Comparison of multiple gene assembly methods for metabolic engineering

Applied Biochemistry and Biotechnology

Volumn 137-140, Issue 1-12, 2007, Pages 703-710

  Lu, Chenfeng ^a   Mansoorabadi, Karen ^a   Jeffries, Thomas ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b FOREST PRODUCTS LABORATORY   (United States)

Author keywords

Cloning; Gene expression; Ligation; Optimization; Xylose; Yeast

Indexed keywords

CLONING; DNA; GENE EXPRESSION; GENETIC ENGINEERING; INDUSTRIAL RESEARCH; NUCLEIC ACIDS; ORGANIC ACIDS; POLYMERIZATION; PUMPS; SURGICAL EQUIPMENT; TECHNOLOGY; VECTORS;

6-PHOSPHOGLUCONATE DEHYDROGENASE; BIOLOGICAL RESEARCH; DNA ASSEMBLIES; DNA FRAGMENTS; DNA GLYCOSYLASE; GENE ASSEMBLY; LIGATION; METABOLIC ENGINEERING; MULTIPLE CLONING; OPTIMIZATION; OVERLAP EXTENSION POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; VECTOR CONSTRUCTION; XYLOSE; YEAST;

GENES;

DNA FRAGMENT; PHOSPHOGLUCONATE DEHYDROGENASE; URACIL DNA GLYCOSIDASE;

COMPARATIVE STUDY; CONFERENCE PAPER; ESCHERICHIA COLI; GENE; GENE CONSTRUCT; GENE EXPRESSION; METABOLIC ENGINEERING; MOLECULAR CLONING; MULTIPLE GENE ASSEMBLY; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; PYK1 GENE; RESTRICTION FRAGMENT; SACCHAROMYCES CEREVISIAE; TAL1 GENE; TKL1 GENE;

GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENETIC VECTORS; PLASMIDS; PROTEIN ENGINEERING; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 51349144785     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-007-9090-y     Document Type: Conference Paper

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