Comet assay analysis of single-stranded DNA breaks in circulating leukocytes of glaucoma patients

Molecular Vision

Volumn 14, Issue , 2008, Pages 1584-1588

  Mozaffarieh, M ^a   Schoetzau, A ^a   Sauter, M ^a   Grieshaber, M ^a   Orgul S ^a   Golubnitschaja, O ^b   Flammer, Josef ^a,c  

^a UNIVERSITY EYE CLINIC   (Switzerland)

^b Experimentelle Radiologie und Strahlenbiologie   (Germany)

^c UNIVERSITY HOSPITAL BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ADULT; AGED; ARTICLE; CELL ISOLATION; CELL NUCLEUS; CELL SUSPENSION; CLINICAL ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; FEMALE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LEUKOCYTE; MALE; OPEN ANGLE GLAUCOMA; PRIORITY JOURNAL; SINGLE STRANDED DNA BREAK;

CELL MOVEMENT; COMET ASSAY; DEMOGRAPHY; DNA BREAKS, SINGLE-STRANDED; FEMALE; GLAUCOMA; HUMANS; LEUKOCYTES; MALE; MIDDLE AGED;

EID: 50949105076     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Martin LJ. DNA damage and repair: relevance to mechanisms of neurodegeneration. J Neuropathol Exp Neurol 2008; 67:377-87.
2. Katyal S, McKinnon PJ. DNA strand breaks, neurodegeneration and aging in the brain. Mech Ageing Dev 2008; 129:483-91.
3. SimoneSGorinYVelagapudiCAbboudHEHabibSLMechanism of Oxidative DNA damage in diabetes: tuberin inactivation and downregulation of DNA repair enzyme OGG1.Diabetes2008[Epub ahead of print]
4. Rothkamm K, Gunasekara K, Warda SA, Krempler A, Lobrich M. Radiation-induced HPRT mutations resulting from misrejoined DNA double-strand breaks. Radiat Res 2008; 169:639-48.
5. Seo KY, Jelinsky SA, Loechler EL. Factors that influence the mutagenic patterns of DNA adducts from chemical carcinogens. Mutat Res 2000; 463:215-46.
6. Aiub CA, Pinto LF, Felzenszwalb I. DNA-repair genes and vitamin E in the prevention of N-nitrosodiethylamine mutagenicity. Cell Biol Toxicol. 2008
7. Bartsch H, Nair J. Chronic inflammation and oxidative stress in the genesis and perpetuation of cancer: role of lipid peroxidation, DNA damage, and repair. Langenbecks Arch Surg 2006; 391:499-510.
8. Sacca SC, Pascotto A, Camicione P, Capris P, Izzotti A. Oxidative DNA damage in the human trabecular meshwork: clinical correlation in patients with primary open-angle glaucoma. Arch Ophthalmol 2605; 123:458-63.
9. Zhou L, Li Y, Yue BY. Oxidative stress affects cytoskeletal structure and cell-matrix interactions in cells from an ocular tissue: the trabecular meshwork. J Cell Physiol 1999; 180:182-9.
10. Moenkemann H, Flammer J,Wunderlich K, Breipohl W, Schild HH, Golubnitschaja O. Increased DNA breaks and up-regulation of both G(1) and G(2) checkpoint genes p21(WAF1/CIP1) and 14-3-3 sigma in circulating leukocytes of glaucoma patients and vasospastic individuals. Amino Acids 2005; 28:199-205.
11. DhawanABajpayeeMParmarDComet assay: a reliable tool for the assessment of DNA damage in different models.Cell Biol Toxicol2008[Epub ahead of print]
12. Choy CK, Benzie IF, Cho P. UV-mediated DNA strand breaks in corneal epithelial cells assessed using the comet assay procedure. Photochem Photobiol 2005; 81:493-7.
13. Davidkova M, Juha L, Bittner M, Koptyaev S, Hajkova V, Krasa J, Pfeifer M, Stisova V, Bartnik A, Fiedorowicz H, Mikolajczyk J, Ryc L, Pina L, Horvath M, Babankova D, Cihelka J, Civis S. A high-power laser-driven source of sub-nanosecond soft X-ray pulses for single-shot radiobiology experiments. Radiat Res 2007; 168:382-7.
14. Stap J, Krawczyk PM, Van Oven CH, Barendsen GW, Essers J, Kanaar R, Aten JA. Induction of linear tracks of DNA double-strand breaks by alpha-particle irradiation of cells. Nat Methods 2008; 5:261-6.
15. Arbillaga L, Azqueta A, Ezpeleta O. Lopez de CA. Oxidative DNA damage induced by Ochratoxin A in the HK-2 human kidney cell line: evidence of the relationship with cytotoxicity. Mutagenesis 2007; 22:35-42.
16. Chye SM, Hseu YC, Liang SH, Chen CH, Chen SC. Single strand dna breaks in human lymphocytes exposed to paraphenylenediamine and its derivatives. Bull Environ Contam Toxicol 2008; 80:58-62.
17. Li L, Jiang L, Geng C, Cao J, Zhong L. The role of oxidative stress in acrolein-induced DNA damage in HepG2 cells. Free Radic Res 2008; 42:354-61.
18. Mozaffarieh M, Flammer J. Is there more to glaucoma treatment than lowering IOP? Surv Ophthalmol 2007; 52:S174-9.
19. Flammer J. Glaucomatous optic neuropathy: a reperfusion injury. Klin Monatsbl Augenheilkd 2001;218:290-1
20. Flammer J, Mozaffirieh M. What is the present pathogenetic concept of glaucomatous optic neuropathy? Surv Ophthalmol 2007; 52:S162-73.
21. Flammer J, Mozaffarieh M. Autoregulation, a balancing act between supply and demand. Can J Ophthalmol 2008; 43:317-21.
22. Flammer J, Orgul S, Costa VP, Orzalesi N, Krieglstein GK, Serra LM, Renard JP, Stefansson E. The impact of ocular blood flow in glaucoma. Prog Retin Eye Res 2002; 21:359-93.
23. Abu-Amero KK, Morales J, Bosley TM. Mitochondrial abnormalities in patients with primary open-angle glaucoma. Invest Ophthalmol Vis Sci 2006; 47:2533-41.
24. Ferreira SM, Lerner SF, Brunzini R, Evelson PA, Llesuy SF. Oxidative stress markers in aqueous humor of glaucoma patients. Am J Ophthalmol 2004; 137:62-9.
25. Tamm ER, Russell P, Piatigorsky J. Development of characterization of a immortal and differentiated murine trabecular meshwork cell line. Invest Ophthalmol Vis Sci 1999; 40:1392-403.
26. Tezel G. Oxidative stress in glaucomatous neurodegeneration: mechanisms and consequences. Prog Retin Eye Res 2006; 25:490-513.
27. Yildirim O, Ates NA, Ercan B, Muslu N, Unlu A, Tamer L, Atik U, Kanik A. Role of oxidative stress enzymes in open-angle glaucoma. Eye 2005; 19:580-3.
28. Izzotti A, Sacca SC, Cartiglia C, De FS. Oxidative deoxyribonucleic acid damage in the eyes of glaucoma patients. Am J Med 2003; 114:638-46.
29. Gherghel D, Griffiths HR, Hilton EJ, Cunliffe IA, Hosking SL. Systemic reduction in glutathione levels occurs in patients with primary open-angle glaucoma. Invest Ophthalmol Vis Sci 2005; 46:877-83.
30. Yang J, Tezel G, Patil RV, Romano C, Wax MB. Serum autoantibody against glutathione S-transferase in patients with glaucoma. Invest Ophthalmol Vis Sci 2001; 42:1273-6.
31. Golubnitschaja-Labudova O, Liu R, Decker C, Zhu P, Haefliger IO, Flammer J. Altered gene expression in lymphocytes of patients with normal-tension glaucoma. Curr Eye Res 2000; 21:867-76.
32. Golubnitschaja O, Yeghiazaryan K, Liu R, Monkemann H, Leppert D, Schild H, Haefliger IO, Flammer J. Increased expression of matrix metalloproteinases in mononuclear blood cells of normal-tension glaucoma patients. J Glaucoma 2004; 13:66-72.
33. Wunderlich K, Golubnitschaja O, Pache M, Eberle AN, Flammer J. Increased plasma levels of 20S proteasome alpha-subunit in glaucoma patients: an observational pilot study. Mol Vis 2002; 8:431-5.
34. Golubnitschaja O, Yeghiazaryan K, Wunderlich K, Schild HH, Flammer J. Disease proteomics reveals altered gene expression regulation in leukocytes of normal-tension and primary open-angle glaucoma patients. Proteomics Clin Appl 2007; 1:1316-23.