Cytotoxicity and antibody binding by flow cytometry: A single assay to simultaneously assess two parameters

Cytometry Part B - Clinical Cytometry

Volumn 74, Issue 5, 2008, Pages 287-294

  Saw, Chee L ^a   Bray, Robert A ^a   Gebel, Howard M ^a  

^a EMORY UNIVERSITY HOSPITAL   (United States)

Author keywords

Crossmatching; Cytotoxicity; Flow cytometry; HLA antibodies; Transplant

Indexed keywords

COMPLEMENT; HLA ANTIBODY; HUMAN IMMUNOGLOBULIN;

ANTIBODY DETECTION; ANTIGEN BINDING; ARTICLE; CELL ASSAY; CELL DEATH; COMPLEMENT DEPENDENT CYTOTOXICITY; COMPLEMENT FIXATION; CONTROLLED STUDY; CYTOTOXICITY; DESENSITIZATION; FLOW CYTOMETRY; GRAFT RECIPIENT; HISTOCOMPATIBILITY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LABORATORY TEST; ORGAN TRANSPLANTATION; PATIENT CODING; PREOPERATIVE EVALUATION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; VALIDATION STUDY;

ANTIBODIES; ANTIBODY SPECIFICITY; COMPLEMENT FIXATION TESTS; CYTOTOXICITY TESTS, IMMUNOLOGIC; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENCE; GLOBULINS; HISTOCOMPATIBILITY TESTING; HUMANS; SERUM; T-LYMPHOCYTES;

EID: 51749100020     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20424     Document Type: Article

References (19)

1. Gebel HM, Bray RA, Nickerson P. Pre-transplant assessment of donor-feactive. HLA-specific antibodies in renal transplantation: Contraindication vs. risk. Am J Transplant 2003;3:1488-1500.
2. Pei R, Wang G, Tarsitani C, Rojo S, Chen T, Takemura S, Liu A, Lee JH. Simultaneous HLA class I and class II antibody screening with flow cytometry. Hum Immunol 1998;59:313-322.
3. Bray RA, Lebeck LK, Gebel HM. The flow cytometric crossmatch. Transplantation 1989;48:834-840.
4. Gloor JM, DeGoey S, Ploeger N, Gebel H, Bray R, Moore SB, Dean PG, Stegall MD. Persistence of low levels of alloantibody after desensitization in crossmatch-positive living-donor kidney transplantation. Transplantation 2004;78:221-227.
5. Patel R, Terasaki PI. Significance of the positive crossmatch test in kidney transplantation. N Engl J Med 1969;280:735-739.
6. Bray RA, Nickerson PW, Kerman RH, Gebel HM. Evolution of HLA antibody detection: Technology emulating biology. Immunol Res 2004;29:41-54.
7. Wahrmann M, Exner M, Regele H, Derfler K, Kormoczi GF, Lhotta K, Zlabinger GJ, Bohmig GA. Flow cytometry based detection of HLA alloantibody mediated classical complement activation. J Immunol Methods 2003;275:149-160.
8. Schonemann C, Lachmann N, Kiesewetter H, Salama A. Flow cytometric detection of complement-activating HLA antibodies. Cytometry B Clin Cytom 2004;62B:39-45.
9. Won DI, Jeong HD, Kim YL, Sub JH. Simultaneous detection of antibody binding and cytotoxicity in flow cytometry crossmatch for renal transplantation. Cytometry B Clin Cytom 2006;70B:82-90.
10. Pei R, Lee JH, Shih NJ, Chen M, Terasaki PI. Single human leukocyte antigen flow cytometry beads for accurate identification of human leukocyte antigen antibody specificities. Transplantation 2003;75: 43-49.
11. Bray RA, Nolen JD, Larsen C, Pearson T, Newell KA, Kokko K, Guasch A, Tso P, Mendel JB, Gebel HM. Transplanting the highly sensitized patient: The emory algorithm. Am J Transplant 2006;6: 2307-2315.
12. Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992;39: 225-235.
13. Fuller T, Phelan D, Gebel HM, Rodey G. Antigenic specificity of antibody reactive in the antiglobulin-augmented lymphocytotoxicity test. Transplantation 1982;34:24-29.
14. American Society for Histocompatibility and Immunogenetics.Standards for histocompatibility testing. Lenexa, KS:American Society for Histocompatibility and Immunogenetics, 1998.
15. Vaidya S, Cooper TY, Avandsalehi J, Barnes T, Brooks K, Hymel P, Noor M, Sellers R, Thomas A, Stewart D, Daller J, Fish JC, Gugliuzza KK, Bray RA. Improved flow cytometric detection of HLA alloantibodies using pronase: Potential implications in renal transplantation. Transplantation 2001;71:422-428.
16. Kerman RH, Van Buren CT, Lewis RM, DeVera V, Baghdahsarian V, Gerolami K, Kahan BD. Improved graft survival for flow cytometry and antihuman globulin crossmatch-negative retransplant recipients. Transplantation 1990;49:52-56.
17. Scornik JC, Brunson ME, Schaub B, Howard RJ, Pfaff WW. The cross-match in renal transplantation. Evaluation of flow cytometry as a replacement for standard cytotoxicity. Transplantation 1994;57:621-625.
18. Stefoni S, Nanni-Costa A, Buscaroli A, Borgnino LC, Iannelli S, Raimondi C, Scolari MP, Feliciangeli G, Bonomini V. Validity of flow cytometry for cross-match evaluation in clinical renal transplantation. Nephron 1991;57:268-272.
19. Bryan CF, McDonald SB, Luger AM, Shield CF, Winklhofer FT, Michael Borkon A, Warady BA, Aeder MI, Murillo D. Successful renal transplantation despite low levels of donor-specific HLA class I antibody without IVIg or plasmapheresis. Clin Transplant 2006;20:563-570.