Steady-state kinetic analysis of human ubiquitin-activating enzyme (E1) using a fluorescently labeled ubiquitin substrate

Journal of Protein Chemistry

Volumn 19, Issue 6, 2000, Pages 489-498

  Wee, Kevin E ^a   Lai, Zhihong ^a   Auger, Kurt R ^a   Ma, Jianhong ^a   Horiuchi, Kurumi Y ^a   Dowling, Randine L ^a   Dougherty, Cristy S ^a   Corman, Jeanne I ^a   Wynn, Richard ^a   Copeland, Robert A ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Activity assay; Fluorescence; Human E1; Steady state kinetics; Ubiquitin; Ubiquitin activating enzyme

Indexed keywords

UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; CATALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBSTRATE; STEADY STATE;

BINDING, COMPETITIVE; FLUORESCEINS; HUMANS; KINETICS; LIGASES; MODELS, MOLECULAR; PROTEIN STRUCTURE, QUATERNARY; SUBSTRATE SPECIFICITY; UBIQUITIN-ACTIVATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINS; ULTRACENTRIFUGATION;

EID: 52849137566     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026501515450     Document Type: Article

References (22)