Chapter 13 Assessment of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation Products

Methods in Enzymology

Volumn 441, Issue , 2008, Pages 237-260

  Laurindo, Francisco R M ^a   Fernandes, Denise C ^a   Santos, Célio X C ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; HYDROETHIDINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; SUPEROXIDE; DRUG DERIVATIVE; ETHIDIUM;

ACCURACY; CARDIOVASCULAR SYSTEM; CELL CULTURE; CELL MEMBRANE; ENZYME ACTIVITY; FLUORESCENCE; FLUOROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OXIDATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVIEW; ULTRAVIOLET RADIATION; ANIMAL; CHEMICAL ANALYSIS; HUMAN; METABOLISM; METHODOLOGY; OXIDATION REDUCTION REACTION; PHYSIOLOGY;

ANIMALS; CELLS, CULTURED; CHEMISTRY TECHNIQUES, ANALYTICAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ETHIDIUM; HUMANS; NADPH OXIDASE; OXIDATION-REDUCTION; SUPEROXIDES;

EID: 52049110957     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)01213-5     Document Type: Chapter

References (40)

1. Beckman J.S., Minor Jr. R.L., White C.W., Repine J.E., Rosen G.M., and Freeman B.A. Superoxide dismutase and catalase conjugated to polyethylene glycol increases endothelial enzyme activity and oxidant resistance. J. Biol. Chem. 263 (1988) 6884-6892
2. Beckmann J.S., Ye Y.Z., Anderson P.G., Chen J., Accavitti M.A., Tarpey M.M., and White C.R. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol. Chem. Hoppe Seyler 375 (1994) 81-88
3. Benov L., Sztejnberg L., and Fridovich I. Critical evaluation of the use of hydroethidine as a measure of superoxide anion radical. Free Radic. Biol. Med. 25 (1998) 826-831
4. Biemond P., Swaak A.J., Beindorff C.M., and Koster J.F. Superoxide-dependent and -independent mechanisms of iron mobilization from ferritin by xanthine oxidase: Implications for oxygen-free-radical-induced tissue destruction during ischaemia and inflammation. Biochem. J. 239 (1986) 169-173
5. Bindokas V.P., Jordan J., Lee C.C., and Miller R.J. Superoxide production in rat hippocampal neurons: Selective imaging with hydroethidine. J. Neurosci. 16 (1996) 1324-1336
6. Cadenas E., Mira D., Brunmark A., Lind C., Segura-Aguilar J., and Ernster L. Effect of superoxide dismutase on the autoxidation of various hydroquinones: A possible role of superoxide dismutase as a superoxide: Semiquinone oxidoreductase. Free. Radic. Biol. Med. 5 (1988) 71-79
7. Cave A.C., Brewer A.C., Narayanapanicker A., Ray R., Grieve D.J., Walker S., and Shah A.M. NADPH oxidases in cardiovascular health and disease. Antioxid. Redox. Signal. 8 (2006) 691-728
8. Chen K., Thomas S.R., and Keaney Jr. J.F. Beyond LDL oxidation: ROS in vascular signal transduction. Free Radic. Biol. Med. 35 (2003) 117-132
9. Clempus R.E., and Griendling K.K. Reactive oxygen species signaling in vascular smooth muscle cells. Cardiovasc. Res. 71 (2006) 216-225
10. Dikalov S., Griendling K.K., and Harrison D.G. Measurement of reactive oxygen species in cardiovascular studies. Hypertension 49 (2007) 717-727
11. Fernandes D.C., Wosniak J.J., Pescatore L.A., Bertoline M.A., Liberman M., Laurindo F.R., and Santos C.X.C. Analysis of dihydroethidium-derived oxidation products by HPLC in the assessment of superoxide production and NADPH oxidase activity in vascular systems. Am. J. Physiol. Cell. Physiol. 292 (2007) C413-C422
12. Fink B., Laude K., McCann L., Doughan A., Harrison D.G., and Dikalov S. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Am. J. Physiol. Cell. Physiol. 287 (2004) C895-C902
13. Forman H.J., Fukuto J.M., and Torres M. Redox signaling: Thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers. Am. J. Physiol. Cell. Physiol. 287 (2004) C246-C256
14. Garbett N.C., Hammond N.B., and Graves D.E. Influence of the amino substituents in the interaction of ethidium bromide with DNA. Biophys. J. 87 (2004) 3974-3981
15. Gardner P.R., and Fridovich I. Inactivation-reactivation of aconitase in Escherichia coli: A sensitive measure of superoxide radical. J. Biol. Chem. 267 (1992) 8757-8763
16. Kissner R., Nauser T., Bugnon P., Lye P.G., and Koppenol W.H. Formation and properties of peroxynitrite as studied by laser flash photolysis, high-pressure stopped-flow technique, and pulse radiolysis. Chem. Res. Toxicol. 10 (1997) 1285-1292
17. Halliwell B., and Gutteridge J.M.C. The chemistry of free radicals and related "reactive species". Free Radicals in Biology and Medicine. 3rd Ed. (1998), Oxford University Press, New York 62
18. Hwang J., Saha A., Boo Y.C., Sorescu G.P., McNally J.S., Holland S.M., Dikalov S., Giddens D.P., Griendling K.K., Harrison D.G., and Jo H. Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion. J. Biol. Chem. 278 (2003) 47291-47298
19. Janiszewski M., de Sousa H.P., Liu X., Pedro M.A., Zweier J.L., and Laurindo F.R. Overestimation of NADH-driven vascular oxidase activity due to lucigenin artifacts. Free Radic. Biol. Med. 32 (2002) 446-453
20. Lassegue B., and Clempus R.E. Vascular NAD(P)H oxidases: Specific features, expression, and regulation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 285 (2003) R277-R297
21. Laurindo F.R.M., Souza H.P., Pedro M.P., and Janiszewski M. Redox aspects of vascular response to injury. Methods Enzymol. 352 (2002) 432-454
22. Li H., Witte K., August M., Brausch I., Godtel-Armbrust U., Habermeier A., Closs E.I., Oelze M., Munzel T., and Forstermann U. Reversal of endothelial nitric oxide synthase uncoupling and up-regulation of endothelial nitric oxide synthase expression lowers blood pressure in hypertensive rats. J. Am. Coll. Cardiol. 47 (2006) 2536-2544
23. Li J., Stouffs M., Serrander L., Banfi B., Bettiol E., Charnay Y., Steger K., Krause K.H., and Jaconi M.E. The NADPH oxidase NOX4 drives cardiac differentiation: Role in regulating cardiactranscription factors and MAP kinase activation. Mol. Biol. Cell. 17 (2006) 3978-3988
24. Miller Jr. F.J., Filali M., Huss G.J., Stanic B., Chamseddine A., Barna T.J., and Lamb F.S. Cytokine activation of nuclear factor kappa B in vascular smooth muscle cells requires signaling endosomes containing Nox1 and ClC-3. Circ. Res. 101 (2007) 663-671
25. Miller Jr. F.J., Gutterman D.D., Rios C.D., Heistad D.D., and Davidson B.L. Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis. Circ. Res. 82 (1998) 1298-1305
26. Nemoto S., Takeda K., Yu Z.X., Ferrans V.J., and Finkel T. Role for mitochondrial oxidants as regulators of cellular metabolism. Mol. Cell. Biol. 20 (2000) 7311-7318
27. Oak J.H., and Cai H. Attenuation of angiotensin II signaling recouples eNOS and inhibits nonendothelial NOX activity in diabetic mice. Diabetes 56 (2007) 118-126
28. Papapostolou I., Patsoukis N., and Georgiou C.D. The fluorescence detection of superoxide radical using hydroethidine could be complicated by the presence of heme proteins. Anal. Biochem. 332 (2004) 290-298
29. 2. Anal. Bioanal. Chem. 381 (2005) 1065-1072
30. Robinson K.M., Janes M.S., Pehar M., Monette J.S., Ross M.F., Hagen T.M., Murphy M.P., and Beckman J.S. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc. Natl. Acad. Sci. USA 103 (2006) 15038-15043
31. Souza H.P., Liu X., Samouilov A., Kuppusamy P., Laurindo F.R.M., and Zweier J.L. Quantitation of superoxide generation and substrate utilization by vascular NAD(P)H oxidase. Am. J. Physiol. (Heart Circ. Physiol). 282 (2002) H466-H474
32. Spasojevic I., Liochev S.I., and Fridovich I. Lucigenin: Redox potential in aqueous media and redox cycling with O-(2) production. Arch. Biochem. Biophys. 373 (2000) 447-450
33. Tarpey M.M., Wink D.A., and Grisham M.B. Methods for detection of reactive metabolites of oxygen and nitrogen: In vitro and in vivo considerations. Am. J. Physiol. Regul. Integr. Comp. Physiol. 286 (2004) 431-444
34. Thompson R.J., Akana H.C., Finnigan C., Howell K.E., and Caldwell J.H. Anion channels transport ATP into the Golgi lumen. Am. J. Physiol. Cell Physiol. 290 (2006) C499-C514
35. Vasquez-Vivar J., Kalyanaraman B., Martasek P., Hogg N., Masters B.S., Karoui H., Tordo P., and Pritchard Jr. K.A. Superoxide generation by endothelial nitric oxide synthase: The influence of cofactors. Proc. Natl. Acad. Sci. USA 95 (1998) 9220-9225
36. Zhao H., Joseph J., Fales H.M., Sokoloski E.A., Levine R.L., Vasquez-Vivar J., and Kalyanaraman B. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc. Natl. Acad. Sci. USA 102 (2005) 5727-5732
37. Zhao H., Kalivendi S., Zhang H., Joseph J., Nithipatikom K., Vasquez-Vivar J., and Kalyanaraman B. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: Potential implications in intracellular fluorescence detection of superoxide. Free Radic. Biol. Med. 34 (2003) 1359-1368
38. Zielonka J., Sarna T., Roberts J.E., Wishart J.F., and Kalyanaraman B. Pulse radiolysis and steady-state analyses of the reaction between hydroethidine and superoxide and other oxidants. Arch. Biochem. Biophys. 456 (2006) 39-47
39. Zielonka J., Vasquez-Vivar J., and Kalyanaraman B. The confounding effects of light, sonication, and Mn(III)TBAP on quantitation of superoxide using hydroethidine. Free Radic. Biol. Med. 41 (2006) 1050-1057
40. Zielonka J., Zhao H., Xu Y., and Kalyanaraman B. Mechanistic similarities between oxidation of hydroethidine by Fremy's salt and superoxide: Stopped-flow optical and EPR studies. Free Radic. Biol. Med. 39 (2005) 853-863