Propidium iodide-based methods for monitoring drug action in the kinetoplastidae: Comparison with the Alamar Blue assay

Analytical Biochemistry

Volumn 382, Issue 2, 2008, Pages 87-93

  Gould, Matthew K ^a   Vu, Xuan Lan ^b   Seebeck, Thomas ^b   de Koning, Harry P ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Alamar Blue assay; Drug action; Kinetoplastidae; Propidium iodide

Indexed keywords

CELL DEATH;

CELL VIABILITY; DRUG ACTION; DRUG DEVELOPMENT; FLUORIMETRIC ASSAYS; HIGH-THROUGHPUT ASSAYS; KINETOPLASTIDAE; PROPIDIUM IODIDE; REAL TIME MONITORING;

DRUG PRODUCTS;

PROPIDIUM IODIDE; RESAZURIN;

AFRICAN TRYPANOSOMIASIS; ARTICLE; CELL VIABILITY; COMPARATIVE STUDY; CORRELATION ANALYSIS; DNA CONTENT; DRUG MONITORING; FLAGELLATE; GENE INTERACTION; HIGH THROUGHPUT SCREENING; PRIORITY JOURNAL;

EID: 52349084715     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.07.036     Document Type: Article

References (27)

1. Jannin J., and Cattand P. Treatment and control of human African trypanosomiasis. Curr. Opin. Infect. Dis. 17 (2004) 565-570
2. Delespaux V., and De Koning H.P. Drugs and drug resistance in African trypanosomiasis. Drug Resist. Updat. 10 (2007) 30-50
3. Matovu E., Enyaru J.C.K., Legros D., Schmid C., Seebeck T., and Kaminsky R. Melarsoprol refractory T. b. gambiense from Omugo, north-western Uganda. Trop. Med. Int. Health 6 (2001) 407-411
4. Brun R., Schumacher R., Schmid C., Kunz C., and Burri C. The phenomenon of treatment failures in human African trypanosomiasis. Trop. Med. Int. Health 6 (2001) 906-914
5. Geerts S., Holmes P.H., Diall O., and Eisler M.C. African bovine trypanosomiasis: the problem of drug resistance. Trends Parasitol. 17 (2001) 25-28
6. Räz B., Iten M., Grether-Bühler Y., Kaminsky R., and Brun R. The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T. b. rhodesiense and T. b. gambiense) in vitro. Acta Tropica 68 (1997) 139-147
7. Gonzalez R.J., and Tarloff J.B. Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicol. In Vitro 15 (2001) 257-259
8. Slaughter M.R., Bugelski P.J., and O'Brien J. Evaluation of Alamar blue reduction for the in vitro assay of hepatocyte toxicity. Toxicol. In vitro 13 (1999) 567-569
9. White M.J., DiCaprio M.J., and Greenberg D.A. Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures. J. Neurosci. Methods 70 (1996) 95-200
10. Pfaller M.A., Vu Q., Lancaster M., Espinel-Ingroff A., Fothergill A., Grant C., McGinnis M.R., Pasarell L., Rinaldi M.G., and Steele-Moore L. Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. J. Clin. Microbiol. 32 (1994) 1625-1628
11. Mikus J., and Steverding D. A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue. Parasitol. Int. 48 (2000) 265-269
12. Al-Salabi M.I., and De Koning H.P. Purine nucleobase transport in amastigotes of Leishmania mexicana: involvement in allopurinol uptake. Antimicrob. Agents Chemother. 49 (2005) 3682-3689
13. Rolón M., Vega C., Escario J.A., and Gomez-Barrio A. Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes. Parasitol. Res. 99 (2006) 103-107
14. Eichler H. Der Nachweis der hydrosulfite (sulfoxylate) und des nachierenden Wasserstoffs mit Resazurin, Fresenius'. J. Anal. Chem. 99 (1934) 270-272
15. O'Brien J., Wilson I., Orton T., and Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell toxicity. Eur. J. Biochem. 267 (2000) 5421-5426
16. 6-Disubstituted adenosine analogues with antitrypanosomal and antimalarial activity; synthesis, uptake studies and in vivo evaluation. Antimicrob. Agents Chemother. 51 (2007) 3796-3802
17. A.L. Foucher, A proteomic approach to the investigation of cymelarsan resistance in Trypanosoma brucei. PhD thesis, University of Glasgow, UK, 2003.
18. Ferlini C., Di Cesare S., Rainalldi G., Malorni W., Samoggia P., Biselli R., and Fattorossi A. Flow cytometric analysis of the early phases of apoptosis by cellular and nuclear techniques. Cytometry 24 (1996) 106-115
19. Medina-Acosta E., and Cross G. Rapid isolation of DNA from trypanosomatid protozoa using a simple mini-prep procedure. Mol. Biochem. Parasitol. 59 (1993) 327-330
20. Bridges D., Gould M.K., Nerima B., Mäser P., Burchmore R.J.S., and De Koning H.P. Loss of the High Affinity Pentamidine Transporter is responsible for high levels of cross-resistance between arsenical and diamidine drugs in African trypanosomes. Mol. Pharmacol 71 (2007) 1098-1108
21. Melville S.E., Leech V., Navarro M., and Cross G.A.M. The molecular karyotype of the megabase chromosomes of the Trypanosoma brucei stock 427. Mol. Biochem. Parasitol. 111 (2000) 261-273
22. M. Berriman, E. Ghedin, C. Hertz-Fowler, G. Blandin, H. Renauld, D.C. Bartholomeu, N.J. Lennard, E. Caler, N.E. Hamlin, B. Haas, U. Böhme, L. Hannick, M.A. Aslett, J. Shallom, L. Marcello, L. Hou, B. Wickstead, U.C. Alsmark, C. Arrowsmith, R.J. Atkin, A.J. Barron, F. Bringaud, K. Brooks, M. Carrington, I. Cherevach, T.J. Chillingworth, C. Churcher, L.N. Clark, C.H. Corton, A. Cronin, R.M. Davies, J. Doggett, A. Djikeng, T. Feldblyum, M.C. Field, A. Fraser, I. Goodhead, Z. Hance, D. Harper, B.R. Harris, H. Hauser, J. Hostetler, A. Ivens, K. Jagels, D. Johnson, J. Johnson, K. Jones, A.X. Kerhornou, H. Koo, N. Larke, S. Landfear, C. Larkin, V. Leech, A. Line, A. Lord, A. Macleod, P.J. Mooney, S. Moule, D.M. Martin, G.W. Morgan, K. Mungall, H. Norbertczak, D. Ormond, G. Pai, C.S. Peacock, J. Peterson, M.A. Quail, E. Rabbinowitsch, M.A. Rajandream, C. Reitter, S.L. Salzberg, M. Sanders, S. Schobel, S. Sharp, M. Simmonds, A.J. Simpson, L. Tallon, C.M. Turner, A. Tait, A.R. Tivey, S. Van Aken, D. Walker, D. Wanless, S. Wang, B. White, O. White, S. Whitehead, J. Woodward, J. Wortman, M.D. Adams, T.M. Embley, K. Gull, E. Ullu, J.D. Barry, A.H. Fairlamb, F. Opperdoes, B.G. Barrell, J.E. Donelson, N. Hall, C.M. Fraser, S.E. Melville, N.M. El-Sayed, The genome of the African trypanosome Trypanosoma brucei, Science 309 (2005) 416-422.
23. De Koning H.P., Watson C.J., and Jarvis S.M. Characterisation of a nucleoside/proton symporter in procyclic Trypanosoma brucei brucei. J. Biol. Chem. 273 (1998) 9486-9494
24. Matovu E., Stewart M., Geiser F., Brun R., Mäser P., Wallace L.J.M., Burchmore R.J., Enyaru J.C.K., Barrett M.P., Kaminsky R., Seebeck T., and De Koning H.P. The mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei. Eukaryot. Cell 2 (2003) 1003-1008
25. Fairlamb A.H., Carter N.S., Cunningham M., and Smith K. Characterisation of melarsen-resistant Trypanosoma brucei brucei with respect to cross-resistance to other drugs and trypanothione metabolism. Mol. Biochem. Parasitol. 53 (1992) 213-222
26. Papageorgiou I.G., Yakob L., Al Salabi M.I., Diallinas G., Soteriadou K., and De Koning H.P. Identification of the first pyrimidine nucleobase transporter in Leishmania: similarities with the Trypanosoma brucei U1 transporter and antileishmanial activity of uracil analogues. Parasitology 130 (2005) 275-283
27. Wallace L.J.M., Candlish D., and De Koning H.P. Different substrate recognition motifs of human and trypanosome nucleobase transporters: selective uptake of purine antimetabolites. J. Biol. Chem. 277 (2002) 26149-26156