Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP

Microbiology and Immunology

Volumn 52, Issue 6, 2008, Pages 310-313

  Shima, Kensuke ^a   Kawamura, Naohisa ^b   Hinenoya, Atsushi ^a   Sugimoto, Norihiko ^a   Wu, Yuluo ^a   Asakura, Masahiro ^a   Nishimura, Kazuhiko ^a   Nair, Gopinath B ^c,d   Yamasaki, Shinji ^a  

^a Osaka Prefecture University   (Japan)

^b OSAKA ROSAI HOSPITAL   (Japan)

^c INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH   (Bangladesh)

^d NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES   (India)

Author keywords

Hemolytic uremic syndrome; Restriction fragment length polymorphisms; Shiga toxin producing Escherichia coli

Indexed keywords

CEFACLOR; FOSFOMYCIN; LINCOMYCIN; SHIGA TOXIN; BACTERIAL DNA;

ADOLESCENT; ARTICLE; BACTERIUM ISOLATION; CONTROLLED STUDY; DIARRHEA; DNA EXTRACTION; ESCHERICHIA COLI INFECTION; ESCHERICHIA COLI O157; FECES ANALYSIS; FEMALE; FEVER; GENETIC VARIABILITY; HEMATOCRIT; HUMAN; LACTOBACILLUS; MOLECULAR TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; BACTERIUM IDENTIFICATION; CLASSIFICATION; DIFFERENTIAL DIAGNOSIS; ENTEROBACTER INFECTION; FEASIBILITY STUDY; FECES; GENETICS; HEMOLYTIC UREMIC SYNDROME; ISOLATION AND PURIFICATION; MICROBIOLOGY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

ESCHERICHIA COLI;

BACTERIAL TYPING TECHNIQUES; DIAGNOSIS, DIFFERENTIAL; DIARRHEA; DNA, BACTERIAL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI O157; FEASIBILITY STUDIES; FECES; HEMOLYTIC-UREMIC SYNDROME; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 52649104433     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2008.00038.x     Document Type: Article

References (13)

1. Tarr P.I., Gordon C.A., Chandler,W.L. (2005) Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet 365: 1073-1086.
2. Bell B.P., Goldoft M., Griffin P.M., Davis M.A., Gordon D.C., Tarr P.I., Bartleson C.A., Lewis J.H., Barrett T.J.,Wells J.G., et al. 1994. A multistate outbreak of Escherichia coli O157:H7-associated bloody diarrhea and hemolytic uremic syndrome from hamburgers. The Washington experience. JAMA 272: 1349-1353.
3. Ake J.A., Jelacic S., Ciol M.A.,Watkins S.L., Murray K.F., Christie D.L., Klein E.J., Tarr P.I. (2005) Relative nephroprotection during Escherichia coli O157:H7 infections: association with intravenous volume expansion. Pediatrics 115: 673-680.
4. Shima K., Terajima J., Sato T., Nishimura K., Tamura K.,Watanabe H., Takeda Y., Yamasaki S. (2004) Development of a PCR-restriction fragment length polymorphism assay for the epidemiological analysis of Shiga toxin-producing Escherichia coli. J ClinMicrobiol 42: 5205-5213.
5. Shima K., Yoshii N., Akiba M., Nishimura K., Nakazawa M., Yamasaki S. (2006) Comparison of PCR-RFLP assay and PFGE for determining the clonality of enterohemorrhagic Escherichia coli strains. FEMS Microbiol Lett 257: 124-131.
6. Shima K.,Wu Y., Sugimoto N., Asakura M., Nishimura K., Yamasaki S. (2006) Comparison of PCR-restriction fragment length polymorphism (PCR-RFLP) assay to pulsed-field gel electrophoresis: effect of repeated subculture and prolonged storage on RFLP patterns of Shiga toxin-producing Escherichia coli O157:H7. J ClinMicrobiol 44: 3963-3968.
7. Khan A., Yamasaki S., Ramamurthy T., Pal A., Datta S., Chowdhury N.R., Das S.C., Sikdar A., Tsukamoto T., Bhattacharya S.K., Takeda Y., Nair G.B. (2002) Prevalence and genetic profiling of virulence determinants of non-O157 Shiga toxin-producing Escherichia coli isolated from cattle, beef and humans Calcutta, India. Emerg Infect Dis 8: 54-62.
8. Tarr P.I.,Weinberger E., Hatch E.I. Jr., Christie D.L. (1992) Bacterial ileocecitis caused by Escherichia coli O157:H7. J Pediatr Gastroenterol Nutr 14: 261-263.
9. Tarr P.I., Neill M.A., Clausen C.R.,Watkins S.L., Christie D.L., Hickman R.O. (1990) Escherichia coli O157:H7 and the hemolytic uremic syndrome: importance of early cultures in establishing the etiology. J Infect Dis 162: 553-556.
10. Michino H., Araki K., Minami S., Takaya S., Sakai N., Miyazaki M., Ono A., Yanagawa H. (1999) Massive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts. Am J Epidemiol 150: 787-796.
11. Akiba M., Sameshima T., Nakazawa M. (2000) Clonal turnover of enterohemorrhagic Escherichia coli O157 in experimentally infected cattle. FEMS Microbiol Lett 184: 79-83.
12. Karch H., Rüssmann H., Schmidt H., Schwarzkopf A., Heesemann J. (1995) Long-term shedding and clonal turnover of enterohemorrhagic Escherichia coli O157 in diarrheal diseases. J ClinMicrobiol 33: 1602-1605.
13. Iguchi A., Osawa R., Kawano J., Shimizu A., Terajima J.,Watanabe H. (2002) Effects of repeated subculturing and prolonged storage at room temperature of Enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles. J ClinMicrobiol 40: 3079-3081.