Non-invasive methods for determination of cellular growth in Podophyllum hexandrum suspension cultures

Biotechnology and Bioprocess Engineering

Volumn 7, Issue 6, 2002, Pages 331-334

  Chattopadhyay, Saurabh ^a   Bisaria, V S ^a   Scheper, T ^b   Srivastava, A K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b Leibniz Universität Hannover   (Germany)

Author keywords

Conductivity measurement; NADH measurement; On line biomass measurement; Podophyllum hexandrum

Indexed keywords

EID: 50449101185     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02933516     Document Type: Article

References (19)

1. Panda A. K., S. Mishra, V. S. Bisaria, and S. S. Bhojwani (1989) Plant cell reactors: A perspective. Enzyme Microb. Technol. 11:386-397.
2. Giri A. and M. L. Narasu (2000) Transgenic hairy roots: Recent trends and applications. Biotechnol. Adv. 18: 1-22.
3. Zhong J. J., K. Fujiyama, T. Seki, and T. Yoshida (1993) On-line monitoring of cell concentration of Perilla frutescens in a bioreator. Biotechnol. Bioeng. 42: 542-546.
4. Blom T. J. M., W. Kreis, F. V. Iren, and K. R. Libbenga (1992) A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures. Plant Cell. Rep. 11: 146-149.
5. Albiol J., C. Campmajo, C. Casas, and M. Poch (1995) Biomass estimation in plant cell cultures: A neural network approach. Biotechnol. Prog. 11: 88-92.
6. Ryu D. D. Y., S. O. Lee, and R. J. Romani (1990) Determination of growth rate for plant cell cultures: Comparative studies. Biotechnol. Bioeng. 35: 305-311.
7. Suresh B., T. Rajasekaran, S. R. Rao, K. S. M. S. Raghavarao, and G. A. Ravishankar (2001) Studies on osmolarity conductivity and mass transfer for selection of a bioreactor for Tagetes patula L. hairy roots. Process Biochem. 36: 987-993.
8. Duysens L. N. M. and J. Amesz (1957) Fluorescence spectrometry of reduced phosphopyridine nucleotide in intact cells in the near ultraviolet and visible region. Biochem. Biophys. Acta 24: 19-26.
9. Harrison D. E. F. and B. Chance (1970) Fluorometric technique for monitoring changes in the level of reduced nicotinamide nucleotides in continuous cultures of microorganisms. Appl. Microb. 19: 446-450.
10. Scheper T., A. Gebauer, and K. Schugerl (1987) Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli. Chem. Eng. J. 34: B7-B12.
11. Srivastava A. K. and B. Volesky (1991a) On-line fluorescence measurements in assessing culture metabolic activities. Appl. Microbiol. Biotechnol. 34: 450-457.
12. Srivastava A. K. and B. Volesky (1991b) NADH fluorescence in a carbon limited fermentation. Biotechnol. Bioeng. 38: 191-195.
13. Sriram G. and G. K. Sureshkumar (2001) Culture fluorescence dynamics in Xanthomonas campestris. Process Biochem. 36: 1167-1173.
14. Asali E. C., R. Muthurasan, and A. E. Humphrey (1992) Use of NAD(P)H-fluorescence for monitoring the response of starved cells of Catharanthus roseus in suspension to metabolic perturbations. J. Biotechnol. 23: 83-94.
15. Chattopadhyay S., A. K. Srivastava, S. S. Bhojwani, and V. S. Bisaria (2001) Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin. Biotechnol. Lett. 23: 2063-2066.
16. Petersen M. and A. W. Alfermann (2001) The production of cytotoxic lignans by plant cultures. Appl. Microbiol. Biotechnol. 55: 135-142.
17. Murashige T. and F. Skoog (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
18. Chattopadhyay S., A. K. Srivastava, and V. S. Bisaria (2002) Optimization of culture parameters for production of podophyllotoxin in suspension culture of Podophyllum hexandrum. Appl. Biochem. Biotechnol. (in press).
19. Taya M., M. Hegglin, J. E. Prenosil, and J. R. Bourne (1989) Online monitoring of cell growth in plant tissue cultures by conductometry Enzyme Microb. Technol. 11: 170-176.