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1
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Searching sequence space
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DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
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Stemmer, W. P. C. (1994) DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA 91: 10747-10751.
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Rapid evolution of a protein in vitro by DNA shuffling
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Stemmer, W. P. C. (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-391.
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Nature
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Stemmer, W.P.C.1
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Improved green fluorescent protein by molecular evolution using DNA shuffling
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Crameri, A., E. Whitehorn, E. Tate, and W. P. C. Stemmer (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnol. 14: 315-319.
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Crameri, A.1
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5
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0030989062
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Evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening
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Zhang, J., G. Dawes, and W. P. C. Stemmer (1997) Evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening. Proc. Natl. Acad. Sci. USA 94: 4504-4509.
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0030951186
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Molecular evolution of an arsenate detoxification pathway by DNA shuffling
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Crameri, A., G. Dawes, E. Rodriguez, S. Silver, and W. P. C. Stemmer (1997) Molecular evolution of an arsenate detoxification pathway by DNA shuffling. Nature Biotechnol. 15: 436-438.
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Nature Biotechnol.
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Crameri, A.1
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7
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0032518266
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DNA shuffling of genes from diverse species accelerates directed evolution
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Crameri, A., S.-A. Raillard, E. Bermudez, and W. P. C. Stemmer (1998) DNA shuffling of genes from diverse species accelerates directed evolution. Nature 391: 288-291.
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Nature
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Crameri, A.1
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8
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0028784138
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Single-step PCR assembly of a gene and a whole plasmid from large numbers of oligonucleotides
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Stemmer, W.P.C., A. Crameri, K. D. Ha, T. M. Brennan, and H. L. Heyneker (1995) Single-step PCR assembly of a gene and a whole plasmid from large numbers of oligonucleotides. Gene 164: 49-53.
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Heyneker, H.L.5
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9
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0033037012
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Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling
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Christians, F C., L. Scapozza, A. Crameri, G. Folkers, and W. P. C. Stemmer (1999) Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nature Biotechnol. 17: 259-264.
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Christians, F.C.1
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0032885231
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Creation of a functionally diverse enzyme library by DNA family shuffling
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Ness, J., M. Welch, L. Giver, M. Bueno, J. Cherry, T. Borchert, W. P. C. Stemmer, and J. Minshull (1999) Creation of a functionally diverse enzyme library by DNA family shuffling. Nature Biotechnol. 17: 893-896.
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11
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0032866310
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Rapid evolution of a cytokine using molecular breeding
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Chang, C.-C., T. T. Chen, B. W. Cox, G. N. Dawes, W. P. C. Stemmer, J. Punnonen, and P. A. Patten (1999) Rapid evolution of a cytokine using molecular breeding. Nature Biotechnol. 17: 793-797.
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12
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0034426015
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Mole-cular breeding of viruses
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Soong, N.-W., L. Nomura, K. Pekrun, M. Reed, L. Sheppard, G. Dawes, and W. P. C. Stemmer (2000) Mole-cular breeding of viruses. Nature Genetics 25: 436-439.
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Nature Genetics
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Soong, N.-W.1
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Breeding of retroviruses by DNA shuffling for improved stability and processing yields
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Powell, S. K., M. A. Kaloss, A. Pinkstaff, R. McKee, I. Burimski, M. Pensiero, E. Otto, W. P. C. Stemmer, and N.-W. Soong (2000) Breeding of retroviruses by DNA shuffling for improved stability and processing yields. Nature Biotechnol. 18: 1279-1282.
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Molecular breeding of viruses for targeting and other clinical properties
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Stemmer, W. P. C. and N.-W. Soong (1999) Molecular breeding of viruses for targeting and other clinical properties. Tumor Targeting 4: 59-62.
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Stemmer, W.P.C.1
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0037034007
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Genome shuffling leads to rapid phenotypic improvement in bacteria
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Zhang, Y.-X., K. Perry, V. A. Vinci, K. Powell, W. P. C. Stemmer, and S. B. del Cardayre (2002) Genome shuffling leads to rapid phenotypic improvement in bacteria. Nature 415: 644-646.
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Nature
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Zhang, Y.-X.1
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52649089983
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Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes
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Raillard, S., A. Krebber, Y. Chen, J. E. Ness, E. Bermudez, R. Trinidad, R. Fullem, C. Davis, M. Welch, J. Seffernick, L. P. Wackett, W. P. C. Stemmer, and J. Minshull (2001) Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes. Chemistry Biol. 125: 1-9.
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