Spore stage expression of a vegetative insecticidal gene increase toxicity of Bacillus thuringiensis subsp. aizawai SP41 against Spodoptera exigua

Journal of Biotechnology

Volumn 136, Issue 3-4, 2008, Pages 122-128

  Thamthiankul Chankhamhaengdecha, S ^a   Tantichodok, A ^b   Panbangred, Watanalai ^a,c  

^a Mahidol University   (Thailand)

^b Department of Agriculture   (Thailand)

^c MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Bacillus thuringiensis; RT PCR; Vegetative insecticidal protein

Indexed keywords

BACTERIOLOGY; BIOASSAY; CONCENTRATION (PROCESS); DIFFUSERS (OPTICAL); TOXICITY; TRANSCRIPTION;

BACILLUS THURINGIENSIS; CRY11AA; CULTURE SUPERNATANT; EXPRESSION LEVELS; INSECTICIDAL PROTEINS; LETHAL CONCENTRATIONS; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; RT-PCR; SDS-PAGE ANALYSIS; SPODOPTERA EXIGUA; SURFACE CONTAMINATIONS; TIME POINTS; VEGETATIVE INSECTICIDAL PROTEIN; WILD-TYPE;

STAGES;

ARMYWORM; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOASSAY; CONTROLLED STUDY; GENE EXPRESSION; LC 50; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPOROGENESIS; SUPERNATANT; VEGETATIVE INSECTICIDAL PROTEIN GENE; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL ASSAY; GENE EXPRESSION REGULATION, BACTERIAL; INHIBITORY CONCENTRATION 50; INSECTICIDES; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPODOPTERA; SPORES, BACTERIAL; TOXICITY TESTS;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR AIZAWAI; SPODOPTERA EXIGUA;

EID: 50149111306     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.05.013     Document Type: Article

References (29)

1. Arora N., Selvapandiyan A., Agrawal N., and Bhatnagar R.K. Relocating expression of vegetative insecticidal protein into mother cell of Bacillus thuringiensis. Biochem. Biophys. Res. Commun. 310 1 (2003) 158-162
2. Bernhard K., Schrempf H., and Goebel W. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J. Bacteriol. 133 2 (1978) 897-903
3. Chalfant R.B. A simplified technique for rearing the lesser corn stalk borer (Lepidoptera: Phyciditae). J. Ga. Entomol. Soc. 10 (1975) 32-33
4. Cosby W.M., and Zuber P. Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH. J. Bacteriol. 179 21 (1997) 6778-6787
5. Donovan W.P., Donovan J.C., and Engleman J.T. Gene knockout demonstrates that vip3A contributes to the pathogenesis of Bacillus thuringiensis toward Agrotis ipsilon and Spodoptera exigua. J. Invertebr. Pathol. 78 1 (2001) 45-51
6. Estruch J.J., Warren G.W., Mullins M.A., Nye G.J., Craig J.A., and Koziel M.G. Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Proc. Natl. Acad. Sci. U.S.A. 93 11 (1996) 5389-5394
7. Ferre J., and Van Rie J. Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Annu. Rev. Entomol. 47 (2002) 501-533
8. Finney D. Probit Analysis (1952), Cambridge University, Cambridge pp. 20-49
9. Gaidenko T.A., and Price C.W. General stress transcription factor sigmaB and sporulation transcription factor sigmaH each contribute to survival of Bacillus subtilis under extreme growth conditions. J. Bacteriol. 180 14 (1998) 3730-3733
10. Gould F., Martinez-Ramirez A., Anderson A., Ferre J., Silva F.J., and Moar W.J. Broad-spectrum resistance to Bacillus thuringiensis toxins in Heliothis virescens. Proc. Natl. Acad. Sci. U.S.A. 89 17 (1992) 7986-7990
11. Guttmann D.M., and Ellar D.J. Phenotypic and genotypic comparisons of 23 strains from the Bacillus cereus complex for a selection of known and putative B. thuringiensis virulence factors. FEMS Microbiol. Lett. 188 1 (2000) 7-13
12. Jianwu C., Fan S., Lixia T., Mujin T., Yongxia S., Jianxiu Y., and Yi P. Expression of Bacillus thuringiensis full-length and N-terminally truncated vip184 gene in an acrystalliferous strain of subspecies kurstaki. World J. Microbiol. Biotechnol. 19 (2003) 883-889
13. MacIntosh S.C., Stone T.B., Sims S.R., Hunst P.L., Greenplate J.T., Marrone P.G., Perlak F.J., Fischhoff D.A., and Fuchs R.L. Specificity and efficacy of purified Bacillus thuringiensis proteins against agronomically important insects. J. Invertebr. Pathol. 56 2 (1990) 258-266
14. Matsuno Y., Ano T., and Shoda M. High-efficiency transformation of Bacillus subtilis NB, 22, an antifungal antibiotic iturin producer, by electroporation. J. Ferm. Bioeng. 73 (1992) 261-264
15. McGaughey W.H., and Whalon M.E. Managing insect resistance to Bacillus thuringiensis toxins. Science 285 (1992) 1451-1455
16. Mesrati L.A., Tounsi S., Kamoun F., and Jaoua S. Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus thuringiensis. FEMS Microbiol. Lett. 247 1 (2005) 101-104
17. Miura K. Preparation of bacterial DNA by phenol-pH 9 RNase method. Methods Enzymol. 68 (1967) 543-545
18. Moar W.J., Pusztai-Carey M., Van Faassen H., Bosch D., Frutos R., Rang C., Luo K., and Adang M.J. Development of Bacillus thuringiensis CryIC resistance by Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae). Appl. Environ. Microbiol. 61 6 (1995) 2086-2092
19. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29 9 (2001) e45
20. Prieto-Samsonov D.L., Vazquez-Padron R.I., Ayra-Pardo C., Gonzalez-Cabrera J., and de la Riva G.A. Bacillus thuringiensis: from biodiversity to biotechnology. J. Ind. Microbiol. Biotechnol. 19 3 (1997) 202-219
21. Rojiani M.V., Jakubowski H., and Goldman E. Relationship between protein synthesis and concentrations of charged and uncharged tRNATrp in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87 4 (1990) 1511-1515
22. Schnepf E., Crickmore N., Van Rie J., Lereclus D., Baum J., Feitelson J., Zeigler D.R., and Dean D.H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62 3 (1998) 775-806
23. Shao Z., and Yu Z. Enhanced expression of insecticidal crystal proteins in wild Bacillus thuringiensis strains by a heterogeneous protein P20. Curr. Microbiol. 48 5 (2004) 321-326
24. Sirichotpakorn N., Rongnoparut P., Choosang K., and Panbangred W. Coexpression of chitinase and the cry11Aa1 toxin genes in Bacillus thuringiensis serovar israelensis. J. Invertebr. Pathol. 78 3 (2001) 160-169
25. Southern E.M. Detection of specific sequence among DNA fragment separated by gel electrophoresis. J. Mol. Biol. 98 (1975) 513-517
26. Thamthiankul S., Moar W.J., Miller M.E., and Panbangred W. Improving the insecticidal activity of Bacillus thuringiensis subsp. aizawai against Spodoptera exigua by chromosomal expression of a chitinase gene. Appl. Microbiol. Biotechnol. 65 2 (2004) 183-192
27. Wu D., and Federici B.A. Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein. Appl. Microbiol. Biotechnol. 42 5 (1995) 697-702
28. Wu Z.L., Guo W.Y., Qiu J.Z., Huang T.P., Li X.B., and Guan X. Cloning and localization of vip3A gene of Bacillus thuringiensis. Biotechnol. Lett. 26 18 (2004) 1425-1428
29. Yu C.G., Mullins M.A., Warren G.W., Koziel M.G., and Estruch J.J. The Bacillus thuringiensis vegetative insecticidal protein Vip3A lyses midgut epithelium cells of susceptible insects. Appl. Environ. Microbiol. 63 2 (1997) 532-536