Rickard AH, Kong C, Milburn CL, Palmer RJ Jr, et al. Molecular characterization of subject-specific oral microflora during initial colonization of enamel. Appl Environ Microbiol. 2006;72:2837-48.
21. de Lillo A, Ashley FP, Palmer RM, Munson MA, Kyriacou L, Weightman AJ, et al. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol Immunol. 2006;21:61-8.
22. Antonio MA, Rabe LK, Hillier SL. Colonization of the rectum by Lactobacillus species and decreased risk of bacterial vaginosis. J Infect Dis. 2005;192:394-8.
23. Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP. Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2001;67:2578-85 .