Platelet lysate stimulates wound repair of HaCaT keratinocytes

British Journal of Dermatology

Volumn 159, Issue 3, 2008, Pages 537-545

  Ranzato, E ^a   Patrone, M ^a   Mazzucco, L ^b   Burlando, B ^a  

^a UNIVERSITY OF EASTERN PIEDMONT   (Italy)

^b C Arrigo Children's Hospital   (Italy)

Author keywords

Cell migration; ERK1 2; p38; Platelet lysate; Scratch wound assay

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 2 MORPHOLINO 8 PHENYLCHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; CALCIUM ION; CRYSTAL VIOLET; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE P38; WORTMANNIN;

ARTICLE; CELL LYSATE; CELL METABOLISM; CELL MIGRATION; CELL PROLIFERATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; PRIORITY JOURNAL; THROMBOCYTE; WESTERN BLOTTING; WOUND CLOSURE; WOUND HEALING;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANALYSIS OF VARIANCE; BLOOD PLATELETS; BLOTTING, WESTERN; CALCIUM; CALCIUM SIGNALING; CELL LINE; CELL MOVEMENT; COCULTURE TECHNIQUES; ENZYME ACTIVATION; HUMANS; KERATINOCYTES; MAP KINASE SIGNALING SYSTEM; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOGEN-ACTIVATED PROTEIN KINASE 3; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PLATELET COUNT; WOUND HEALING;

EID: 49749122706     PISSN: 00070963     EISSN: 13652133     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2008.08699.x     Document Type: Article

References (46)

1. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during wound healing. Exp Cell Res 2005 304 : 274 86.
2. Gillitzer R, Goebeler M. Chemokines in cutaneous wound healing. J Leukoc Biol 2001 69 : 513 21.
3. Harding KG, Morris HL, Patel GK. Science, medicine and the future: healing chronic wounds. BMJ 2002 324 : 160 3.
4. Shirakawa M, Isseroff RR. Topical negative pressure devices: use for enhancement of healing chronic wounds. Arch Dermatol 2005 141 : 1449 53.
5. Robson MC. The role of growth factors in the healing of chronic wounds. Wound Repair Regen 1997 5 : 7 12.
6. Mazzucco L, Medici D, Serra M et al. The use of autologous platelet gel to treat difficult-to-heal wounds: a pilot study. Transfusion 2004 44 : 1013 18.
7. Marx RE, Carlson ER, Eichstaedt RM et al. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998 85 : 638 46.
8. Gehring S, Hoerauf H, Laqua H et al. Preparation of autologous platelets for the ophthalmologic treatment of macular holes. Transfusion 1999 39 : 144 8.
9. Martin P. Wound healing - aiming for perfect skin regeneration. Science 1997 276 : 75 81.
10. Sanchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review Int J Oral Maxillofac Implants 2003 18 : 93 103.
11. Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002 30 : 97 102.
12. Crovetti G, Martinelli G, Issi M et al. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004 30 : 145 51.
13. Borzini P, Mazzucco L. Platelet gels and releasates. Curr Opin Hematol 2005 12 : 473 9.
14. Okuda K, Kawase T, Momose M et al. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol 2003 74 : 849 57.
15. Soffer E, Ouhayoun JP, Anagnostou F. Fibrin sealants and platelet preparations in bone and periodontal healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003 95 : 521 8.
16. Kilian O, Flesch I, Wenisch S et al. Effects of platelet growth factors on human mesenchymal stem cells and human endothelial cells in vitro. Eur J Med Res 2004 9 : 337 44.
17. Jacinto A, Martinez-Arias A, Martin P. Mechanisms of epithelial fusion and repair. Nat Cell Biol 2001 3 : E117 23.
18. Boukamp P, Petrussevska RT, Breitkreutz D et al. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988 106 : 761 71.
19. Schoop VM, Mirancea N, Fusenig NE. Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. J Invest Dermatol 1999 112 : 343 53.
20. Matsuura K, Kuratani T, Gondo T et al. Promotion of skin epithelial cell migration and wound healing by a 2-benzazepine derivative. Eur J Pharmacol 2007 563 : 83 7.
21. Charvat S, Le Griel C, Chignol MC et al. Ras-transfection up-regulated HaCaT cell migration: inhibition by Marimastat. Clin Exp Metastasis 1999 17 : 677 85.
22. Council of Europe. Guide to Preparation, Use and Quality Assurance of Blood Components, 13th edn. Croton-on-Hudson, NY : Council of Europe Publications, Manhattan Publishing Company, 2007.
23. Pignatelli P, Pulcinelli FM, Ciatti F et al. Acid citrate dextrose (ACD) formula A as a new anticoagulant in the measurement of in vitro platelet aggregation. J Clin Lab Anal 1995 9 : 138 40.
24. Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 1985 260 : 3440 50.
25. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970 227 : 680 5.
26. Eastment CT, Sirbasku DA. Human platelet lysate contains growth factor activities for established cell lines derived from various tissues of several species. In Vitro 1980 16 : 694 705.
27. Celotti F, Colciago A, Negri-Cesi P et al. Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts: role of platelet-derived growth factor and transforming growth factor-beta. Wound Repair Regen 2006 14 : 195 202.
28. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003 83 : 835 70.
29. Matsubayashi Y, Ebisuya M, Honjoh S et al. ERK activation propagates in epithelial cell sheets and regulates their migration during wound healing. Curr Biol 2004 14 : 731 5.
30. Clark RA, Lanigan JM, DellaPelle P et al. Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization. J Invest Dermatol 1982 79 : 264 9.
31. Castelnovo L, Dosquet C, Gaudric A et al. Human platelet suspension stimulates porcine retinal glial proliferation and migration in vitro. Invest Ophthalmol Vis Sci 2000 41 : 601 9.
32. Lansdown AB. Calcium: a potential central regulator in wound healing in the skin. Wound Repair Regen 2002 10 : 271 85.
33. Cheng HP, Wei S, Wei LP, Verkhratsky A. Calcium signaling in physiology and pathophysiology. Acta Pharmacol Sin 2006 27 : 767 72.
34. Leiper LJ, Walczysko P, Kucerova R et al. The roles of calcium signaling and ERK1/2 phosphorylation in a Pax6+/- mouse model of epithelial wound-healing delay. BMC Biol 2006 4 : 27.
35. Tournier JM, Maouche K, Coraux C et al. alpha3alpha5beta2-Nicotinic acetylcholine receptor contributes to the wound repair of the respiratory epithelium by modulating intracellular calcium in migrating cells. Am J Pathol 2006 168 : 55 68.
36. Klepeis VE, Cornell-Bell A, Trinkaus-Randall V. Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells. J Cell Sci 2001 114 : 4185 95.
37. Cole J, Tsou R, Wallace K et al. Early gene expression profile of human skin to injury using high-density cDNA microarrays. Wound Repair Regen 2001 9 : 360 70.
38. Sharma GD, He J, Bazan HE. p38 and ERK1/2 coordinate cellular migration and proliferation in epithelial wound healing: evidence of cross-talk activation between MAP kinase cascades. J Biol Chem 2003 278 : 21989 97.
39. Hedges JC, Dechert MA, Yamboliev IA et al. A role for p38(MAPK)/HSP27 pathway in smooth muscle cell migration. J Biol Chem 1999 274 : 24211 19.
40. Tangkijvanich P, Santiskulvong C, Melton AC et al. p38 MAP kinase mediates platelet-derived growth factor-stimulated migration of hepatic myofibroblasts. J Cell Physiol 2002 191 : 351 61.
41. Lai JP, Dalton JT, Knoell DL. Phosphatase and tensin homologue deleted on chromosome ten (PTEN) as a molecular target in lung epithelial wound repair. Br J Pharmacol 2007 152 : 1172 84.
42. Zhao M. PTEN: a promising pharmacological target to enhance epithelial wound healing. Br J Pharmacol 2007 152 : 1141 4.
43. Buffin-Meyer B, Crassous PA, Delage C et al. EGF receptor transactivation and PI3-kinase mediate stimulation of ERK by alpha(2A)-adrenoreceptor in intestinal epithelial cells: a role in wound healing. Eur J Pharmacol 2007 574 : 85 93.
44. Xu KP, Yin J, Yu FS. Lysophosphatidic acid promoting corneal epithelial wound healing by transactivation of epidermal growth factor receptor. Invest Ophthalmol Vis Sci 2007 48 : 636 43.
45. Xu KP, Riggs A, Ding Y et al. Role of ErbB2 in corneal epithelial wound healing. Invest Ophthalmol Vis Sci 2004 45 : 4277 83.
46. Miura Y, Yanagihara N, Imamura H et al. Hepatocyte growth factor stimulates proliferation and migration during wound healing of retinal pigment epithelial cells in vitro. Jpn J Ophthalmol 2003 47 : 268 75.