메뉴 건너뛰기




Volumn 5, Issue 3, 2008, Pages 148-155

Cloning, expression and purification of truncated Chlamydia Trachomatis outer membrane protein 2 (Omp2) and its application in an ELISA assay

Author keywords

Chlamydia trachomatis; ELISA; Omp2; Recombinant protein

Indexed keywords

ANTIBODY; BACTERIAL PROTEIN; OUTER MEMBRANE PROTEIN 2; POLYSTYRENE; UNCLASSIFIED DRUG;

EID: 50949121125     PISSN: 17351383     EISSN: 1735367X     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (5)

References (25)
  • 1
    • 50949086303 scopus 로고    scopus 로고
    • Topley and Wilson's Microbiology and Microbial Infections
    • 9th edition
    • Collier L, Balows A, Sussman M. Topley and Wilson's Microbiology and Microbial Infections: Vol 3: Bacterial Infections. 9th edition, 1998; pp. 977-91.
    • (1998) Bacterial Infections , vol.3 , pp. 977-991
    • Collier, L.1    Balows, A.2    Sussman, M.3
  • 2
    • 0024572661 scopus 로고
    • Identification by Sequence Analysis of Two-Site Posttranslational Processing of the Cysteine-Rich Outer Membrane Protein 2 of Chlamydia trachomatis Serovar L2
    • Allen JE, Stephens RS. Identification by Sequence Analysis of Two-Site Posttranslational Processing of the Cysteine-Rich Outer Membrane Protein 2 of Chlamydia trachomatis Serovar L2. J Bacteriol. 1989; 17: 285-91.
    • (1989) J Bacteriol , vol.17 , pp. 285-291
    • Allen, J.E.1    Stephens, R.S.2
  • 3
    • 0024999670 scopus 로고
    • The nucleotide and derived amino acid sequence of the omp2 gene of Chlamydia trachomatis serovar E
    • Coles AM, Allan I, Pearce JH. The nucleotide and derived amino acid sequence of the omp2 gene of Chlamydia trachomatis serovar E. Nucleic Acids Res. 1990; 18: 6713.
    • (1990) Nucleic Acids Res , vol.18 , pp. 6713
    • Coles, A.M.1    Allan, I.2    Pearce, J.H.3
  • 4
    • 0034759451 scopus 로고    scopus 로고
    • Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens
    • Bas S, Muzzin P, Vischer TL. Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens. J Clin Microbiol. 2001; 39: 4082-5.
    • (2001) J Clin Microbiol , vol.39 , pp. 4082-4085
    • Bas, S.1    Muzzin, P.2    Vischer, T.L.3
  • 5
    • 0035060526 scopus 로고    scopus 로고
    • Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens
    • Bas S, Muzzin P, Ninet B, Bornand JE, Scieux C, Vischer TL. Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens. J Clin Microbiol. 2001; 39: 1368-7.
    • (2001) J Clin Microbiol , vol.39 , pp. 1368-1377
    • Bas, S.1    Muzzin, P.2    Ninet, B.3    Bornand, J.E.4    Scieux, C.5    Vischer, T.L.6
  • 6
    • 0034905031 scopus 로고    scopus 로고
    • Indirect evidence of intra-articular immunoglobulin G synthesis in patients with Chlamydia trachomatis reactive arthritis
    • Bas S, Muzzin P, Fulpius T, Buchs N, Vischer TL. Indirect evidence of intra-articular immunoglobulin G synthesis in patients with Chlamydia trachomatis reactive arthritis. Rheumatology. 2001; 40: 801-5.
    • (2001) Rheumatology , vol.40 , pp. 801-805
    • Bas, S.1    Muzzin, P.2    Fulpius, T.3    Buchs, N.4    Vischer, T.L.5
  • 7
    • 0037250366 scopus 로고    scopus 로고
    • Characterization of the Humoral Immune Response to Chlamydia Outer Membrane Protein 2 in Chlamydial Infection
    • Portig I, Goodal JC, Bailey RL, Gaston JS. Characterization of the Humoral Immune Response to Chlamydia Outer Membrane Protein 2 in Chlamydial Infection. Clin Diagn Lab Immunol. 2003; 10: 103-7.
    • (2003) Clin Diagn Lab Immunol , vol.10 , pp. 103-107
    • Portig, I.1    Goodal, J.C.2    Bailey, R.L.3    Gaston, J.S.4
  • 8
    • 0036735230 scopus 로고    scopus 로고
    • Importance of species-specific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis
    • Bas S, Genevay S, Schenkel MC, Vischer TL. Importance of species-specific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis. Rheumatology. 2002; 41: 1017-20.
    • (2002) Rheumatology , vol.41 , pp. 1017-1020
    • Bas, S.1    Genevay, S.2    Schenkel, M.C.3    Vischer, T.L.4
  • 9
    • 34247202138 scopus 로고    scopus 로고
    • Distribution study of Chlamydia trachomatis serovars among high-risk women in China performed using PCR-restriction fragment length polymorphism genotyping
    • Gao X, Chen XS, Yin YP, Zhoang MY, Shi MQ, Wei WH et al. Distribution study of Chlamydia trachomatis serovars among high-risk women in China performed using PCR-restriction fragment length polymorphism genotyping. J Clin Microbiol. 2007;45:1185-9.
    • (2007) J Clin Microbiol , vol.45 , pp. 1185-1189
    • Gao, X.1    Chen, X.S.2    Yin, Y.P.3    Zhoang, M.Y.4    Shi, M.Q.5    Wei, W.H.6
  • 11
    • 0003391916 scopus 로고    scopus 로고
    • PCR, the Basics from Background to Bench. Bios Scientific publishers
    • Chapter 2
    • Pherson Mc, Moller MJ. PCR, the Basics from Background to Bench. Bios Scientific publishers. Chapter 2, Undrestanding PCR. 2000; pp: 9-21.
    • (2000) Undrestanding PCR , pp. 9-21
    • Pherson, M.1    Moller, M.J.2
  • 12
    • 9444264709 scopus 로고
    • The extraction and isolation of DNA from gels, in Molecular Biology. Nucleic Acids. John M, edotor
    • Gaastra W, Jorgensen PL. The extraction and isolation of DNA from gels, in Molecular Biology. Nucleic Acids. John M, edotor. Walker. Chapter 10, 1984; pp: 67-76.
    • (1984) Walker. Chapter , vol.10 , pp. 67-76
    • Gaastra, W.1    Jorgensen, P.L.2
  • 13
    • 84898706644 scopus 로고    scopus 로고
    • Eun HM. Enzymology primer for Recombinant DNA Technology. Academic Press. Chapter 6, DNA polymerases, 1996: pp. 345-489.
    • Eun HM. Enzymology primer for Recombinant DNA Technology. Academic Press. Chapter 6, DNA polymerases, 1996: pp. 345-489.
  • 14
    • 84898711552 scopus 로고    scopus 로고
    • Gaastra W, Klemm P. Radiolabeling of DNA with 3′ terminal transferase. In: Molecular Biology. Nucleic Acids. Walker JM, editor. Chapter 40; 1984: pp. 269-271.
    • Gaastra W, Klemm P. Radiolabeling of DNA with 3′ terminal transferase. In: Molecular Biology. Nucleic Acids. Walker JM, editor. Chapter 40; 1984: pp. 269-271.
  • 15
    • 84898709659 scopus 로고    scopus 로고
    • Gaastra W, Hansen K. Ligation of DNA with T4 DNA ligase. In: Methods in Molecular Biology. Nucleic Acids. Walker JM, editor. Humana press. Chapter 32, 1984; pp: 225-30.
    • Gaastra W, Hansen K. Ligation of DNA with T4 DNA ligase. In: Methods in Molecular Biology. Nucleic Acids. Walker JM, editor. Humana press. Chapter 32, 1984; pp: 225-30.
  • 16
    • 0343404578 scopus 로고
    • Studies on transformation of Escherichia coli with plasmids
    • Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 98: 503-17.
    • (1983) J Mol Biol , vol.98 , pp. 503-517
    • Hanahan, D.1
  • 17
    • 0027248058 scopus 로고
    • A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli
    • Feliciello I, Chinali G. A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem. 1993; 212: 394-401.
    • (1993) Anal Biochem , vol.212 , pp. 394-401
    • Feliciello, I.1    Chinali, G.2
  • 18
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-54.
    • (1976) Anal Biochem , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 19
    • 41149179977 scopus 로고    scopus 로고
    • Protein blotting, pricipales and application
    • Handbook, Rapley R, Walker JM, editors. chapter 34, 1998; pp
    • Shewry PR, Fido RJ. Protein blotting, pricipales and application. In: Molecular Biomethods Handbook, Rapley R, Walker JM, editors. chapter 34, 1998; pp. 435-44.
    • Molecular Biomethods , pp. 435-444
    • Shewry, P.R.1    Fido, R.J.2
  • 20
    • 0037107599 scopus 로고    scopus 로고
    • Covalent immobilization of proteins onto photoactivated polystyrene microliter plates for enzyme-linked immunosorbent assay procedures
    • Bora U, Chugh L, Nahar P. Covalent immobilization of proteins onto photoactivated polystyrene microliter plates for enzyme-linked immunosorbent assay procedures. J Immunol Methods. 2002; 268: 171-7.
    • (2002) J Immunol Methods , vol.268 , pp. 171-177
    • Bora, U.1    Chugh, L.2    Nahar, P.3
  • 21
    • 0002465481 scopus 로고
    • Urethritis in males
    • Holmes K K, Mardh PA, Sparling PF, Wiesner PJ:, 2nd ed. McGraw-Hill, New York;, PP
    • nd ed. McGraw-Hill, New York; 1989: PP. 627-39.
    • (1989) Sexually transmitted disease , pp. 627-639
    • Bowie, W.R.1
  • 22
    • 0025601863 scopus 로고
    • Epidemiology of sexually transmitted disease: The globol picture
    • De Schryver A, Meheus A. Epidemiology of sexually transmitted disease: the globol picture. Bull. WHO. 1990; 68: 639-54.
    • (1990) Bull. WHO , vol.68 , pp. 639-654
    • De Schryver, A.1    Meheus, A.2
  • 23
    • 0034672107 scopus 로고    scopus 로고
    • Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
    • Kim SK, Devine L, Angevine M, DeMars R, Kavathas PB. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. J Immunol. 2000; 165: 7285-92.
    • (2000) J Immunol , vol.165 , pp. 7285-7292
    • Kim, S.K.1    Devine, L.2    Angevine, M.3    DeMars, R.4    Kavathas, P.B.5
  • 24
    • 0035657276 scopus 로고    scopus 로고
    • Recognition of the 60 kilodalton cysteine-rich outer membrane protein OMP2 by CD4 (+) T cells from humans infected with Chlamydia trachomatis
    • Goodall JC, Beacock- Sharp H, Deane KH, Gaston JS. Recognition of the 60 kilodalton cysteine-rich outer membrane protein OMP2 by CD4 (+) T cells from humans infected with Chlamydia trachomatis. Clin Exp Immunol. 2001; 126: 488-93.
    • (2001) Clin Exp Immunol , vol.126 , pp. 488-493
    • Goodall, J.C.1    Beacock- Sharp, H.2    Deane, K.H.3    Gaston, J.S.4
  • 25
    • 0026080177 scopus 로고
    • Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene
    • Watson MW, Lambden PR, Clarke IN. Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. J Clin Microbiol. 1991; 29: 1183-93.
    • (1991) J Clin Microbiol , vol.29 , pp. 1183-1193
    • Watson, M.W.1    Lambden, P.R.2    Clarke, I.N.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.