Detection of reticulated platelets in whole blood of rats using flow cytometry

Experimental and Toxicologic Pathology

Volumn 60, Issue 6, 2008, Pages 443-448

  Pankraz, Alexander ^a   Ledieu, David ^b   Pralet, Dominique ^b   Provencher Bolliger, Anne ^b  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Bone marrow suppression; Immune mediated thrombocytopenia; Megakaryopoiesis; PSAIG; Reticulated platelets; Thrombopoiesis

Indexed keywords

CD61 ANTIGEN; EDETIC ACID; PARAFORMALDEHYDE; RNA;

ANEMIA; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; FLOW CYTOMETRY; LIGHT SCATTERING; NONHUMAN; RAT; ROOM TEMPERATURE; STAINING; THROMBOCYTE; THROMBOCYTOPENIA; THROMBOCYTOPOIESIS;

ANIMALS; BLOOD PLATELETS; FLOW CYTOMETRY; PLATELET COUNT; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS; TEMPERATURE; THROMBOPOIESIS; TIME FACTORS; TOXICITY TESTS;

ANIMALIA; RATTUS;

EID: 49949110498     PISSN: 09402993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.etp.2008.05.005     Document Type: Article

References (33)

1. Ault K.A. Flow cytometric measurement of platelet-associated immunoglobulin. Pathol Immunopathol Res 7 (1988) 395-408
2. Ault K.A., and Mitchell J. Analysis of platelets by flow cytometry. Methods Cell Biol 42 (1994) 275-294
3. Ault K.A., and Knowles C. In vivo biotinylation demonstrates that reticulated platelets are the youngest platelets in circulation. Exp Hematol 23 (1995) 996-1001
4. Ault K.A., Rinder H.M., Mitchell J., Carmody M.B., Vary C.P., and Hillman R.S. The significance of platelets with increased RNA content (reticulated platelets). A measure of the rate of thrombopoiesis. Am J Clin Pathol 98 (1992) 637-646
5. Briggs C., Kunka S., Hart D., Oguni S., and Machin S.J. Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia. Br J Haematol 126 (2004) 93-99
6. Briggs C., Harrison P., and Machin S.J. Continuing developments with the automated platelet count. Int J Lab Hematol 29 (2007) 77-91
7. Dale G.L., Friese P., Hynes L.A., and Burstein S.A. Demonstration that thiazole-orange-positive platelets in the dog are less than 24 h old. Blood 85 (1995) 1822-1825
8. Diquattro M, Gagliano F, Calabro GM, Tommasi M, Scott CS, Manusco G, et al., Relationships between platelet counts, platelet volumes and reticulated platelets in patients with ITP: evidence for significant platelet count inaccuracies with conventional instrument methods. Int J Lab Hematol, 2008; Epub ahead of print.
9. Hanahachi A., Kano R., Hasegawa A., Moritomo T., Watari T., and Tsujimoto H. Thiazole orange-positive platelets in healthy and thrombocytopenic dogs. Vet Rec 149 (2001) 122-123
10. Hanahachi A., Kano R., Hasegawa A., and Watari T. Thiazole orange-positive platelets in a dog with idiopathic thrombocytopenic purpura. Vet Rec 150 (2002) 48-49
11. Ingram M., and Coopersmith A. Reticulated platelets following acute blood loss. Br J Haematol 17 (1969) 225-229
12. Ivory K., Sarria B., Fairweather-Tait S.J., and Hughes D.A. Reticulated platelets interfere with flow cytometric reticulocyte counts. Int J Lab Hematol 29 (2007) 352-360
13. Kaito K., Otsubo H., Usui N., Yoshida M., Tanno J., Kurihara E., et al. Platelet size deviation width, platelet large cell ratio, and mean platelet volume have sufficient sensitivity and specificity in the diagnosis of immune thrombocytopenia. Br J Haematol 128 (2005) 698-702
14. Kickler T.S., Oguni S., and Borowitz M.J. A clinical evaluation of high fluorescent platelet fraction percentage in thrombocytopenia. Am J Clin Pathol 125 (2006) 282-287
15. Kienast J., and Schmitz G. Flow cytometric analysis of thiazole orange uptake by platelets: a diagnostic aid in the evaluation of thrombocytopenic disorders. Blood 75 (1990) 116-121
16. Kuo Y., Yang K.D., Yang M., Huang M.L., Lin C., Lin F., et al. Reactive thrombocytosis alone does not affect the patency of microvascular anastomosis in the splenectomy rat. Plast Reconstr Surg 110 (2002) 812-817
17. Kuter D.J. Thrombopoietin: biology and clinical applications. Oncologist 1 (1996) 98-106
18. Lazarus A.H., Wright J.F., Blanchette V., and Freedman J. Analysis of platelets by flow cytometry. Transfusion Sci 16 (1995) 353-361
19. Michimoto T., Okamura T., Suzuki K., Watari T., Kano R., and Hasegawa A. Thiazole orange positive platelets in a dog with Evans' syndrome. J Vet Med Sci 66 (2004) 1305-1306
20. Numbenjapon T., Mahapo N., Pornvipavee R., Sriswasdi C., Mongkonsritragoon W., Leelasiri A., et al. A prospective evaluation of normal mean platelet volume in discriminating hyperdestructive thrombocytopenia from hypoproductive thrombocytopenia. Int J Lab Hematol (2007) 1-7 [Online Early Article]
21. O'Malley C.J., Rasko J.E., Basser R.L., McGrath K.M., Cebon J., Grigg A.P., et al. Administration of pegylated recombinant human megakaryocyte growth and development factor to humans stimulates the production of functional platelets that show no evidence of in vivo activation. Blood 88 (1996) 3288-3298
22. Ogura M., Yasuno M., Kobayashi Y., and Tsukada T. Clinical significance of platelet volume indices estimated by automated blood cell analyzer. Rinsho Byori 43 (1995) 385-390
23. Okamura T., Hanahachi A., Kano R., Hasegawa A., Kurashige A., and Tsujimoto H. Thiazole orange-positive platelets in cats with thrombocytopenia induced by cyclophosphamide. Vet Rec 152 (2003) 506-507
24. Pankraz A., Bauer N., Moritz A., Provencher-Bolliger A., and Roman D. Comparative assessment of thrombopoiesis of rats and dogs with the ADVIA120 analyzer and by detection of reticulated platelets. Vet Pathol 43 (2006) 854
25. Rinder H.M., Munz U.J., Ault K.A., Bonan J.L., and Smith B.R. Reticulated platelets in the evaluation of thrombopoietic disorders. Arch Pathol Lab Med 117 (1993) 606-610
26. Robinson M.S., Harrison C., Mackie I.J., Machin S.J., and Harrison P. Reticulated platelets in primary and reactive thrombocytosis. Br J Haematol 101 (1998) 388-389
27. Schmitz G., Rothe G., Ruf A., Barlage S., Tschope D., Clemetson K.J., et al. European working group on clinical cell analysis: consensus protocol for the flow cytometric characterisation of platelet function. Thromb Haemost 79 (1998) 885-896
28. Smith R., and Thomas J.S. Quantitation of reticulated platelets in healthy dogs and in nonthrombocytopenic dogs with clinical disease. Vet Clin Pathol 31 (2002) 26-32
29. Solberg H.E., International Federation of Clinical Chemistry (IFCC), Scientific Committee, Clinical Section, Expert Panel on Theory of Reference Values, International Committee for Standardization in Haematology (ICSH), and Standing Committee on Reference Values. Approved recommendation (1986) on the theory of reference values: Part 1, The concept of reference values. J Clin Chem Clin Biochem 25 (1987) 337-342
30. Stockham S.L., and Scott M.A. Fundamentals of clinical pathology (2002), Iowa State Press 15
31. Thrall M.A., Baker D.C., Campbell T.W., DeNicola D., Fettmann M.J., Lassen E.D., et al. Veterinary hematology and clinical chemistry (2004), Lippincott Williams and Wilkins
32. Wang C., Smith B.R., Ault K.A., and Rinder H.M. Reticulated platelets predict platelet count recovery following chemotherapy. Transfusion 2 (2002) 368-374
33. Zucker-Franklin D., and Karpatkin S. Red-cell and platelet fragmentation in idiopathic autoimmune thrombocytopenic purpura. N Engl J Med 297 (1977) 517-533