Semi-quantitative analysis of Ruminococcus flavefaciens, Fibrobacter succinogenes and Streptococcus bovis in the equine large intestine using real-time polymerase chain reaction

British Journal of Nutrition

Volumn 100, Issue 3, 2008, Pages 561-568

  Hastie, Peter M ^a   Mitchell, Katherine ^b   Murray, Jo Anne M D ^b  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Bacteria; Horses; Intestine; Real time polymerase chain reaction

Indexed keywords

DNA; DNA 16S;

ANIMAL TISSUE; ARTICLE; BACTERIUM DETECTION; CECUM; COLON; DNA EXTRACTION; DNA SEQUENCE; FIBROBACTER SUCCINOGENES; FREEZE DRYING; HORSE; LARGE INTESTINE; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECTUM; RUMINOCOCCUS FLAVEFACIENS; STREPTOCOCCUS BOVIS;

ANIMALS; CECUM; COLON; FECES; FIBROBACTER; HORSES; INTESTINE, LARGE; RECTUM; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RUMINOCOCCUS; STREPTOCOCCUS BOVIS;

BACTERIA (MICROORGANISMS); EQUIDAE; FIBROBACTER SUCCINOGENES; RUMINOCOCCUS FLAVEFACIENS; STREPTOCOCCUS BOVIS;

EID: 49649124711     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S0007114508968227     Document Type: Article

References (33)

1. Hainze MTM, Muntifering RB & McCall CA (2003) Fibre digestion in horses fed typical diets with and without exogenous fibrolytic enzymes. J Equine Vet Sci 23, 111-115.
2. Glinsky MJ, Smith RM, Spires HR & Davis L (1976) Measurement of volatile fatty acid production rates in the caecum of the pony. J Anim Sci 42, 1465-1470.
3. McLean BML, Hyslop JJ, Longland AC, Cuddeford D & Hollands T (2000), Physical processing of barley and its effects on intra-caecal fermentation parameters in ponies. Anim Feed Sci Technol 85, 79-87.
4. Carroll CL, Hazard G, Coloe PJ & Hooper PT (1987) Laminitis and possible enterotoxaemia associated with carbohydrate overload in mares. Equine Vet J 19, 344-346.
5. Clarke LL, Roberts MC & Argenzio RA (1990) Feeding and digestive problems in horses: Physiologic responses to a concentrate meal. Vet Clin N Am Equine 6, 433-451.
6. Garner HE, Hutcheson DP, Coffman JR & Hahn AW (1977) Lactic acidosis: A factor associated with equine laminitis. J. Anim Sci 45, 1037-1041.
7. Rowe JB, Lees MJ & Pethick DW (1994) Prevention of acidosis and laminitis associated with grain feeding in horses. J Nutr 124 2742S-2744S.
8. Tajima K, Aminov RI, Nagamine T, Ogata K, Nakamura M, Matsui H & Benno Y (1999) Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol Ecol 29, 159-169.
9. Wilson KH & Blitchington RB (1996) Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 62, 2273-2278.
10. Pryde SE, Richardson AJ, Stewart CS & Flint HJ (1999) Molecular analysis of the microbial diversity present in the colonic wall, colonic lumen and caecal lumen of a pig. Appl Environ Microbiol 65, 5372-5377.
11. Daly K, Stewart CS, Flint HJ, Soraya P & Shirazi-Beechey SP (2001) Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes. FEMS Microbiol Ecol 38, 141-151.
12. Halliwell REW, Fleischman JB, Mackay-Smith M, Beech J & Gunson DE (1979) The role of allergy in chronic pulmonary disease of horses. J Am Vet Med Assoc 174, 277-281.
13. Nadkarni MA, Martin FE, Jacques NA & Hunter N (2002) Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148, 257-266.
14. Julliand V, de Vaux A, Millet L & Fonty G (1999) Identification of Ruminococcus flavefaciens as the predominant cellulolytic bacterial species of the equine cecum. Appl Environ Microbiol 65, 3738-3741.
15. Daly K, Soraya P & Shirazi-Beechey SP (2003) Design and evaluation of group-specific oligonucleotide probes for quantitative analysis of intestinal ecosystems: Their application to assessment of equine colonic microflora. FEMS Microbiol Ecol 44, 243-252.
16. Tajima K, Aminov RI, Nagamine T, Matsui H, Nakamura M & Benno Y (2001) Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl Environ Microbiol 67, 2766-2774.
17. Haarman M & Knol J (2005) Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula. Appl Environ Microbiol 71, 2318-2324.
18. Milinovich GJ, Trott DJ, Barrell AW, Thoefner MB, Blackall LL, AI Jassim RAM, Morton JM & Pollitt CC (2006) Changes in equine hindgut bacterial population during oligofructose-induced laminitis. Environ Microbiol 8, 885-898.
19. Applied Biosystems (2001) User Bulletin 2: Relative Quantilation of Gene Expression, pp. 1-36. Warrington: Applied Biosystems.
20. Whitehead TR & Cotta MA (1993) Development of a DNA probe for Streptocaccus bovis by using a cloned amylase gene. J Clin Microbiol 31, 2387-2391.
21. Michalet-Doreau B, Fernandez I, Peyron C & Millet L (2001) Fibrolytic activities and cellulolytic bacterial community structure in soli d and liquid phases of rumen contents. Reprod Nutr Dev 41, 187-194.
22. de Fombelle A, Julliand V, Drogoul C & Jacotot E (2001) Feeding and microbial disorders in horses: 1 - Effects of an abrupt incorporation of two levels of barley in a hay diet on, microbial profile and activities. J Equine Vet Sci 21, 439-445.
23. Lin CZ & Stahl DA (1995) Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations. Appl Environ Microbiol 61, 1348-1352.
24. Koike S, Shingu Y, Inaba H, Kawai M, Kobayashi Y, Hata H, Tanaka K & Okubo M (2000) Faecal bacteria in Hokkaido native horses as characterised by microscopic enumeration and competitive polymerase chain reaction assays. J Anim Eq Sci 11, 45-50.
25. de Fombelle A, Varloud M, Goachet AG, Jacotot E, Philippeau C, Drogoul C & Julliand V (2003) Characterisation of the microbial and biochemical profile of different segments of the digestive tract in horses given two distinct diets. Anim Sci 77, 293-304.
26. Al Jassim RAM & Rowe JB (1999) A better understanding of acidosis and its control. Rec Adv Anim Nutr Aust 12, 91-96.
27. Russell JB & Robinson PH (1984) Composition and characteristics of strains of Streptococcus bovis. J Dairy Sci 67, 1525-1531.
28. Cotta MA (1988) Amylolytic activity of selected species of ruminal bacteria. Appl Environ Microbiol 54, 772-776.
29. Whitehead TR & Cotta MA (2000) Development of molecular methods for identification of Streptococcus bovis from human and ruminal origins. FEMS Microbiol Lett 182, 237-240.
30. Al Jassim RAM, Scott PT, Trebbin AL, Trott D & Pollitt CC (2005) The genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract. FEMS Microbiol Lett 248, 75-81.
31. Dubrow RS, Edberg S, Wikfors E, Callan D, Troncalle F, Vender R, Brand M & Yapp R (1991) Faecal carriage of Streptococcus bovis and colorectal adenomas. Gastroenterology 101, 721-725.
32. Awada A, Van der Auwera P, Meunier F, Daneau D & Klastersky J (1992) Streptococcal and enterococcal bacterium in patients with cancer. Clin Infect Dis 15, 33-48.
33. Harley W (1992) Streptococcus bovis meningitis associated with colonic cillous adenoma. Clin Infect Dis 14, 979-980.