A rapid procedure for the construction of PCR cDNA libraries from small amounts of plant tissue

Plant Molecular Biology Reporter

Volumn 9, Issue 2, 1991, Pages 131-138

  Jepson, Ian ^a   Bray, John ^b   Jenkins, Gareth ^b   Schuch, Wolfgang ^a   Edwards, Keith ^a  

^a JEALOTT S HILL RESEARCH STATION   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Beta vulgaris; cDNA library; floral apices; PCR amplification; RNA extraction

Indexed keywords

EID: 51249170782     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/BF02669206     Document Type: Article

References (8)

1. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells
2. cDNA library construction from small amounts of unfractionated RNA: Association of cDNA synthesis with polymerase chain reaction amplification
3. A simple method for subclonign DNA fragments from gel slices
4. Construction and screening cDNA libraries in λgt10 and λgt11
5. High-efficiency cloning of full-length cDNA
6. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
7. Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells