Insight into the coupling mechanism of the vitamin K-dependent carboxylase: Mutation of histidine 160 disrupts glutamic acid carbanion formation and efficient coupling of vitamin K epoxidation to glutamic acid carboxylation

Biochemistry

Volumn 47, Issue 37, 2008, Pages 9836-9846

  Rishavy, Mark A ^a   Berkner, Kathleen L ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; FOOD ADDITIVES; MECHANISMS; STOICHIOMETRY; TRITIUM;

CARBON POSITION; COUPLING EFFICIENCIES; COUPLING MECHANISMS; EFFICIENT COUPLING; GLUTAMIC ACID; HALF-REACTIONS; PROTON ABSTRACTION; TRITIUM RELEASE; VITAMIN K; WILD-TYPE ENZYME; WILD-TYPE LEVELS;

CARBOXYLATION;

ALANINE; CARBON; CARBON DIOXIDE; CARBOXYLASE; GLUTAMIC ACID; HISTIDINE; VITAMIN K GROUP;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CARBOXYLATION; CATALYSIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EPOXIDATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTON TRANSPORT; WILD TYPE;

ANIONS; CARBON DIOXIDE; CARBON-CARBON LIGASES; GLUTAMIC ACID; HISTIDINE; KINETICS; MUTATION; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; VITAMIN K;

BACTERIA (MICROORGANISMS); METAZOA;

EID: 51849094451     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800296r     Document Type: Article

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