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The half-crossover can be viewed as a simplified Holliday-junction analog, which utilizes one strand, rather than the normal two strands, at the crossover exchange point. A similar structure was previously used in constructing DNA nanotubes (14).
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The half-crossover can be viewed as a simplified Holliday-junction analog, which utilizes one strand, rather than the normal two strands, at the crossover exchange point. A similar structure was previously used in constructing DNA nanotubes (14).
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Due to the modularity and standardization of the motif, assigning the dimensions of all the green domains in the lattice also uniquely determines the dimensions of all the other domains
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Due to the modularity and standardization of the motif, assigning the dimensions of all the green domains in the lattice also uniquely determines the dimensions of all the other domains.
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Materials and methods are available as supporting material on Science Online.
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The authors thank E. Winfree at Caltech for generously hosting the majority part of this work in his lab. For inspiring discussions, the authors thank E. Winfree, P. W. K. Rothemund, R. Schulman, V. A. Beck, J. R. Vieregg, R. D. Barish, B. Yurke, D. Y. Zhang, N. A. Pierce, S. Hamada, M. Bockrath, H. Maune, Y. Huang, C. R. Calvert, N. L. Dabby, and J. Kim. The authors are also grateful to N. A. Pierce at Caltech and J. Liu at Duke for facility support, to the Pierce group for the use of the unpublished DNA sequence design component and DNA structure illustration component of the NUPACK server www.nupack.org, and to B. Walters for technical assistance. The fluorescence microscope was built by R.F.H. and B. Yurke. There is a patent pending on this work. This work is supported by the Center for Biological Circuit Design at Caltech, NSF grants CCF-0523555 and CCF-0432038 to J.H.R, NSF grant CBET-0508284 to T.H.L, NSF grants 0622254 and 0432193 to E. Winfree, and NSF grant 0506468 to N
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The authors thank E. Winfree at Caltech for generously hosting the majority part of this work in his lab. For inspiring discussions, the authors thank E. Winfree, P. W. K. Rothemund, R. Schulman, V. A. Beck, J. R. Vieregg, R. D. Barish, B. Yurke, D. Y. Zhang, N. A. Pierce, S. Hamada, M. Bockrath, H. Maune, Y. Huang, C. R. Calvert, N. L. Dabby, and J. Kim. The authors are also grateful to N. A. Pierce at Caltech and J. Liu at Duke for facility support, to the Pierce group for the use of the unpublished DNA sequence design component and DNA structure illustration component of the NUPACK server (www.nupack.org), and to B. Walters for technical assistance. The fluorescence microscope was built by R.F.H. and B. Yurke. There is a patent pending on this work. This work is supported by the Center for Biological Circuit Design at Caltech, NSF grants CCF-0523555 and CCF-0432038 to J.H.R., NSF grant CBET-0508284 to T.H.L., NSF grants 0622254 and 0432193 to E. Winfree, and NSF grant 0506468 to N. A. Pierce.
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