Cloning expression and analysis of phytochelatin synthase (pcs) gene from Anabaena sp. PCC 7120 offering multiple stress tolerance in Escherichia coli

Biochemical and Biophysical Research Communications

Volumn 376, Issue 1, 2008, Pages 225-230

  Chaurasia, Neha ^a   Mishra, Yogesh ^a   Rai, Lal Chand ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Abiotic stress; Anabaena sp. PCC 7120; Cloning; Phytochelatin synthase (PCS); RT PCR

Indexed keywords

CADMIUM; COPPER; PESTICIDE; PHYTOCHELATIN; PHYTOCHELATIN SYNTHASE; SODIUM CHLORIDE;

ABIOTIC STRESS; ANABAENA; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRAVIOLET B RADIATION;

AMINOACYLTRANSFERASES; ANABAENA; CADMIUM CHLORIDE; CLONING, MOLECULAR; COPPER; ESCHERICHIA COLI; GENES, PLANT; HEAT; PHYTOCHELATINS; SODIUM CHLORIDE; ULTRAVIOLET RAYS;

ESCHERICHIA COLI; NOSTOC SP. PCC 7120;

EID: 52049107054     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.08.129     Document Type: Article

References (15)

1. Bowler C., Van Montagu M., and Inze D. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43 (1992) 83-116
2. Grill E., Loffler S., Winnacker E.L., and Zenk M.H. Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase). Proc. Natl. Acad. Sci. USA 86 (1989) 6838-6842
3. Tsuji N., Hirayanagi N., Okada M., Miyasaka H., Hirata K., Zenk M., and Miyamoto K. Enhancement of tolerance to heavy metals and oxidative stress in Dunaliella tertiolecta by Zn-induced phytochelatin synthesis. Biochem. Biophys. Res. Commun. 293 (2002) 653-659
4. 2-fixing cyanobacterium Anabaena doliolum. J. Plant Physiol. 162 (2005) 1220-1225
5. Ha S.-B., Smith A.P., Howden R., Dietrich W.M., Bugg S., O'Connell M.J., Goldsbrough P.B., and Cobbett C.S. Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell 11 (1999) 1153-1163
6. Vatamaniuk O.K., Mari S., Lu Y.-P., and Rea P.A. AtPCS1, a phytochelatin synthase from Arabidopsis thaliana: isolation and in vitro reconstitution. Proc. Natl. Acad. Sci. USA 96 (1999) 7110-7115
7. Clemens S., Kim E.U., Neumann D., and Schroeder J.L. Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J. 18 (1999) 3325-3333
8. 2 fixing cyanobacterium Anabaena doliolum with special reference to co-/multiple stress tolerance. Biometals 11 (1998) 55-61
9. Danilin I.A., Dikarev V.G., and Geraskin S.A. The radiation-increased synthesis of phytochelatins in roots of gamma-irradiated barley seedlings. Radiats. Biol. Radioecol. 44 (2004) 89-92
10. Zhang H., Xu W., Guo J., He Z., and Ma M. Coordinated responses of phytochelatins and metalothioneins to heavy metals in garlic seedlings. Plant Sci. 169 (2005) 1059-1065
11. Rippka R., Deruelles J., Waterbury J.B., Herdman M., and Stanier R.Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111 (1979) 1-6
12. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
13. Srivastava A.K., Ara A., Bhargava P., Mishra Y., Rai S.P., and Rai L.C. A rapid and cost-effective method of genomic DNA isolation from cyanobacterial culture, mat and soil suitable for genomic fingerprinting and community analysis. J. Appl. Phycol. 19 (2007) 373-382
14. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantity of proteins utilising the principle of protein dye binding. Anal. Biochem. 72 (1976) 248-254
15. Rabenstein D.L. Metal complexes of glutathione and their biological significance. In: Dolphin D., Poulson R., and Avramovic O. (Eds). Glutathione: Chemical, Biochemical and Medical Aspects (1989), John Wiley and Sons, New York 147-186