On the stereochemical course of human protein-farnesyl transferase

Journal of the American Chemical Society

Volumn 118, Issue 8, 1996, Pages

  Mu, YongQi ^a   Omer, Charles A ^b   Gibbs, Richard A ^a  

^a WAYNE STATE UNIVERSITY   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 5244300102     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

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23. The Boc-protected amino acid reagents were from Bachem, and all other synthetic reagents were from Aldrich unless otherwise noted. Solvents were from Aldrich, Fisher, or Curtin-Matheson and were used as received unless otherwise indicated. HPLC work was carried out using HPLC-grade solvents on a Waters system consisting of a U6K injector, two 501 pumps, and a 490E multiwavelength UV detector.
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43. Note that if partial but significant racemization occurred during the PFTase reaction, then the diasteromeric excess of the biosynthetic 4 must he less than the 62% calculated for the synthetic sample of 3, since both are derived from the same sample of farnesyl chloride 14. In other words, this would mean that the minor negative peak in Figure 2c (at 2.807 ppm) would be larger than the minor negative peak in Figure 2b (at 2.825 ppm). Despite the significant noise level in Figure 2c, this does not appear to be the case, as such a peak would be more clearly visible above the noise. Thus, although we are only able to provide estimates of the diasteromeric excess of the biosynthetic farnesylated peptides, visual inspection of the spectra bolsters our confidence that the enzymatic transfer is accompanied by little if any racemization.
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