Conditioned media cause increases in select osteogenic and adipogenic differentiation markers in mesenchymal stem cell cultures

Journal of Tissue Engineering and Regenerative Medicine

Volumn 2, Issue 2-3, 2008, Pages 147-154

  Maxson, Scott ^a   Burg, Karen J L ^a  

^a CLEMSON UNIVERSITY   (United States)

Author keywords

Adipogenic differentiation; Conditioned media; Mesenchymal stem cells; Osteogenic differentiation; Tissue engineering

Indexed keywords

CELL ENGINEERING; FLOWCHARTING; METABOLISM; MOLECULAR WEIGHT; OSTEOBLASTS; PHOSPHATASES; STEM CELLS; TISSUE; TISSUE ENGINEERING;

ADIPOCYTES; ADIPOGENIC DIFFERENTIATIONS; CONDITIONED MEDIUM; ENGINEERED DEVICES; MESENCHYMAL STEM CELL; METABOLIC ACTIVITY; OSTEOGENIC DIFFERENTIATION; STEM CELL CULTURE; STEM CELL DIFFERENTIATION; TISSUES ENGINEERINGS;

CELL CULTURE;

ALKALINE PHOSPHATASE; BIOLOGICAL MARKER; TRIACYLGLYCEROL; AZO COMPOUND; OIL RED O;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL METABOLISM; CONTROLLED STUDY; DEVICE; ENZYME ASSAY; LIPID VESICLE; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; OSTEOBLAST; PARACRINE SIGNALING; PRIORITY JOURNAL; TISSUE ENGINEERING; ADIPOGENESIS; ANIMAL; BONE DEVELOPMENT; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; METABOLISM;

ADIPOGENESIS; ALKALINE PHOSPHATASE; ANIMALS; AZO COMPOUNDS; BIOLOGICAL MARKERS; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; MESENCHYMAL STEM CELLS; MICE; OSTEOGENESIS; TRIGLYCERIDES;

EID: 52049087188     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.76     Document Type: Article

References (33)

1. Ailhaud G, Grimaldi P, Negrel R. 1992; Cellular and molecular aspects of adipose-tissue development. Annu Rev Nutr 12: 207-233.
2. Akune T, Ohba S, Kamekura S, et al. 2004; PPAR insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. J Clin Invest 113: 846-855.
3. Alhadlaq A, Elisseeff JH, Hong L, et al. 2004; Adult stem cell driven genesis of human-shaped articular condyle. Ann Biomed Eng 32: 911-923.
4. Benayahu D, Zipori D, Wientroub S. 1993; Marrow adipocytes regulate growth and differentiation of osteoblasts. Biochem Biophys Res Commun 197: 1245-1252.
5. Beresford JN, Bennett JH, Devil C, et al. 1992; Evidence for an inverse relationship between the differentiation of adipocytes and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci 102: 341-351.
6. Blair HC, Athanasou NA. 2004; Recent advances in osteoclast biology and pathological bone resorption. Histol Histopathol 19(1): 189-199.
7. Bornstein SR, Licinio J, Tauchnitz R, et al. 1998; Plasma leptin levels are increased in survivors of acute sepsis: associated loss of diurnal rhythm in cortisol and leptin secretion. J Clin Endocrinol Metab 83: 280-283.
8. Centrella M, McCarthy TL, Canalis E. 1988; Tumor necrosis factoralpha inhibits collagen synthesis and alkaline-phosphatase activity independently of its effect on deoxyribonucleic acid synthesis in osteoblast-enriched bone cell cultures. Endocrinology 123: 1442-1448.
9. Chen D, Ji X, Harris MA, et al. 1998; Differential roles of bone morphogenetic protein (BMP) receptor type IB and IA in differentiation and specification of mesenchymal precursor cells to osteoblast and adipocyte lineages. J Cell Biol 142: 295-305.
10. Coppack SW, Pinkney JH, Mohamed-Ali V. 1998; Leptin production in human adipose tissue. Proc Nutr Soc 57: 461-470.
11. Dorheim MA, Dandapani V, Wu XY, et al. 1993; Osteoblastic gene-expression during adipogenesis in hematopoietic supporting murine bone-marrow stromal cells. J Cell Physiol 154: 317-328.
12. Fried SK, Bunkin DA, Greenberg AS. 1998; Omental and subcutaneous adipose tissues of obese subjects release interleukin-6: depot difference and regulation by glucocorticoid. J Clin Endocrinol Metab 83: 847-850.
13. Gimble J. 1990; The function of adipocytes in the bone marrow stroma. New Biol 2: 304-312.
14. Gimble JM, Guilak F. 2003; Differentiation potential of adipose derived adult stem (ADAS) cells. Curr Top Dev Biol 58: 137-160.
15. Gimble JM, Nuttall ME. 2004; Bone and fat: old questions, new insights. Endocrine 23: 183-188.
16. Gimble JM, Robinson CE, Wu X, et al. 1996; The function of adipocytes in the bone marrow stroma: an update. Bone 19: 421-428.
17. Gimble JM, Zvonic S, Floyd ZE, et al. 2006; Playing with bone and fat. J Cell Biochem 98: 251-266.
18. Gomillion CT, Burg KJL. 2006; Stem cells and adipose tissue engineering. Biomaterials 27: 6052-6063.
19. Gowen M, Macdonald BR, Russell RGG. 1988; Actions of recombinant human γ-interferon and tumor necrosis factor-α on the proliferation and osteoblastic characteristics of human trabecular bone cells in vitro. Arthritis Rheum 31: 1500-1507.
20. Hotamisligil GS, Shargill NS, Spiegelman BM. 1993; Adipose expression of tumor necrosis factor-α - direct role in obesity-linked insulin resistance. Science 259: 87-91.
21. Ji XHCD, Xu C, Harris SE, et al. 2000; Patterns of gene expression associated with BMP-2-induced osteoblast and adipocyte differentiation of mesenchymal progenitor cell 3T3-F442A. J Bone Miner Metab 18: 132-139.
22. Krampera M, Pizzolo G, Aprili G, et al. 2006; Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone 39: 678-683.
23. Laharrague P, Larrouy D, Fontanilles AM, et al. 1998; High expression of leptin by human bone marrow adipocytes in primary culture. FASEB J 12: 747-752.
24. Maurin AC, Chavassieux PM, Frappart L, et al. 2000; Influence of mature adipocytes on osteoblast proliferation in human primary cocultures. Bone 26: 485-489.
25. Mundy GR, Boyce BF, Yoneda T, et al. 1996; Cytokine and bone remodeling. Osteoporosis 6: 301-313.
26. Oshina H, Sotome S, Yoshii T, et al. 2007; Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Bone 41(4): 575-583.
27. Polak JM, Bishop AE. 2006; Stem cells and tissue engineering: past, present, and future. Ann N Y Acad Sci 1068: 352-366.
28. Porter RM, Huckle WR, Goldstein AS. 2003; Effect of dexamethasone withdrawal on osteoblastic differentiation of bonemarrow stromal cells. J Cell Biochem 90: 13-22.
29. Presnell SC, Petersen B, Heidaran M. 2002; Stem cells in adult tissues. Semin Cell Dev Biol 13: 369-376.
30. Raghunath J, Salacinski HJ, Sales KM, et al. 2005; Advancing cartilage tissue engineering: the application of stem cell technology. Curr Opin Biotechnol 16: 503-509.
31. Rahaman MN, Mao JJ. 2005; Stem cell-based composite tissue constructs for regenerative medicine. Biotechnol Bioeng 91: 261-284.
32. Thomas T, Gori F, Khosla S, et al. 1999; Leptin acts on human marrow stromal cells to enhance differentiation to osteoblasts and to inhibit differentiation to adipocytes. Endocrinology 140: 1630-1638.
33. Yokota TMC, Medina KL, Igarashi H, et al. 2002; Paracine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins. J Clin Invest 109: 1303-1310.