Tsr-GFP accumulates linearly with time at cell poles, and can be used to differentiate 'old' versus 'new' poles, in Escherichia coli

Molecular Microbiology

Volumn 69, Issue 6, 2008, Pages 1427-1438

  Ping, Liyan ^a,b   Weiner, Beth ^b   Kleckner, Nancy ^b  

^a MAX PLANCK INSTITUTE FOR CHEMICAL ECOLOGY   (Germany)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOTACTIC PEPTIDE; GREEN FLUORESCENT PROTEIN; PROTEIN TSR; UNCLASSIFIED DRUG;

ARTICLE; CELL AGING; CELL LINEAGE; CELL POLARITY; CHEMOTAXIS; DIAGNOSTIC VALUE; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; FUSION GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; RELIABILITY; TSR GFP FUSION GENE;

ARTIFICIAL GENE FUSION; BACTERIAL PROTEINS; ESCHERICHIA COLI; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; MODELS, BIOLOGICAL; MODELS, MOLECULAR; RECOMBINANT FUSION PROTEINS; TIME FACTORS;

ESCHERICHIA COLI;

EID: 50049134923     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2008.06372.x     Document Type: Article

References (29)

1. Ausmees, N., Jacobsson, K. Lindberg, M. (2001) A unipolarly located, cell-surface-associated agglutinin, RapA, belongs to a family of Rhizobium-adhering proteins (Rap) in Rhizobium leguminosarum bv. trifolii. Microbiology 147 : 549 559.
2. Banno, S., Shiomi, D., Homma, M. Kawagishi, I. (2004) Targeting of the chemotaxis methylesterase/deamidase CheB to the polar receptor-kinase cluster in an Escherichia coli cell. Mol Microbiol 53 : 1051 1063.
3. Bates, D. Kleckner, N. (2005) Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation. Cell 121 : 899 911.
4. Bates, D., Epstein, J., Boye, E., Fahrner, K., Berg, H. Kleckner, N. (2005) The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle. Mol Microbiol 57 : 380 391.
5. Brandon, L.D., Goehring, N., Janakiraman, A., Yan, A.W., Wu, T., Beckwith, J. Goldberg, M.B. (2003) IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed. Mol Microbiol 50 : 45 60.
6. Carlsson, F., Stalhammar-Carlemalm, M., Flardh, K., Sandin, C., Carlemalm, E. Lindahl, G. (2006) Signal sequence directs localized secretion of bacterial surface proteins. Nature 442 : 943 946.
7. Clarke, D.J. Maaloe, O. (1967) DNA replication and the division cycle of Escherichia coli B/r. J Mol Biol 23 : 99 112.
8. Dietzel, I., Kolb, V. Boos, W. (1978) Pole cap formation in Escherichia coli following induction of the maltose-binding protein. Arch Microbiol 118 : 207 218.
9. Jain, S., van Ulsen, P., Benz, I., Schmidt, M.A., Fernandez, R., Tommassen, J. Goldberg, M.B. (2006) Polar localization of the autotransporter family of large bacterial virulence proteins. J Bacteriol 188 : 4841 4850.
10. Kim, K.K., Yokota, H. Kim, S.H. (1999) Four-helical-bundle structure of the cytoplasmic domain of a serine chemotaxis receptor. Nature 400 : 787 792.
11. Kollmann, M., Lovdok, L., Bartholome, K., Timmer, J. Sourjik, V. (2005) Design principles of a bacterial signalling network. Nature 438 : 504 507.
12. Laus, M.C., Logman, T.J., Lamers, G.E., van Brussel, A.A., Carlson, R.W. Kijne, J.W. (2006) A novel polar surface polysaccharide from Rhizobium leguminosarum binds host plant lectin. Mol Microbiol 59 : 1704 1713.
13. Liberman, L., Berg, H.C. Sourjik, V. (2004) Effect of chemoreceptor modification on assembly and activity of the receptor-kinase complex in Escherichia coli. J Bacteriol 186 : 6643 6646.
14. Link, A.J., Phillips, D. Church, G.M. (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J Bacteriol 179 : 6228 6237.
15. Lybarger, S.R. Maddock, J.R. (2000) Differences in the polar clustering of the high- and low-abundance chemoreceptors of Escherichia coli. Proc Natl Acad Sci USA 97 : 8057 8062.
16. McNulty, C., Thompson, J., Barrett, B., Lord, L., Andersen, C. Roberts, I.S. (2006) The cell surface expression of group 2 capsular polysaccharides in Escherichia coli: the role of KpsD, RhsA and a multi-protein complex at the pole of the cell. Mol Microbiol 59 : 907 922.
17. Mileykovskaya, E. Dowhan, W. (2000) Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange. J Bacteriol 182 : 1172 1175.
18. de Pedro, M.A., Quintela, J.C., Holtje, J.V. Schwarz, H. (1997) Murein segregation in Escherichia coli. J Bacteriol 179 : 2823 2834.
19. Shapiro, L., McAdams, H.H. Losick, R. (2002) Generating and exploiting polarity in bacteria. Science 298 : 1942 1946.
20. Shiomi, D., Banno, S., Homma, M. Kawagishi, I. (2005) Stabilization of polar localization of a chemoreceptor via its covalent modifications and its communication with a different chemoreceptor. J Bacteriol 187 : 7647 7654.
21. Sourjik, V. Berg, H.C. (2004) Functional interactions between receptors in bacterial chemotaxis. Nature 428 : 437 441.
22. Stewart, E.J., Madden, R., Paul, G. Taddei, F. (2005) Aging and death in an organism that reproduces by morphologically symmetric division. PLoS Biol 3 : e45.
23. Takeuchi, S., DiLuzio, W.R., Weibel, D.B. Whitesides, G.M. (2005) Controlling the shape of filamentous cells of Escherichia coli. Nano Lett 5 : 1819 1823.
24. Thiem, S. Sourjik, V. (2008) Stochastic assembly of chemoreceptor clusters in Escherichia coli. Mol Microbiol 68 : 1228 1236.
25. Thiem, S., Kentner, D. Sourjik, V. (2007) Positioning of chemosensory clusters in E. coli and its relation to cell division. EMBO J 26 : 1615 1623.
26. Wang, X., Liu, X., Possoz, C. Sherratt, D.J. (2006) The two Escherichia coli chromosome arms locate to separate cell halves. Genes Dev 20 : 1727 1731.
27. Wetzel, B.K., Spicer, S.S., Dvorak, H.F. Heppel, L.A. (1970) Cytochemical localization of certain phosphatases in Escherichia coli. J Bacteriol 104 : 529 542.
28. Woldringh, C.L. (2005) Is Escherichia coli getting old? Bioessays 27 : 770 774.
29. Zhang, P., Khursigara, C.M., Hartnell, L.M. Subramaniam, S. (2007) Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy. Proc Natl Acad Sci USA 104 : 3777 3781.