A simple and quick method of directly observing growth and proliferation of composite skin cultured ex vivo

Burns

Volumn 34, Issue 7, 2008, Pages 1008-1010

  Xiao, Shichu ^a   Zhu, Shihui ^a   Ma, Bing ^a   Yang, Jun ^a   Xia, Zhaofan ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Composite skin; Dermal substitute; Haematoxylin eosin staining; Hyalinisation

Indexed keywords

EOSIN; HEMATOXYLIN;

ARTICLE; CELL GROWTH; CELL PROLIFERATION; CELL SURVIVAL; HYALINE DEGENERATION; KERATINOCYTE; PHASE CONTRAST MICROSCOPE; SKIN CULTURE;

ANIMALS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIVISION; FIBROBLASTS; HUMANS; KERATINOCYTES; MICROSCOPY, ELECTRON, SCANNING; SKIN, ARTIFICIAL; STATISTICS, NONPARAMETRIC; SWINE; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 50949088706     PISSN: 03054179     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.burns.2008.01.024     Document Type: Article

References (7)

1. Matouskova E., Broz L., Stolbova V., Klein L., Konigova R., and Vesely P. Human allogeneic keratinocytes cultured on acellular xenodermis: the use in healing of burns and other skin defects. Biomed Mater Eng 16 (2006) S63-S71
2. Price R.D., Das-Guptaf V., Frame J.D., and Navsaria H.A. A study to evaluate primary dressings for the application of cultured keratinocytes. Br J Plast Surg 54 (2001) 687-696
3. Walter R.J., Matsuda T., Reyes H.M., Walter J.M., and Hanumadass M. Characterization of acellular dermal matrices (ADMs) prepared by two different methods. Burns 24 (1998) 104-111
4. Erdag G., and Sheridan R.L. Fibroblasts improve performance of cultured composite skin substitutes on athymic mice. Burns 30 (2004) 322-328
5. Hu K., Dai Y., Hu Q., Li J., Yuan J., Li J., et al. An experimental study on the repair of full skin loss of nude mice with composite graft of epidermal stem cells. Burns 32 (2006) 416-422
6. Falkeholm L., Grant C.A., Magnusson A., and Möller E. Xylene-free method for histological preparation: a multicentre evaluation. Lab Invest 81 (2001) 1213-1221
7. Wishe H.I., Roy M., and Piliero S.J. A new organic solvent for use in the clearing of tissues I. Soft tissue histology. Anat Rec 197 (1980) 283-288