Identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, PCR-based restriction fragment length polymorphism analysis and species-specific PCR

Journal of Veterinary Medical Science

Volumn 70, Issue 8, 2008, Pages 837-840

  Ohya, Tatsuo ^a   Araki, Hiroshi ^b   Sueyoshi, Masuo ^c  

^a NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^b Nagasaki Prefecture Kenhoku Livestock Hygiene Service Center   (Japan)

^c UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Brachyspira; PCR RFLP; Spirochete

Indexed keywords

ANIMAL; ANIMAL DISEASE; ARTICLE; BRACHYSPIRA; CLASSIFICATION; GENETICS; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SWINE; SWINE DISEASE;

ANIMALS; BRACHYSPIRA; GRAM-NEGATIVE BACTERIAL INFECTIONS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SWINE; SWINE DISEASES;

BRACHYSPIRA; SPIROCHAETALES; SUIDAE; SUS;

EID: 51349093969     PISSN: 09167250     EISSN: 13477439     Source Type: Journal    
DOI: 10.1292/jvms.70.837     Document Type: Article

References (17)

1. Belanger, M. and Jacques, M. 1991. Evaluation of An-Ident system and an indole spot test for the rapid differentiation of porcine treponemes. J. Clin. Microbiol. 29: 1727-2729.
2. Fellström, C. and Gunnarsson, A. 1995. Phenotypical characterization of intestinal spirochaetes isolated from pigs. Res. Vet. Sci. 59: 1-4.
3. Fellström, C., Petterson, B., Thomson, J., Gunnarsson, A., Per-sson, M. and Johansson, K. E. 1997. Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. J. Clin. Microbiol. 35: 462-467.
4. Fellström, C., Karlsson, M., Petterson, B., Zimmerman, U., Gunnarsson, A. and Aspan, A. 1999. Emended description of indole negative and indole positive isolates of Brachyspira (Serpulina) hyodysenteriae. Vet. Microbiol. 70: 225-238.
5. Hampson, D. J. and Duhamel, G. E. 2006. Porcine colonic spirochetosis/intestinal spirochetosis. pp. 755-767. In: Diseases of Swine, 9th ed. (Straw, B. E., Zimmerman, J. J., D'Allaire, D. and Taylor, D. J. eds.), Iowa State University, Ames.
6. Hampson, D. J., Fellström, C. and Thompson, J. R. 2006. Swine dysentery. pp. 785-805. In: Diseases of Swine, 9th ed. (Straw, B. E., Zimmerman, J. J., D'Allaire, D. and Taylor, D. J. eds.), Iowa State University, Ames.
7. Hwang, M. and Ederer, G M. 1975. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J. Clin. Microbiol. 1: 114-115.
8. Kinyon, J. M. and Harris, D. L. 1979. Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae. Int. J. Syst. Bacterial. 29: 102-109.
9. Lee,J. I., Hampson, D. J., Lymbery, A. J. and Harders, S. J. 1993. The porcine intestinal spirochaetes: identification of new genetic groups. Vet. Microbiol. 34: 273-285.
10. Neef, N. A., Lysons, R. J., Trott, D. J., Hampson, D. J., Jones, P. W. and Morgan, J. H. 1994. Pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. Infect. Immun. 62: 2395-2403.
11. Rohde, J., Rothkamp, A. and Gerlach, G. 2002. Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis. J. Clin. Microbiol. 40: 2598-2600.
12. Stanton, T. B., Fournie-Amazouz, E., Postic, D., Trott, D. J., Grimont, P. A. D., Baranton, D., Hampson, D. J. and Saint Girons, I. 1997. Identification of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. Int. J. Syst .Bacteriol. 47: 1007-1012.
13. Sutter, V. L. and Carter, W. T. 1972. Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology. Am. J. Clin. Pathol. 58: 335-338.
14. Taylor, D. J., Simmons, J. R and Laird, H. M. 1980. Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. Vet. Rec. 106: 326-332.
15. Trott, D., Stanton, T. B., Jensen, N. S., Duhamel, G. E., Johnson, J. L. and Hampson, D. J. 1996. Serpuiina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int. J. Syst. Bacteriol. 46: 206-215.
16. Weissenböck, H., Maderner, A., Herzog, A. M., Lussy, H. and Nowotny, N. 2005. Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspira murdochii. Vet. Microbiol. 111: 67-75.
17. Wilcock, B. P. and Olander, H. J. 1979. Characterization of the lesions in colons and colonic segments inoculated with pure cultures or colonic content containing Treponema hyodysenteriae. Vet. Pathol. 16: 450-465.