Three-dimensional imaging of the intact mouse cochlea by fluorescent laser scanning confocal microscopy

Hearing Research

Volumn 243, Issue 1-2, 2008, Pages 1-10

  MacDonald, Glen H ^a   Rubel, Edwin W ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Benzyl benzoate; Cochlea; Confocal; Fluorescence; Methyl salicylate; Refractive index

Indexed keywords

BENZYL BENZOATE; SALICYLIC ACID METHYL ESTER;

ANATOMY; ANIMAL TISSUE; ARTICLE; COCHLEA; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; LASER MICROSCOPY; MICROSCOPE IMAGE; MOUSE; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

ANIMALS; BENZOATES; COCHLEA; FLUORESCENT DYES; HAIR CELLS, AUDITORY, INNER; HAIR CELLS, AUDITORY, OUTER; HISTOLOGICAL TECHNIQUES; IMAGING, THREE-DIMENSIONAL; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED CBA; MICROSCOPY, CONFOCAL; MODELS, ANATOMIC; SALICYLATES;

EID: 49749098391     PISSN: 03785955     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.heares.2008.05.009     Document Type: Article

References (31)

1. Angelborg C., and Engstrom H. Supporting elements in the organ of Corti. I. Fibrillar structures in the supporting cells of the organ of Corti in mammals. Acta Otolaryngol. 301 Suppl. (1972) 49-56
2. Benveniste H., and Blackband S. MR microscopy and high resolution small animal MRI: applications in neuroscience research. Prog. Neurobiol. 67 (2002) 393-420
3. Berglund A.M., and Ryugo D.K. Hair cell innervation by spiral ganglion neurons in the mouse. J. Comp. Neurol. 255 (1987) 560-570
4. Berglund A.M., and Ryugo D.K. Neurofilament antibodies and spinal ganglion neurons of the mammalian cochlea. J. Comp. Neurol. 306 (1991) 393-408
5. Born M., and Wolf E. Principles of Optics. seventh ed. (2005), Cambridge, New York
6. Carlsson K. The influence of specimen refractive index, detector signal integration, and non-uniform scan speed on the imaging properties in confocal microscopy. J. Microsc. 163 (1991) 167-178
7. Celio M.R. Cabindin D-28k and parvalbumin in the rat nervous system. Neuroscience 35 (1990) 375-475
8. Egner A., and Hell S.W. Aberrations in confocal and multi-photon fluorescent microscopy induced by refractive index mismatch. In: Pawley J.B. (Ed). Handbook of Biological Confocal Microscopy. third ed. (2006), Springer, New York 404-441
9. Ellis E.A. Solutions to the problem of substitution of ERL 4221 for vinyl cyclohexene dioxide in Spurr low viscosity embedding formulations. Microsc. Today 14 (2006) 32
10. Hallworth R., and Ludeuna R.F. Differential expression of β tubulin isotypes in the adult gerbil cochlea. Hear. Res. 148 (2000) 161-172
11. Hardie N.A., MacDonald G., and Rubel E.W. A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy. Brain Res. 1000 (2004) 200-210
12. Hashimoto S., Kimura R.S., and Takasaka T. Computer-aided three-dimensional reconstruction of the inner hair cells and their nerve endings in the guinea pig cochlea. Acta Otolaryngol. 109 (1990) 228-234
13. Hell S., Reiner G., Cremer C., and Stelzer E.H.K. Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J. Microsc. 169 (1993) 391-405
14. Hibbs A.R., MacDonald G., and Garsha K. Practical confocal microscopy. In: Pawley J.B. (Ed). Handbook of Biological Confocal Microscopy. third ed. (2006), Springer, New York 650-671
15. Huang L.-C., Thorne P.R., Housley G.D., and Montegomery J.M. Spatiotemporal definition of neurite outgrowth, refinement and retraction in the developing mouse cochlea. Development 134 (2007) 2925-2933
16. Keller H.E. Objective lenses for confocal microscopy. In: Pawley J.B. (Ed). Handbook of Biological Confocal Microscopy. third ed. (2006), Springer, New York 154-161
17. Liberman M.C., Dodds L.W., and Pierce S. Afferent and efferent innervation of the cat cochlea: quantitative analysis with light and electron microscopy. J. Comp. Neurol. 301 (1990) 443-460
18. Maison S.F., Adams J.C., and Liberman M.C. Olivocochlear innervation in the mouse: immunocytochemical maps, crossed versus uncrossed contributions and transmitter colocalization. J. Comp. Neurol. 455 (2003) 406-416
19. A receptor subunits α1, α2, α5, α6, ß2, ß3, or delta. J. Neurosci. 26 (2006) 10315-10326
20. Munoz T.E., Giberson R.T., Demaree R., and Day J.R. Microwave-assisted immunostaining: a new approach yields fast and consistent results. J. Neurosci. Meth. 137 (2004) 133-139
21. Richardson K.C., Jarret L., and Finke E.H. Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Tech. 35 (1960) 313-323
22. Sato M., Henson M.M., Henson O.W., and Smith D.W. The innervation of outer hair cells: 3D reconstruction from TEM serial sections in the Japanese macaque. Hear. Res. 135 (1999) 29-38
23. Slepecky N., and Ulfendahl M. Actin-binding and microtuble-associated proteins in the organ of Corti. Hear. Res. 57 (1992) 201-215
24. Slepecky N., Henderson C.G., and Saha S. Post-translational modifications of tubulin suggest that dynamic microtubules are present in sensory cells and stable microtubules are present in supporting cells of the mammalian cohlea. Hear. Res. 91 (1995) 136-147
25. Spalteholz W. Über das Durchsichtigmachen von menschlichen und tierischen Präparaten und seine theoretischen Bedingungen (1914), Hirzel, Leipzig
26. Tinling S.P., Giberson R.T., and Kullar R.S. Microwave exposure increases demineralization rate independent of temperature. J. Microsc. 215 (2004) 230-235
27. Torok P., Hewlett S.J., and Varga P. The role of specimen-induced spherical aberration in confocal microscopy. J. Microsc. 188 (1997) 158-177
28. Voie A.H., Burns D.H., and Spelman F.A. Orthogonal-plane fluorescence optical sectioning: three-dimensional imaging of macroscopic biological specimens. J. Microsc. 170 (1993) 229-236
29. Voie A.H. Imaging the intact guinea pig tympanic bulla by orthogonal-plane fluorescence optical sectioning microscopy. Hear. Res. 171 (2002) 119-128
30. Voie, A.H, Saxon, G., Hess, M., 2007. OPFOS imaging of the cochlea. Abstract, 30th Midwinter Research Meeting of the Assoc. for Res. in Otolaryngology, February 10.
31. Wang G., and Vannier M. Micro-CT scanners for biomedical applications: an overview. Adv. Imag. 16 (2001) 18-27