Modification of amyloid-β(1-40) by a protease inhibitor creates risk of error in mass spectrometric quantitation of amyloid-β(1-42)

Analytical Biochemistry

Volumn 382, Issue 2, 2008, Pages 147-149

  Conboy, James J ^a   Wood, Kathleen G ^a   Lame, Mary E ^a   Durham, Robert A ^a   Geoghegan, Kieran F ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DRUG PRODUCTS; FLUORINE COMPOUNDS; GLYCOPROTEINS; MAMMALS; MASS SPECTROMETRY;

AMYLOID BETAS; DRUG ACTION; GUINEA PIGS; MATRIX ASSISTED LASER DESORPTION; NOVEL COMPONENT; PROTEASE INHIBITOR;

PROTEINS;

AMYLOID BETA PROTEIN[1-40]; AMYLOID BETA PROTEIN[1-42]; PROTEINASE INHIBITOR;

ANIMAL TISSUE; ARTICLE; ERROR; GUINEA PIG; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; QUANTITATIVE ASSAY;

CAVIA;

EID: 52049124015     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.07.033     Document Type: Article

References (5)

1. Gandy S. The role of cerebral amyloid β accumulation in common forms of Alzheimer disease. J. Clin. Invest. 115 (2005) 1121-1129
2. Wang R., Sweeney D., Gandy S.E., and Sisodia S.S. The profile of soluble amyloid β protein in cultured cell media. Detection and quantification of amyloid β protein and variants by immunoprecipitation-mass spectrometry. J. Biol. Chem. 271 (1996) 31894-31902
3. Du P., Wood K.M., Rosner M.H., Cunningham D., Tate B., and Geoghegan K.F. Dominance of amyloid precursor protein sequence over host cell secretases in determining β-amyloid profiles studies of interspecies variation and drug action by internally standardized immunoprecipitation/mass spectrometry. J. Pharmacol. Exp. Ther. 320 (2007) 1144-1152
4. Bourell J.H., Stults J.T., Gonzalez T., Pearce K., and Vandlen R.L. Frequent undesired covalent modification of proteins by the protease inhibitor 4-(2-aminoethyl)-benzenesulfonylfluoride (AEBSF, Pefabloc). Protein Sci. 4 Suppl. 2 (1995) 148
5. Ford M.J., Cantone J.L., Polson C., Toyn J.H., Meredith J.E., and Drexler D.M. Qualitative and quantitative characterization of the amyloid β peptide (Aβ) population in biological matrices using an immunoprecipitation-LC/MS assay. J. Neurosci. Methods 168 (2008) 465-474