Segmentation of brain immunohistochemistry images using clustering of linear centroids and regional shapes

Journal of Imaging Science and Technology

Volumn 52, Issue 4, 2008, Pages 0405021-04050211

  Wu, Hai Shan ^a   Murray, Jacinta ^a   Morgello, Susan ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTO COVARIANCES; CELL NUCLEUS; GEOMETRICAL SHAPES;

APPROXIMATION ALGORITHMS; BOOLEAN FUNCTIONS; CELLS; CHLORINE COMPOUNDS; CLUSTERING ALGORITHMS; COPYING; COVARIANCE MATRIX; CYTOLOGY; FLOW OF SOLIDS; IMAGE ENHANCEMENT; ITERATIVE METHODS; PAINTING; PIXELS;

VECTORS;

EID: 51049103148     PISSN: 10623701     EISSN: None     Source Type: Journal    
DOI: 10.2352/J.ImagingSci.Technol.(2008)52:4(040502)     Document Type: Article

References (16)

1. L. G. Alexopoulos, G. R. Erickson, and F. Guilak, "A method for quantifying cell size from differential interference contrast images: Validation and application to osmotically stressed chondrocytes", I. Microsc. 205, 125-135 (2002).
2. X. Chen, X. Zhou, and S. T. Wong, "Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy", IEEE Trans. Biomed. Eng. 53, 762-766 (2006).
3. H.-S. Wu, J. Barba, and J. Gil, "A parametric fitting algorithm for segmentation of cell images", IEEE Trans. Biomed. Eng. 45, 400-407 (1998).
4. H.-S. Wu, J. Barba, and J. Gil, "Iterative thresholding for segmentation of cell images", J. Microsc. 197, 296-304 (2000).
5. H.-S. Wu, R. Xu, N. Harpaz, D. Burstein, and J. Gil, "Segmentation of intestinal gland images with iterative region growing", J. Microsc. 220, 190-204 (2005).
6. L. D. Biertho, C. Kim, H.-S. Wu, P. Unger, and W. B. Inabnet, "Relationship between sestamibi uptake, parathyroid hormone assay and nuclear morphology in primary hyperparathyroidism", J. Am. Coll. Surg. 199, 229-233 (2004).
7. L. Deligdisch, C. Miranda, H.-S. Wu, and J. Gil, "Human papillomavirus-related cervical lesions in adolescents: A histologic and morphometric study", Gynecol. Oncol. 89, 52-59 (2003).
8. T. Mattfeldt, H.-W. Gottfried, V. Schmidt, and H. A., Kestler, "Classification of spatial textures in benign and cancerous glandular tissues by stereology and stochastic geometry using artificial neural networks", J. Microsc. 198, 143-158 (2000).
9. C. O. D. Solorzano, E. G. Rodriguez, A. Jones, D. Pinkel, J. W., Gray, D. Sudar, and S. J. Lockett, "Segmentation of confocal microscope images of cell nuclei in thick tissue sections", J. Microsc. 193, 212-226 (1999).
10. T. Hortobagyi, S. Wise, N. Hunt, N. Cary, V. Djurovic, A. Fegan-Earl, K. Shorrock, D. Rouse, and S. Al-Sarraj, "Traumatic axonal damage in the brain can be detected using β-APP immunohistochemistry within 35 min after head injury to human adults", Neuropathol. Appl. Neurobiol. 33, 226-237 (2007).
11. L. D'Este, A. Casini, S. Puglisi-Allegra, S. Cabib, and T. G. Renda, "Comparative immunohistochemical study of the dopaminergic systems in two inbred mouse strains (C57BL/6J and DBA/2J)", J. Chem. Neuroanat. 33, 67-74 (2007).
12. A. -L. Mausset-Bonnefont, R. D. Seze, and A. Privat, " Immunohistochemistry as a tool for topographical semi-quantification of neurotransmitters in the brain", Brain Res. Brain Res. Protoc 10, 148-155 (2003).
13. W. T. Shaia, S. M. Shapiro, A. J. Heller, D. I., Galiani, A. Sismanis, and R. F. Spencer, "Immunohistochemical localization of calcium-binding proteins in the brainstem vestibular nuclei of the jaundiced Gunn rat", Hear. Res. 173, 82-90 (2002).
14. H.-S. Wu and J. Gil, "Linear clustering for segmentation of color microscopic lung cell images", J. Imaging Sci. Technol. 47, 161-170 (2003).
15. R. J. Schalkoff, Digital Image Processing and Computer Vision (Wiley, New York, 1989).
16. A. C. Ruifrok and D. A. Johnston, "Quantification of histochemical staining by color dcconvolution", Anal Quant Cytol. Histol. 23, 291-299 (2001).