Real time PCR detection of Piscirickettsia salmonis from formalin-fixed paraffin-embedded tissues

Journal of Fish Diseases

Volumn 31, Issue 10, 2008, Pages 747-753

  Karatas, S ^b,c   Mikalsen, J ^b   Steinum, T M ^b   Taksdal, T ^b   Bordevik, M ^a   Colquhoun, D J ^a,b  

^a NORWEGIAN VETERINARY INSTITUTE   (Norway)

^b ISTANBUL UNIVERSITY   (Turkey)

^c ZOETIS   (United States)

Author keywords

Detection; Embedded tissues; Piscirickettsia salmonis; Polymerase chain reaction

Indexed keywords

FIXATIVE; FORMALDEHYDE; PARAFFIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; FISH DISEASE; ISOLATION AND PURIFICATION; PISCIRICKETTSIA; PISCIRICKETTSIOSIS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TIME; TISSUE FIXATION;

ANIMALS; FISH DISEASES; FIXATIVES; FORMALDEHYDE; PARAFFIN EMBEDDING; PISCIRICKETTSIA; PISCIRICKETTSIACEAE INFECTIONS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TISSUE FIXATION;

PISCIRICKETTSIA SALMONIS; SALMONIDAE;

EID: 50649105044     PISSN: 01407775     EISSN: 13652761     Source Type: Journal    
DOI: 10.1111/j.1365-2761.2008.00948.x     Document Type: Article

References (31)

1. Aguayo J., Miquel A., Aranki N., Jamett A., Valenzuela P.D. Burzio L.O. (2002) Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies. Diseases of Aquatic Organisms 49, 33 38.
2. Altman D.G. (1991) Practical Statistics for Medical Research. Chapman & Hall, London.
3. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W. Lipman D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389 3402.
4. Birkbeck T.H., Griffen A.A., Reid H.I., Laidler L.A. Wadsworth S. (2004) Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells. Infection and Immunity 72, 3693 3694.
5. Bravo S. Campos M. (1989) Coho salmon syndrome in Chile. American Fisheries Society Newsletter 17, 3.
6. Brocklebank J.R., Evelyn T.P., Speare D.J. Armstrong R.D. (1993) Rickettsial septicemia in farmed Atlantic and chinook salmon in British Columbia: clinical presentation and experimental transmission. Canadian Veterinary Journal 34, 745 748.
7. Brunborg I.M., Moldal T. Jonassen C.M. (2004) Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. Journal of Virological Methods 122, 171 178.
8. Chan P.K., Chan D.P., To K.F., Yu M.Y., Cheung J.L. Cheng A.F. (2001) Evaluation of extraction methods from paraffin wax embedded tissues for PCR amplification of human and viral DNA. Journal of Clinical Pathology 54, 401 403.
9. Coombs N.J., Gough A.C. Primrose J.N. (1999) Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic Acids Research 27, e12.
10. Corbeil S., McColl K.A. Crane M.S.J. (2003) Development of a TaqMan quantitative PCR assay for the identification of Piscirickettsia salmonis. Bulletin of the Euopean Association of Fish Pathologists 23, 95 101.
11. Corbeil S., Hyatt A.D. Crane M.S.J. (2005) Characterisation of an emerging rickettsia-like organism in Tasmanian farmed Atlantic salmon Salmo salar. Diseases of Aquatic Organisms 64, 37 44.
12. Cvitanich J.D., Garate O.N. Smith C.E. (1991) The isolation of a rickettsia-like organism causing disease and mortality in Chilean salmonids and its confirmation by Koch's postulates. Journal of Fish Diseases 14, 121 145.
13. Flesjå K.I., Hjeltnes B., Johansen R., Norheim K., Olsen A.B. Skjelstad H.R. (2004) The Health Situation in Salmonid Fish. (In Norwegian). Havbruksrapport 2004 (Annual Aquaculture Report 2004 Institute of Marine Research, Bergen, Norway.
14. Fryer J.L., Lannan C.N., Garces L.H., Larenas J.J. Smith P.A. (1990) Isolation of a rickettsiales-like organism from diseased coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathology 25, 107 114.
15. Fryer J.L., Lannan C.N., Giovannoni S.J. Wood N.D. (1992) Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. International Journal of Systemic Bacteriology 42, 120 126.
16. Kampen A.H., Tollersrud T., Larsen S., Roth J.A., Frank D.E. Lund A. (2004) Repeatability of flow cytometric and classical measurement of phagocytosis and respiratory burst in bovine polymorphonuclear leukocytes. Veterinary Immunology and Immunopathology 97, 105 114.
17. Lannan C.N. Fryer J.L. (1991) Recommended methods for inspection of fish for the salmonid rickettsia. Bulletin of the European Association of Fish Pathologists 11, 135 136.
18. Lannan C.N., Ewing S.A. Fryer J.L. (1991) A fluorescent antibody test for detection of the rickettsia causing disease in Chilean salmonids. Journal of Aquatic Animal Health 3, 229 234.
19. Marshall S., Heath S., Henriquez V. Orrego C. (1998) Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR. Applied and Environmental Microbiology 64, 3066 3069.
20. Mauel M.J., Giovannoni S.J. Fryer J.L. (1996) Development of polymerase chain reaction assays for detection, identification, and differentiation of Piscirickettsia salmonis. Diseases of Aquatic Organisms 26, 189 195.
21. Mauel M.J., Giovannoni S.J. Fryer J.L. (1999) Phylogenetic analysis of Piscirickettsia salmonis by 16S, internal transcribed spacer (ITS) and 23S ribosomal DNA sequencing. Diseases of Aquatic Organisms 35, 115 123.
22. McCarthy U., Steiropoulos N.A., Thompson K.D., Adams A., Ellis A.E. Ferguson H.W. (2005) Confirmation of Piscirickettsia salmonis as a pathogen in European sea bass Dicentrarchus labrax and phylogenetic comparison with salmonid strains. Diseases of Aquatic Organisms 64, 107 119.
23. Mikalsen J., Olsen A.B., Tengs T. Colquhoun D.J. (2007a) Francisella philomiragia subsp. noatunensis subsp. nov., isolated from farmed Atlantic cod (Gadus morhua L.). International Journal of Systematic and Evolutionary Microbiology 57, 1960 1965.
24. Mikalsen J., Skjaervik O., Wiik-Nielsen J., Wasmuth M.A. Colquhoun D.J. (2007b) Agar culture of Piscirickettsia salmonis, a serious pathogen of farmed salmonid and marine fish. FEMS Microbiology Letters 278, 43 47.
25. Nitsche A., Steuer N., Schmidt C.A., Landt O. Siegert W. (1999) Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA. Clinical Chemistry 45, 1932 1937.
26. Olsen A.B., Melby H.P., Speilberg L., Evensen O. Hastein T. (1997) Piscirickettsia salmonis infection in Atlantic salmon Salmo salar in Norway - epidemiological, pathological and microbiological findings. Diseases of Aquatic Organisms 31, 35 48.
27. Olsen A.B., Mikalsen J., Rode M., Alfjorden A., Hoel E., Straum-Lie K., Haldorsen R. Colquhoun D.J. (2006) A novel systemic granulomatous inflammatory disease in farmed Atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella. Journal of Fish Diseases 29, 307 311.
28. Palmer R., Ruttledge M., Callanan K. Drinan E. (1996) A piscirickettsiosis-like disease in farmed Atlantic salmon in Ireland - isolation of the agent. Bulletin of the European Association of Fish Pathologists 17, 68 72.
29. Rodger H.D. Drinan E.M. (1993) Observation of a rickettsia-like organism in Atlantic salmon, Salmo salar L., in Ireland. Journal of Fish Diseases 16, 361 369.
30. Rojas M.V., Olivares P.J., Del Rio R. Marshall S.H. (2007) Characterization of a novel and genetically different small infective variant of Piscirickettsia salmonis. Microbial Pathogenesis 44 (5 370 378.
31. Venegas C.A., Contreras J.R., Larenas J.J. Smith P.A. (2004) DNA hybridization assays for the detection of Piscirickettsia salmonis in salmonid fish. Journal of Fish Diseases 27, 431 433.