Production of human basic fibroblast growth factor (FGF-2) in Bifidobacterium breve using a series of novel expression/secretion vectors

Biotechnology Letters

Volumn 30, Issue 11, 2008, Pages 1983-1988

  Shkoporov, A N ^a   Efimov, B A ^a   Khokhlova, E V ^a   Kafarskaia, L I ^a   Smeianov, V V ^b  

^a PIROGOV RUSSIAN NATIONAL RESEARCH MEDICAL UNIVERSITY   (Russian Federation)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Bifidobacterium; Colitis; Expression; Fibroblast growth factor; Vectors

Indexed keywords

AMINES; CELL CULTURE; ESCHERICHIA COLI; GENE ENCODING; TRANSCRIPTION;

BASIC FIBROBLAST GROWTH FACTOR; BIFIDOBACTERIA; BIFIDOBACTERIUM; BIFIDOBACTERIUM LONGUM; COLITIS; E. COLI; EXPRESSION; EXPRESSION LEVELS; FIBROBLAST GROWTH FACTOR; FUSION PROTEINS; SIGNAL PEPTIDE; WESTERN BLOTTING;

VECTORS;

FIBROBLAST GROWTH FACTOR 2; RECOMBINANT PROTEIN;

ARTICLE; BIFIDOBACTERIUM; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; HUMAN; METABOLISM; PLASMID; WESTERN BLOTTING;

BIFIDOBACTERIUM; BLOTTING, WESTERN; FIBROBLAST GROWTH FACTOR 2; GENETIC VECTORS; HUMANS; PLASMIDS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

BIFIDOBACTERIUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM LONGUM; ESCHERICHIA COLI;

EID: 52449114301     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9772-8     Document Type: Article

References (16)

1. A Argnani RJ Leer N van Luijk PH Pouwels 1996 A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium Microbiology 142 109 114
2. Biavati B, Mattarelli P (2006) The family Bifidobacteriaceae. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes, vol 3. Springer, New York, pp 322-382
3. MJ Gibbs VV Smeianov JL Steele JA Upcroft BA Efimov 2006 Two families of Rep-like genes that probably originated by inter-species recombination, are represented in viral, plasmid, bacterial and parasitic protozoan genomes Mol Biol Evol 23 1097 1100
4. A Klijn D Moine M Delley A Mercenier F Arigoni RD Pridmore 2006 Construction of a reporter vector for the analysis of Bifidobacterium longum promoters Appl Environ Microbiol 72 7401 7405
5. T Kojima T Watanabe K Hata H Nagawa 2007 Basic fibroblast growth factor enema improves experimental colitis in rats Hepatogastroenterology 54 1373 1377
6. Korschunov VM, Efimov BA, Smeianov VV, Bainov NA, Pikina AP (1998) Treatment of experimental acute radiation disease in mice with probiotics, quinolones and general gnotobiological isolation. Armed Forces Radiobiology Research Institute, Defense Nuclear Agency, Bethesda, USA. Available at http://stinet.dtic.mil/oai/oai?verb=getRecord&metadataPrefix= html&identifier=ADA359101
7. LE MacConaill GF Fitzgerald D Van Sinderen 2003 Investigation of protein export in Bifidobacterium breve UCC2003 Appl Environ Microbiol 69 6994 7001
8. GS Moon YR Pyun MS Park GE Ji WJ Kim 2005 Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial alpha-amylase Appl Environ Microbiol 71 5630 5632
9. F Paris Z Fuks A Kang P Capodieci G Juan D Ehleiter A Haimovitz-Friedman C Cordon-Cardo R Kolesnick 2001 Endothelial apoptosis as the primary lesion initiating intestinal radiation damage in mice Science 293 293 297
10. ML Reyes Escogido A De León Rodríguez AP Barba de la Rosa 2007 A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum Biotechnol Lett 29 1249 1253
11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
12. MA Schell M Karmirantzou B Snel D Vilanova B Berger G Pessi MC Zwahlen F Desiere P Bork M Delley RD Pridmore F Arigoni 2002 The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract Proc Natl Acad Sci USA 99 14422 14427
13. A Seeger B Schneppe JEG McCarthy WD Deckwer U Rinas 1995 Comparison of temperature- and isopropyl-beta-D-thiogalactopyranoside-induced synthesis of basic fibroblast growth factor in high-cell-density cultures of recombinant Escherichia coli Enzyme Microb Technol 17 947 953
14. Smeianov VV, Efimov BA, Korschunov VM, Steele JL (2002) Construction of the E. coli-Bifidobacterium shuttle vectors based on two distinctive Bifidobacterium replicons. American Society for Microbiology 102nd General Meeting, Salt Lake City, Utah:H-7
15. T Takata T Shirakawa Y Kawasaki S Kinoshita A Gotoh Y Kano M Kawabata 2006 Genetically engineered Bifidobacterium animalis expressing the Salmonella flagellin gene for the mucosal immunization in a mouse model J Gene Med 8 1341 1346
16. A Takeuchi H Matsumura Y Kano 2002 Cloning and expression in Escherichia coli of a gene, hup, encoding the histone-like protein HU of Bifidobacterium longum Biosci Biotechnol Biochem 66 598 603