Transformation of the Gram-positive honey bee pathogen, Paenibacillus larvae, by electroporation

Journal of Microbiological Methods

Volumn 75, Issue 2, 2008, Pages 325-328

  Murray, K Daniel ^a   Aronstein, Katherine A ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

American Foulbrood; Apis mellifera; Electroporation; Electrotransformation; Honey bee; Paenibacillus larvae

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CELL DENSITY; CULTURE MEDIUM; ELECTRIC CURRENT; ELECTROPORATION; ELECTROTRANSFORMATION; HONEYBEE; NONHUMAN; PAENIBACILLUS; PAENIBACILLUS LARVAE; PARAMETER; PLASMID; PRIORITY JOURNAL;

ANIMALS; BEES; CULTURE MEDIA; ELECTROPORATION; GRAM-POSITIVE ENDOSPORE-FORMING RODS; LARVA; PLASMIDS; SPORES, BACTERIAL; TRANSFORMATION, BACTERIAL;

APIS MELLIFERA; PAENIBACILLUS; PAENIBACILLUS LARVAE; POSIBACTERIA;

EID: 50549102142     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.07.007     Document Type: Article

References (24)

1. Alippi A.M., Lopez A.C., Reynaldi F.J., Grasso D.H., and Aguilar O.M. Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. Vet. Microbiol. 125 (2007) 290-303
2. Ash C., Priest F.G., and Collins M.D. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64 (1993) 253-260
3. Bakhiet N., and Stahly D.P. Studies on transfection and transformation of protoplasts of Bacillus larvae, Bacillus subtilis, and Bacillus popilliae. Appl. Environ. Microbiol. 49 (1985) 577-581
4. Benada O., Drobníková V., Kalachová L., and Ludvík J. Plasmid DNA in Bacillus larvae. J. Apicult. Res. 27 (1988) 35-39
5. Bio-Rad Laboratories, 1991. Electroprotocols Species List, Bacterial-Rev. A. Bio-Rad Laboratories, Hercules, CA. http://www.biorad.com/cmc_upload/Literature/210246/Bulletin_D035552.pdf.
6. Bodorova-Urgosikova J., Benada O., and Tichy P. Large scale isolation and partial characterization of plasmid DNA from B. larvae. Folia Microbiologica 37 (1992) 82-86
7. Committee on the Status of Pollinators in North America, National Research Council (2007), Status of Pollinators in North America. The National Academies Press, Washington D.C.
8. Cox-Foster D.L., Conlan S., Holmes E.C., Palacios G., Evans J.D., Moran N.A., Quan P.-L., Briese T., Hornig M., Geiser D.M., Martinson V., van Engelsdorp D., Kalkstein A.L., Drysdale A., Hui J., Zhai J., Cui L., Hutchison S.K., Simons J.F., Egholm M., Pettis J.S., and Lipkin W.I. A metagenomic survey of microbes in honey bee Colony Collapse Disorder. Science 318 (2007) 283-287
9. De Jong D. Varroa and other parasites of brood. Morse, R., Flottum, K. (1997), Honey bee pest, predators, and diseases, 3rd ed. A.I. Root Co., Medina, Ohio 280-327
10. Dingman D.W., and Stahly D.P. Medium promoting sporulation of Bacillus larvae and metabolism of medium components. Appl. Environ. Microbiol. 46 (1983) 860-869
11. Drobníková V., Richter V., Häusler J., and Pytelová I. Characterization of Bacillus larvae and related bacilli by chromatography of cell fatty acids. J. Apicult. Res. 33 (1994) 69-74
12. Honeybee Genome Sequencing Consortium. Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443 (2006) 931-949
13. Klee J., Besana A.M., Genersch E., Gisder S., Nanetti A., Tam D.Q., Chinh T.X., Puerta F., Ruz J.M., Kryger P., Message D., Hatjina F., Korpela S., Fries I., and Paxton R.J. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. J. Invert Pathol 96 (2007) 1-10
14. Miyagi T., Peng C.Y.S., Chuang R.Y., Mussen E.C., Spivak M.S., and Doi R.H. Verification of oxytetracycline-resistant American foulbrood pathogen Paenibacillus larvae in the United States. J. Invertebr. Pathol. 75 (2000) 95-96
15. Murray K.D., and Aronstein K.A. Oxytetracycline-resistance in the honey bee pathogen Paenibacillus larvae is encoded on novel plasmid pMA67. J. Apicult. Res. 45 (2006) 207-214
16. Murray K.D., Aronstein K.A., and de Leon J.H. Analysis of pMA67, a predicted rolling-circle replicating, mobilizable, tetracycline-resistance plasmid from the honey bee pathogen, Paenibacillus larvae. Plasmid 58 (2007) 89-100
17. Mussen E.C. Antibiotic-resistant American foulbrood. Am. Bee J. 140 (2000) 300-301
18. Neuendorf S., Hedtke K., Tangen G., and Genersch E. Biochemical characterization of different genotypes of Paenibacillus larvae subsp. larvae, a honey bee bacterial pathogen. Microbiology 150 (2004) 2381-2390
19. Pettis J.S., and Shimanuki H. Observations on the small hive beetle, Aethina tumida Murray, in the United States. Am. Bee J. 140 (2000) 152-155
20. Qin X., Evans J.D., Aronstein K.A., Murray K.D., and Weinstock G.M. Genome sequences of the honey bee pathogens Paenibacillus larvae and Ascosphaera apis. Insect Mol. Biol. 15 (2006) 715-718
21. Rosado A., Duarte G.F., and Seldin L. Optimization of electroporation procedure to transform B. polymyxa SCE2 and other nitrogen-fixing Bacillus. J. Microbiol. Methods 19 (1994) 1-11
22. Taylor L.D., and Burke W.F. Transformation of an entomopathic strain of Bacillus sphaericus by high voltage electroporation. FEMS Microbiol. Lett. 66 (1990) 125-128
23. Tryfona T., and Bustard M.T. Enhancement of biomolecule transport by electroporation: a review of theory and practical application to transformation of Corynebacterium glutamicum. Biotechnol. Bioeng. 93 (2006) 413-423
24. Zeigler, D.R., 1999. Electroporation of B. thuringiensis and B. cereus. In: Bacillus Genetic Stock Center Catalog of Strains, Seventh Edition, Part 2: Bacillus thuringiensis and Bacillus cereus. pp. 28-29. http://www.bgsc.org/Catalogs/Catpart2.pdf.