The Anchor-Away Technique: Rapid, Conditional Establishment of Yeast Mutant Phenotypes

Molecular Cell

Volumn 31, Issue 6, 2008, Pages 925-932

  Haruki, Hirohito ^a   Nishikawa, Junichi ^a   Laemmli, Ulrich K ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

CELLBIO; DNA; RNA

Indexed keywords

PROTEIN SUBUNIT; RAPAMYCIN; RIBOSOME PROTEIN; RNA;

ARTICLE; CELL NUCLEUS; CYTOPLASM; DIMERIZATION; GENE FUNCTION; GENE SILENCING; GENE STRUCTURE; GENETIC TRANSCRIPTION; NONHUMAN; PHENOTYPE; SISTER CHROMATID; YEAST;

CELL NUCLEUS; CELL PROLIFERATION; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; GENETIC TECHNIQUES; MUTAGENESIS; PHENOTYPE; RIBOSOMES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIROLIMUS; TIME FACTORS; TRANSCRIPTION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 52049084405     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2008.07.020     Document Type: Article

References (24)

1. Arevalo-Rodriguez M., Wu X., Hanes S.D., and Heitman J. Prolyl isomerases in yeast. Front. Biosci. 9 (2004) 2420-2446
2. Belshaw P.J., Ho S.N., Crabtree G.R., and Schreiber S.L. Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins. Proc. Natl. Acad. Sci. USA 93 (1996) 4604-4607
3. Chen J., Zheng X.F., Brown E.J., and Schreiber S.L. Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDa FKBP12-rapamycin-associated protein and characterization of a critical serine residue. Proc. Natl. Acad. Sci. USA 92 (1995) 4947-4951
4. Dohmen R.J., Wu P., and Varshavsky A. Heat-inducible degron: a method for constructing temperature-sensitive mutants. Science 263 (1994) 1273-1276
5. Enenkel C., Blobel G., and Rexach M. Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J. Biol. Chem. 270 (1995) 16499-16502
6. Ghaemmaghami S., Huh W.K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., and Weissman J.S. Global analysis of protein expression in yeast. Nature 425 (2003) 737-741
7. Heitman J., Movva N.R., and Hall M.N. Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast. Science 253 (1991) 905-909
8. Ishii K., Arib G., Lin C., Van Houwe G., and Laemmli U.K. Chromatin boundaries in budding yeast: the nuclear pore connection. Cell 109 (2002) 551-562
9. Janse D.M., Crosas B., Finley D., and Church G.M. Localization to the proteasome is sufficient for degradation. J. Biol. Chem. 279 (2004) 21415-21420
10. Klemm J.D., Beals C.R., and Crabtree G.R. Rapid targeting of nuclear proteins to the cytoplasm. Curr. Biol. 7 (1997) 638-644
11. Kohler A., and Hurt E. Exporting RNA from the nucleus to the cytoplasm. Nat. Rev. Mol. Cell Biol. 8 (2007) 761-773
12. Laurent B.C., Treitel M.A., and Carlson M. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation. Proc. Natl. Acad. Sci. USA 88 (1991) 2687-2691
13. Liberles S.D., Diver S.T., Austin D.J., and Schreiber S.L. Inducible gene expression and protein translocation using nontoxic ligands identified by a mammalian three-hybrid screen. Proc. Natl. Acad. Sci. USA 94 (1997) 7825-7830
14. Lyman S.K., and Gerace L. Nuclear pore complexes: dynamics in unexpected places. J. Cell Biol. 154 (2001) 17-20
15. Marcus G.A., Horiuchi J., Silverman N., and Guarente L. ADA5/SPT20 links the ADA and SPT genes, which are involved in yeast transcription. Mol. Cell. Biol. 16 (1996) 3197-3205
16. Nasmyth K. How might cohesin hold sister chromatids together?. Philos. Trans. R. Soc. Lond. B Biol. Sci. 360 (2005) 483-496
17. Phair R.D., Scaffidi P., Elbi C., Vecerova J., Dey A., Ozato K., Brown D.T., Hager G., Bustin M., and Misteli T. Global nature of dynamic protein-chromatin interactions in vivo: three-dimensional genome scanning and dynamic interaction networks of chromatin proteins. Mol. Cell. Biol. 24 (2004) 6393-6402
18. Schmid M., Arib G., Laemmli C., Nishikawa J., Durussel T., and Laemmli U.K. Nup-PI: the nucleopore-promoter interaction of genes in yeast. Mol. Cell 21 (2006) 379-391
19. + RNA and nuclear pores. EMBO J. 16 (1997) 3256-3271
20. Spahn C.M., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., and Frank J. Structure of the 80S ribosome from Saccharomyces cerevisiae-tRNA-ribosome and subunit-subunit interactions. Cell 107 (2001) 373-386
21. Thompson C.M., Koleske A.J., Chao D.M., and Young R.A. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73 (1993) 1361-1375
22. Warner J.R. The economics of ribosome biosynthesis in yeast. Trends Biochem. Sci. 24 (1999) 437-440
23. Zemp I., and Kutay U. Nuclear export and cytoplasmic maturation of ribosomal subunits. FEBS Lett. 581 (2007) 2783-2793
24. Zhao K., Hart C.M., and Laemmli U.K. Visualization of chromosomal domains with boundary element-associated factor BEAF-32. Cell 81 (1995) 879-889