Expression and purification of non-glycosylated Trypanosoma brucei transferrin receptor in insect cells

Experimental Parasitology

Volumn 120, Issue 2, 2008, Pages 205-207

  Maier, Alexander ^a   Steverding, Dietmar ^a,b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

ESAG; expression site associated gene; Glycosylation; glycosylphosphatidylinositol; GPI; Insect cells; N Glycosidase F; PNGase F; Transferrin receptor; Trypanosoma brucei; Tunicamycin

Indexed keywords

GENE PRODUCT; GLYCOSYLPHOSPHATIDYLINOSITOL; PROTEIN ESAG6; PROTEIN ESAG7; RECOMBINANT PROTEIN; TRANSFERRIN; TRANSFERRIN RECEPTOR; TUNICAMYCIN; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; DIMERIZATION; GENE EXPRESSION; GLYCOSYLATION; INSECT CELL; LIGAND BINDING; MAST CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; TRYPANOSOMA BRUCEI;

ANIMALS; BACULOVIRIDAE; CELL LINE; GENE EXPRESSION; GLYCOPROTEINS; GLYCOSYLATION; IMMUNOBLOTTING; PROTOZOAN PROTEINS; RECEPTORS, TRANSFERRIN; SPODOPTERA; TRYPANOSOMA BRUCEI BRUCEI; TUNICAMYCIN;

HEXAPODA; TRYPANOSOMA BRUCEI;

EID: 51249123730     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2008.07.004     Document Type: Article

References (18)

1. Biebinger S., Helfert S., Steverding D., Ansorge I., and Clayton C. Impaired dimerization and trafficking of ESAG6 lacking a glycosyl-phosphatidylinositol anchor. Molecular and Biochemical Parasitology 132 (2003) 93-96
2. Breidbach T., Scory S., Krauth-Siegel R.L., and Steverding D. Growth inhibition of bloodstream forms of Trypanosoma brucei by the iron chelator deferoxamine. International Journal for Parasitology 32 (2002) 473-479
3. Chaudhri M., Steverding D., Kittelberger D., Tjia S., and Overath P. Expression of a glycosylphosphatidylinositol-anchored Trypanosoma brucei transferrin-binding protein complex in insect cells. Proceedings of the National Academy of Science USA 91 (1994) 6443-6447
4. Hunt R.C., Riegler R., and Davis A.A. Changes in glycosylation alter the affinity of the human transferrin receptor for its ligand. Journal of Biological Chemistry 264 (1989) 9643-9648
5. Ligtenberg M.J.L., Bitter W., Kieft R., Steverding D., Janssen H., Calafat J., and Borst P. Reconstitution of a surface transferrin binding complex in insect form Trypanosoma brucei. EMBO Journal 13 (1994) 2565-2573
6. Merschjohann K., and Steverding D. In vitro growth inhibition of bloodstream forms of Trypanosoma brucei and Trypanosoma congolense by iron chelators. Kinetoplastid Biology and Disease 5 (2006) 3
7. Olson T.S., and Lane M.D. Post-translational acquisition of insulin binding activity by the insulin proreceptor. Correlation to recognition by autoimmune antibody. Journal of Biological Chemistry 262 (1987) 6816-6822
8. Pays E., Tebabi P., Pays A., Coquelet H., Revelard P., Salmon D., and Steinert M. The genes and transcripts of an antigen gene expression site from T. brucei. Cell 57 (1989) 835-845
9. Salmon D., Geuskens M., Hanocq F., Hanocq-Quertier J., Nolan D., Ruben L., and Pays E. A novel heterodimeric transferrin receptor encoded by a pair of VSG expression site-associated genes in T. brucei. Cell 78 (1994) 75-86
10. Salmon D., Hanocq-Quertier J., Paturiaux-Hanocq F., Pays A., Tebabi P., Nolan D.P., Michel A., and Pays E. Characterization of the ligand-binding site of the transferrin receptor in Trypanosoma brucei demonstrates a structural relationship with the N-terminal domain of the variant surface glycoprotein. EMBO Journal 16 (1997) 7272-7278
11. Schell D., Borowy N.K., and Overath P. Transferrin is a growth factor for the bloodstream form of Trypanosoma brucei. Parasitology Research 77 (1991) 558-560
12. Slieker L.J., Martensen T.M., and Lane M.D. Synthesis of epidermal growth factor receptor in human A431 cells. Glycosylation-dependent acquisition of ligand binding activity occurs post-translationally in the endoplasmic reticulum. Journal of Biological Chemistry 261 (1986) 15233-15241
13. Steverding D. Bloodstream forms of Trypanosoma brucei require only small amounts of iron for growth. Parasitology Research 84 (1998) 59-62
14. Steverding D. The transferrin receptor of Trypanosoma brucei. Parasitology International 48 (2000) 191-198
15. Steverding D., Stierhof Y.-D., Chaudhri M., Ligtenberg M., Schell D., Beck-Sickinger A.G., and Overath P. ESAG6 and 7 products of Trypanosoma brucei form a transferrin binding protein complex. European Journal of Cell Biology 64 (1994) 78-87
16. Steverding D., Stierhof Y.-D., Fuchs H., Tauber R., and Overath P. Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei. Journal of Cell Biology 131 (1995) 1173-1182
17. Trombetta E.S. The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis. Glycobiology 13 (2003) 77R-91R
18. Tsuji S., Kato H., Matsuoka Y., and Fukushima T. Molecular weight heterogeneity of bovine serum transferrin. Biochemical Genetics 22 (1984) 1145-1159