Estimation of serum M-protein concentration from polyclonal immunoglobulins: An alternative to serum protein electrophoresis and standard immunochemical procedures

Clinical Chemistry and Laboratory Medicine

Volumn 46, Issue 8, 2008, Pages 1156-1162

  Bergón, Enrique ^a   Miravalles, Elena ^a  

^a HOSPITAL UNIVERSITARIO DE GETAFE   (Spain)

Author keywords

Fixed time nephelometry; Serum monoclonal protein quantification; Serum protein electrophoresis

Indexed keywords

IMMUNOGLOBULIN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOGLOBULIN M; M PROTEIN;

ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CHRONIC LYMPHATIC LEUKEMIA; DEVICE; HUMAN; IMMUNOCHEMISTRY; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MEASUREMENT; MONOCLONAL IMMUNOGLOBULINEMIA; NEPHELOMETRY; NONHODGKIN LYMPHOMA; PLASMACYTOMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ELECTROPHORESIS; SERUM; WALDENSTROEM MACROGLOBULINEMIA;

ANTIBODIES, MONOCLONAL; BLOOD CHEMICAL ANALYSIS; BLOOD PROTEIN ELECTROPHORESIS; HUMANS; IMMUNOCHEMISTRY; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN ISOTYPES; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULINS; NEPHELOMETRY AND TURBIDIMETRY;

EID: 50249126013     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2008.232     Document Type: Article

References (19)

1. Durie BG, Harousseau J-L, Miguel JS, Bladé J, Barlogie B, Anderson K, et al. International uniform response criteria for multiple myeloma. Leukemia 2006;20:1467-73.
2. Keren DF, Alexanian R, Goeken JA, Gorevic PD, Kyle RA, Tomar RH. Guidelines for clinical and laboratory evaluation of patients with monoclonal gammopathies. Arch Pathol Lab Med 1999;123:106-7.
3. Bergó n Jiménez E, Bergón Sendín M. Transferibilidad de resultados entre procedimientos electroforéticos para la valoración de un componente monoclonal. Quim Clin 1998;17:227-31.
4. Bergón E, Miranda I, Miravalles E. Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys®. Clin Chem Lab Med 2005;43:721-3.
5. Mussap M, Pietrogrande F, Ponchia S, Stefani PM, Sartori R, Plebani M. Measurement of serum monoclonal component: comparison between densitometry and capillary zone electrophoresis. Clin Chem Lab Med 2006;44:609-11.
6. Wuyts B, Bossuyt X, Verhoef G, Blanckaert N, Delanghe JR. Conflicting results between electrophoresis methods of serum M-proteins. Electrophoresis 2004;25:1548-50.
7. Riches PG, Sheldon J, Smith AM, Hobbs JR. Overestimation of monoclonal immunoglobulin by immunochemical methods. Ann Clin Biochem 1991;28:253-9.
8. Bossuyt X, Vranken G, Mariën G, Blanckaert N. Monoclonal IgM: difficulties with correct measurement. Ann Clin Biochem 2001;38:708-10.
9. Fifield R, Keller I. An immunochemical evaluation for the identification and typing of monoclonal proteins. Ann Clin Biochem 1990;27:327-34.
10. Chui SH, Lam WK, Lai KN. Light-chain ratios of immunoglobulins G, A, and M determined by enzyme immunoassay. Clin Chem 1990;36:501-2.
11. Bergón E, Miravalles E. Retrospective study of monoclonal gammopathies detected in the clinical laboratory of a Spanish healthcare district: 14-year series. Clin Chem Lab Med 2007;45:190-6.
12. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986307-10.
13. Dewitte K, Fierens C, Stöckl D, Thienpont LM. Application of the Bland-Altman plot for interpretation of methods-comparison studies: a critical investigation of its practice [letter]. Clin Chem 2002;48:799-801.
14. Altman DG, Bland JM. Commentary on quantifying agreement between two methods of measurement [letter]. Clin Chem 2002;48:801-2.
15. Luraschi P, Infusino I, Merlotti C, Franzini C. Analytical variation in the measurement of serum monoclonal component by capillary electrophoresis. Clin Chim Acta 2004;349:151-6.
16. Ford RP, Mitchell PEG, Fraser CG. Desirable performance characteristics and clinical utility of immunoglobulin and light-chain assays derived from data on biological variation. Clin Chem 1988;34:1733-6.
17. Petersen PH, Fraser CG, Westgard JO, Larsen ML. Analytical goal-setting for monitoring patients when two analytical methods are used. Clin Chem 1992;38:2256-60.
18. Bladé J, Samson D, Reece D, Apperley J, Björkstrand B, Gahrton G, et al. Criteria for evaluating disease response and progression in patients with multiple myeloma treated by high-dose therapy and haemopoietic stem cell transplantation. Br J Haematol 1998;102:1115-23.
19. Bergón Jimé nez E, Bergón Sendín E. Estimaciones de los factores de equivalencia entre las cadenas ligeras e isotipos de las inmunoglobulinas [abstract]. Quim Clin 1998;17:135.