Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease of Natrialba magadii (Nep)

Extremophiles

Volumn 12, Issue 5, 2008, Pages 677-687

  De Castro, Rosana E ^a   Ruiz, Diego M ^a   Giménez, María I ^a   Silveyra, María X ^b   Paggi, Roberto A ^a   Maupin Furlow, Julie A ^c  

^a UNIVERSIDAD NACIONAL DE MAR DEL PLATA   (Argentina)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^c UNIVERSITY OF FLORIDA   (United States)

Author keywords

Gene cloning and expression; Haloalkaliphilic protease; Natrialba magadii; Solvent tolerance; Tat pathway

Indexed keywords

ARCHAEAL DNA; ARCHAEAL PROTEIN; PEPTIDE HYDROLASE; PRIMER DNA; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; ENZYMOLOGY; GENETICS; HALOBACTERIUM; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA PRIMERS; DNA, ARCHAEAL; HALOBACTERIACEAE; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; PLASMIDS; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; SERINE ENDOPEPTIDASES;

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HALOBACTERIA; HALOFERAX VOLCANII; NATRIALBA MAGADII;

EID: 50649105260     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-008-0174-6     Document Type: Article

References (36)

1. A Bolhuis 2002 Protein transport in the halophilic archaeon Halobacterium sp. NRC-1: a major role for the twin-arginine translocation pathway? Microbiology 148 3335 3346
2. Cline SW, Pfeifer F, Doolittle WF (1995) Transformation of halophilic archaea. In: DasSarma S, Fleischmann EM (eds) Archaea, a laboratory manual halophiles, Cold Spring Harbor Laboratory Press, New York, pp 197-204
3. RE De Castro JA Maupin-Furlow MI Giménez MK Herrera Seitz JJ Sánchez 2006 Haloarchaeal proteases and proteolytic systems FEMS Microbiol Rev 30 17 35
4. S Diaz F Pérez-Pomares C Pire J Ferrer MJ Bonete 2006 Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei Extremophiles 10 105 115
5. Dyall-Smith M (2006) The halohandbook: protocols for haloarchaeal genetics. http://www.microbiol.unimelb.edu.au/people/dyallsmith/resources/ halohandbook/index.html. p 140
6. P Edman G Begg 1967 A protein sequenator Eur J Biochem 1 80 91
7. M Falb F Pfeifer P Palm K Rodewald V Hickmann J Tittor D Oesterhelt 2005 Living with two extremes: conclusions from the genome sequences of Natronomonas pharaonis Gent Res 15 336 1343
8. J Feng HC Liu JF Chu PJ Zhou JA Tang SJ Liu 2006 Genetic cloning and functional expression in Escherichia coli of an archaeorhodopsin gene from Halorubrum xinjiangense Extremophiles 10 29 33
9. G Georgiu L Segatori 2005 Preparative expression of secreted proteins in bacteria: status report and future prospects Curr Opin Biotechnol 16 538 545
10. MI Giménez CA Studdert JJ Sánchez RE De Castro 2000 Extracellular protease of Natrialba magadii: purification and biochemical characterization Extremophiles 4 181 188
11. MI Giménez K Dilks M Pohlschröder 2007 Haloferax volcanii twin-arginine translocation substates include secreted soluble, C-terminally anchored and lipoproteins Mol Microbiol 66 1597 1606
12. GW Hutcheon N Vasisht A Bolhuis 2005 Characterization of a highly stable α-amylase from the halophilic archaeon Haloarcula hispanica Extremophiles 9 487 495
13. S Hou SW Larsen D Boudko CW Riley E Karatan E Zimmer W Ordal M Alam 2000 Myoglobin-like aerotaxis transducers in archaea and bacteria Nature 403 540 544
14. Y Kannan Y Koga Y Inou M Hakuri M Takagi T Imanaka M Morikawa S Kanaya 2001 Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence Appl Environm Microbiol 67 2445 2452
15. M Kamekura Y Seno ML Holmes ML Dyall-Smith 1992 Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172 P1) and expression of the gene in Haloferax volcanii J Bacteriol 174 736 742
16. M Kamekura Y Seno ML Dyall-Smith 1996 Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei; gene cloning, expression and structural studies Biochim Biophys Acta 1294 159 167
17. SJ Kaczowka JA Maupin-Furlow 2003 Subunit topology of two 20S proteasomes from Haloferax volcanii J Bacteriol 185 165 174
18. MG Marinus NR Morris 1973 Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12 J Bacteriol 114 1143 1150
19. M Mevarech F Frolow LM Gloss 2000 Halophilic enzymes: proteins with a grain of salt Biophys Chem 86 155 64
20. Ng WV, Yang CF, Halladay JT, Arora P, DasSarma S (1995) Isolation of genomic and plasmid DNAs from Halobacterium halobium. In: DasSarma S, Fleischmann EM (eds) Archaea, a laboratory manual. Halophiles, Cold Spring Harbor Laboratory Press, New York, pp 179-184
21. JR Palmer CJ Daniels 1995 In vivo definition of an archaeal promoter J Bacteriol 177 1844 1849
22. Place AR (1995) Codon usage tables for halophilic archaea. In: DasSarma S, Fleischmann EM (eds) Archaea, a laboratory manual. Halophiles, Cold Spring Harbor Laboratory Press, New York, pp 259-261
23. MB Rao AM Tanksale MS Ghatge VV Desphande 1998 Molecular and biotechnological aspects of microbial proteases Microbiol Mol Biol Rev 62 597 635
24. JN Reeve 2003 Archaeal chromatin and transcription Mol Microbiol 48 587 598
25. F Rodríguez-Valera G Juez DJ Kushner 1983 Halobacterium mediterranei sp. nov J Syst Appl Microbiol 4 369 381
26. RW Rose T Bruser JC Kissinger M Pohlschröder 2002 Adaptation of protein secretion to extremely high-salt conditions by extensive use of the twin-arginine translocation pathway Mol Microbiol 45 943 950
27. DM Ruiz RE De Castro 2007 Effect of organic solvents on the activity and stability of an extracellular protease secreted by the haloalkaliphilic archaeon Natrialba magadii J Ind Microbiol Biotechnol 34 111 115
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. CSH Press, New York
29. F Sanger AR Nicklen Coulson 1977 DNA sequencing with chain-terminating inhibitors Proc Natl Acad Sci USA 74 5463 5467
30. W Shi XF Tang Y Huang F Gan B Tang P Shen 2006 An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization Extremophiles 10 599 606
31. H Stan-Lotter E Doppler M Jarosh C Radax C Gruber KI Inatomi 1999 Isolation of a chymotrypsinogen B-like enzyme from the archaeon Natronomonas pharaonis and other halobacteria Extremophiles 3 153 161
32. CA Studdert MK Herrera Seitz MI Plasencia Gil JJ Sánchez RE De Castro 2001 Purification and biochemical characterization of the haloalkaliphilic archaeon Natronococcus occultus extracellular serine protease J Basic Microbiol 41 375 383
33. BJ Tindall AA Mills WD Grant 1984 Natronobacterium gen nov. and Natronococcus gen. nov. Two genera of haloalkaliphilic archaebacteria Syst Appl Microbiol 5 41 57
34. M Vidyasagar S Prakash C Litchfield K Sreeramulu 2006 Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101 Archaea 2 51 57
35. D Wendoloski C Ferrer ML Dyall-Smith 2001 A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2 Microbiology 147 949 954
36. Yu TX (1991) Proteases of haloalkaliphiles. In: Horikoshi K, Grant WD (eds) Superbugs: microorganisms in extreme environments, Springer, NewYork, pp 76-83