Construction of arsB and tetH mutants of the sulfur-oxidizing bacterium Acidithiobacillus caldus by marker exchange

Applied and Environmental Microbiology

Volumn 74, Issue 18, 2008, Pages 5686-5694

  Van Zyl, Leonardo J ^a   Van Munster, Jolanda M ^a   Rawlings, Douglas E ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ARSENIC; ARSENIC COMPOUNDS; CELL GROWTH; ESCHERICHIA COLI; EXTRACTION; GOLD; GOLD DEPOSITS; GROWTH KINETICS; HEALTH; METAL RECOVERY; METALS; MINERALS; NONMETALS; ORES; SULFUR;

ACIDITHIOBACILLUS CALDUS; CONJUGATION SYSTEMS; DUAL USE; GENE INACTIVATION; HYBRIDIZATION PROBE; KANAMYCIN RESISTANCE; MODERATELY THERMOPHILIC; PLASMID VECTORS; SULFUR-OXIDIZING BACTERIUM; TETRATHIONATE;

ORE TREATMENT;

ARSENIC; ARSENOPYRITE; CHROMOSOME; EXTRACTION; GENETIC MARKER; GROWTH RESPONSE; HOST RANGE; HYBRIDIZATION; MUTATION; OXIDATION; PHYSIOLOGY; PLASMID; SULFUR; THERMOPHILIC BACTERIUM;

ACIDITHIOBACILLUS; ARTICLE; BACTERIUM CONJUGATION; BACTERIUM MUTANT; CELL GROWTH; DNA HYBRIDIZATION; GENE DELETION; GENE INACTIVATION; NONHUMAN; OXIDATION; SULFUR OXIDIZING BACTERIUM; BACTERIAL CHROMOSOME; BACTERIAL GENE; DRUG EFFECT; ESCHERICHIA COLI; GENE TARGETING; GENE VECTOR; GENETIC MARKER; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MUTAGENESIS; PLASMID;

ACIDITHIOBACILLUS CALDUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ARSENIC TRIOXIDE; ARSENOUS ACID DERIVATIVE; BACTERIAL DNA; TETRATHIONIC ACID;

ACIDITHIOBACILLUS; ARSENITES; CHROMOSOMES, BACTERIAL; CONJUGATION, GENETIC; DNA, BACTERIAL; ESCHERICHIA COLI; GENE TARGETING; GENES, BACTERIAL; GENETIC MARKERS; GENETIC VECTORS; MUTAGENESIS; PLASMIDS; TETRATHIONIC ACID;

EID: 52649163967     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01235-08     Document Type: Article

References (32)

1. Biswas, I., A. Gruss, D. S. Ehrlich, and E. Maguin. 1993. High-efficiency gene inactivation and replacement system for gram-positive bacteria. J. Bacteriol. 175:3628-3635.
2. Bugaytsova, Z., and E. B. Lindström. 2004. Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus. Eur. J. Biochem. 271:272-280.
3. Datta, N., and R. W. Hedges. 1972. Trimethoprim resistance conferred by W plasmid in Enterobacteriaceae. J. Gen. Microbiol. 72:349-355.
4. Dopson, M., and E. B. Lindström. 1999. Potential role of Thiobacillus caldus in arsenopyrite bioleaching. Appl. Environ. Microbiol. 65:36-40.
5. Dopson, M., and E. B. Lindström. 2004. Analysis of community composition during moderately thermophilic leaching of pyrite, arsenical pyrite and chalcopyrite. Microb. Ecol. 48:19-28.
6. Dopson, M., E. B. Lindström, and K. B. Hallberg. 2002. ATP generation during reduced inorganic sulfur compound oxidation by Acidithiobacillus caldus due to electron transport phosphorylation. Extremophiles 6:123-129.
7. Gardner, M. N., S. M. Deane, and D. E. Rawlings. 2001. Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon. J. Bacteriol. 183:3303-3309.
8. Hallberg, K. B., and E. B. Lindström. 1994. Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile. Microbiology 140: 3451-3456.
9. Hallberg, K. B., M. Dopson, and E. B. Lindström. 1996. Reduced sulfur compound oxidation by Thiobacillus caldus. J. Bacteriol. 178:6-11.
10. Hamilton, C. M., M. Aldea, B. K. Washburn, P. Babitzke, and S. R. Kushner. 1989. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-4622.
11. Hasty, P., J. Rivera-Pérez, and A. Bradley. 1991. The length of homology required for gene targeting in embryonic stem cells. Mol. Cell. Biol. 11:5586-5591.
12. Hawkes, R. B., P. D. Franzmann, and J. J. Plumb. 2006. Moderate thermophiles including "Ferroplasma cyprexacervatum" sp. nov., dominate an industrial scale chalcocite heap bioleaching operation. Hydrometallurgy 83:229-236.
13. Jin, S. M., W. M. Yan, and Z. N. Wang. 1992. Transfer of IncP plasmids to extremely acidophilic Thiobacillus thiooxidans. Appl. Environ. Microbiol. 58:429-430.
14. Kelly, D. P., and A. P. Wood. 2000. Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov. Int. J. Syst. Evol. Microbiol. 50:511-516.
15. Kotze, A. A., M. I. Tuffin, S. M. Deane, and D. E. Rawlings. 2006. Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs. Microbiology 152:3551-3560.
16. Kusano, T., K. Sugawara, C. Inoue, T. Takeshima, M. Numata, and T. Shiratori. 1992. Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant. J. Bacteriol. 174:6617-6623.
17. Lacks, S., and B. Greenberg. 1977. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J. Mol. Biol. 114:153-168.
18. Liu, X., J. Lin, Z. Zhang, J. Bian, Q. Zhao, Y. Liu, J. Lin, and W. Yan. 2007. Construction of conjugative gene transfer system between E. coli and moderately thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. J. Microbiol. Biotechnol. 17:162-167.
19. Liu, Z., N. Guiliani, C. Appia-Ayme, F. Borne, J. Ratouchniak, and V. Bonnefoy. 2000. Construction and characterization of a recA mutant of Thiobacillus ferrooxidans by marker exchange mutagenesis. J. Bacteriol. 182:2269-2276.
20. Okibe, N., M. Gericke, K. B. Hallberg, and D. B. Johnson. 2003. Enumeration and characterization of acidophilic microorganisms isolated from a pilot plant stirred-tank bioleaching operation. Appl. Environ. Microbiol. 69:1936-1943.
21. Parish, T., and N. G. Stoker. 2000. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Microbiology 146:1969-1975.
22. Peng, J.-B., W.-M. Yan, and X.-Z. Bao. 1994. Plasmid and transposon transfer to Thiobacillus ferrooxidans. J. Bacteriol. 176:2892-2897.
23. Peng, J.-B., W.-M. Yan, and X.-Z. Bao. 1994. Expression of heterogenous arsenic resistance genes in the obligately autotrophic biomining bacterium Thiobacillus ferrooxidans. Appl. Environ. Microbiol. 60:2653-2656.
24. Rawlings, D. E., N. J. Coram, M. N. Gardner, and S. M. Deane. 1999. Thiobacillus caldus and Leptospirillum ferrooxidans are widely distributed in continuous flow biooxidation tanks used to treat a variety of metal containing ores and concentrates, p. 777-786. In R. Amils and A. Ballester (ed.), Biohydrometallurgy and the environment toward the mining of the 21st century, part A. Elsevier, Amsterdam, The Netherlands.
25. Rawlings, D. E., and D. B. Johnson. 2007. The microbiology of biomining: development and optimization of mineral-oxidizing microbial consortia. Microbiology 153:315-324.
26. Rzhepishevska, O. I., J. Valdés, L. Marcinkeviciene, C. A. Gallardo, R. Meskys, V. Bonnefoy, D. S. Holmes, and M. Dopson. 2007. Regulation of a novel Acidithiobacillus caldus gene cluster involved in metabolism of reduced inorganic sulfur compounds. Appl. Environ. Microbiol. 73:7367-7372.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
28. Silver, S., K. Budd, K. M. Leahy, W. V. Shaw, D. Hammond, R. P. Novick, J. R. Willsky, M. H. Malamy, and H. Rosenburg. 1981. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony(III) in Escherichia coli and Staphylococcus aureus. J. Bacteriol. 172:424-430.
29. Silverman, M. P., and D. G. Lundgren. 1958. Studies on the chemoautotrophic iron bacterium Ferrobacterium ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J. Bacteriol. 77:642-647.
30. Tian, K. L., J. Q. Lin, X. M. Liu, Y. Liu, C. K. Zhang, and W. M. Yan. 2003. Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans. Biotechnol. Lett. 25:749-754.
31. Yannisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
32. Zhao, Q., X. M. Liu, Y. Zhan, J. Q. Lin, W. M. Yan, J. Bian, and Y. Liu. 2005. Construction of an engineered Acidithiobacillus caldus with high-efficiency arsenic resistance. Wei Sheng Wu Xue Bao 45:675-679.