Esterase autodisplay: Enzyme engineering and whole-cell activity determination in microplates with pH sensors

Applied and Environmental Microbiology

Volumn 74, Issue 15, 2008, Pages 4782-4791

  Schultheiss, Eva ^a   Weiss, Svenja ^b   Winterer, Elisa ^a   Maas, Ruth ^a   Heinzle, Elmar ^b   Jose, Joachim ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CELL MEMBRANES; CELLS; COLLOIDS; CYTOLOGY; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; FOOD ADDITIVES; GELATION; GELS; NUCLEIC ACIDS; OPTICAL SENSORS; ORGANIC ACIDS; PAINTING; PH; PH EFFECTS; PH SENSORS; POLYMERS; POLYSACCHARIDES; PORT TERMINALS; SENSORS; SODIUM; SODIUM SULFATE; SUBSTRATES; SULFATE MINERALS; TECHNOLOGY;

BACTERIAL ENZYMES; C-TERMINAL EXTENSION; CATALYTIC DOMAINS; CELL ACTIVITIES; CELL ENVELOPES; E. COLI; ENZYME ENGINEERING; ESTERASE ACTIVITIES; HYDROLASE; MICROPLATES; MULTIPLE SEQUENCE ALIGNMENT; OUTER MEMBRANES; PERIPLASMIC; PH-DEPENDENT; PH-SENSOR; PLATFORM TECHNOLOGY; PROTEASE DIGESTION; SALMONELLA ENTERICA SEROVAR TYPHIMURIUM; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SURFACE EXPOSURE; WHOLE CELLS;

ENZYME SENSORS;

1 NAPHTHYL ACETATE; ASPARAGINE; BACTERIAL ENZYME; GLYCINE; HISTIDINE; HYDROLASE; PROTEIN APEE; PROTEIN ESJA; PROTEINASE; SERINE; SERINE PROTEINASE; TRIACYLGLYCEROL LIPASE;

AGAR; BACTERIUM; BIOENGINEERING; ELECTROKINESIS; ENZYME ACTIVITY; GEL; MICROPLATE; PH; POLYMERASE CHAIN REACTION; SENSOR;

ARTICLE; BIOENGINEERING; BIOSENSOR; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL ACTIVITY; CELL SURFACE; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CYTOPLASM; DISPLAY SYSTEM; DNA SEQUENCE; ENVELOPE GENE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME METABOLISM; ESCHERICHIA COLI; GEL; GENE AMPLIFICATION; GENE FUSION; GENE OVEREXPRESSION; GENE TRANSLOCATION; GENETIC VARIABILITY; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTER MEMBRANE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN TRANSPORT; SALMONELLA ENTERICA; SEQUENCE ALIGNMENT; SEROTYPE;

AMINO ACID SEQUENCE; CONSERVED SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ESTERASES; GENETIC ENGINEERING; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 49449092134     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01575-07     Document Type: Article

References (49)

1. Akoh, C. C., G. C. Lee, Y. C. Liaw, T. H. Huang, and J. F. Shaw. 2004. GDSL family of serine esterases/lipases. Prog. Lipid Res. 43:534-552.
2. Akoh, C. C., G. C. Lee, and J. F. Shaw. 2004. Protein engineering and applications of Candida rugosa lipase isoforms. Lipids 39:513-526.
3. Becker, S., A. Michalczyk, S. Wilhelm, K. E. Jaeger, and H. Kolmar. 2007. Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surface. Chembiochem 8:943-949.
4. Becker, S., S. Theile, N. Heppeler, A. Michalczyk, A. Wentzel, S. Wilhelm, K. E. Jaeger, and H. Kolmar. 2005. A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS Lett. 579:1177-1182.
5. Bessette, P. H., J. J. Rice, and P. S. Daugherty. 2004. Rapid isolation of high-affinity protein binding peptides using bacterial display. Protein Eng. Des. Sel. 17:731-739.
6. Bornscheuer, U. T. 2002. Microbial carboxyl esterases: classification, properties and application in biocatalysis. FEMS Microbiol. Rev. 26:73-81.
7. Carinato, M. E., P. Collin-Osdoby, X. Yang, T. M. Knox, C. A. Conlin, and C. G. Miller. 1998. The apeE gene of Salmonella typhimurium encodes an outer membrane esterase not present in Escherichia coli. J. Bacteriol. 180:3517-3521.
8. Dane, K. Y., L. A. Chan, J. J. Rice, and P. S. Daugherty. 2006. Isolation of cell specific peptide ligands using fluorescent bacterial display libraries. J. Immunol. Methods 309:120-129.
9. Daugherty, P. S., B. L. Iverson, and G. Georgiou. 2000. Flow cytometric screening of cell-based libraries. J. Immunol. Methods 243:211-227.
10. Drepper, T., T. Eggert, W. Hummel, C. Leggewie, M. Pohl, F. Rosenau, S. Wilhelm, and K. E. Jaeger. 2006. Novel biocatalysts for white biotechnology. Biotechnol. J. 1:777-786.
11. Emanuelsson, O., S. Brunak, G. von Heijne, and H. Nielsen. 2007. Locating proteins in the cell using TargetP, SignalP and related tools. Nat. Protoc. 2:953-971.
12. Francisco, J. A., and G. Georgiou. 1994. The expression of recombinant proteins on the external surface of Escherichia coli. Biotechnological applications. Ann. N. Y. Acad. Sci. 745:372-382.
13. Georgiou, G., C. Stathopoulos, P. S. Daugherty, A. R. Nayak, B. L. Iverson, and R. Curtiss III. 1997. Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat. Biotechnol. 15:29-34.
14. Gombert, F. O., R. Schaude, W. Troeger, G. Jung, B. H. W. Ruebsamen-Waigmann, S. Pfeifer, K. Krohn, M. Taehtinen, V. Ovod, A. Ranki, L. Shi, and P. Wernet. 1990. Complete epitope fine-mapping of HIV-1 Nef-protein, p. 827-875. In E. Giralt and D. Andreu (ed.), Peptides. Escom Science Publishers, Leiden, The Netherlands.
15. Hall, S. S., S. Mitragotri, and P. S. Daugherty. 2007. Identification of peptide ligands facilitating nanoparticle attachment to erythrocytes. Biotechnol. Prog. 23:749-754.
16. Hantke, K. 1981. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol. Gen. Genet. 182:288-292.
17. Jaeger, K. E., and M. T. Reetz. 1998. Microbial lipases form versatile tools for biotechnology. Trends Biotechnol. 16:396-403.
18. Jahnig, F. 1990. Structure predictions of membrane proteins are not that bad. Trends Biochem. Sci. 15:93-95.
19. John, G. T., D. Goelling, I. Klimant, H. Schneider, and E. Heinzle. 2003. pH-sensing 96-well microtitre plates for the characterization of acid production by dairy starter cultures. J. Dairy Res. 70:327-333.
20. John, G. T., and E. Heinzle. 2001. Quantitative screening method for hydrolases in microplates using pH indicators: determination of kinetic parameters by dynamic pH monitoring. Biotechnol. Bioeng. 72:620-627.
21. Jose, J. 2006. Autodisplay: efficient bacterial surface display of recombinant proteins. Appl. Microbiol. Biotechnol. 69:607-614.
22. Jose, J., R. Bernhardt, and F. Hannemann. 2002. Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli. J. Biotechnol. 95:257-268.
23. Jose, J., and S. Handel. 2003. Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry. Chembiochem 4:396-405.
24. Jose, J., F. Hannemann, and R. Bernhardt. 2001. Functional display of active bovine adrenodoxin on the surface of E. coli by chemical incorporation of the [2Fe-2S] cluster. Chembiochem 2:695-701.
25. Jose, J., F. Jahnig, and T. F. Meyer. 1995. Common structural features of IgA1 protease-like outer membrane protein autotransporters. Mol. Microbiol. 18:378-380.
26. Jose, J., and T. F. Meyer. 2007. The autodisplay story, from discovery to biotechnical and biomedical applications. Microbiol. Mol. Biol. Rev. 71:600-619.
27. Jose, J., and S. von Schwichow. 2004. Autodisplay of active sorbitol dehydrogenase (SDH) yields a whole cell biocatalyst for the synthesis of rare sugars. Chembiochem 5:100-108.
28. Jose, J., and S. von Schwichow. 2004. "Cystope tagging" for labeling and detection of recombinant protein expression. Anal. Biochem. 331:267-274.
29. Kumar, S., C. Wittmann, and E. Heinzle. 2004. Minibioreactors. Biotechnol. Lett. 26:1-10.
30. Lee, S. Y., J. H. Choi, and Z. Xu. 2003. Microbial cell-surface display. Trends Biotechnol. 21:45-52.
31. Maurer, J., J. Jose, and T. F. Meyer. 1997. Autodisplay: one-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli. J. Bacteriol. 179:794-804.
32. Maurer, J., J. Jose, and T. F. Meyer. 1999. Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions. J. Bacteriol. 181:7014-7020.
33. Reetz, M. T. 2000. Evolution in the test tube as a means to create enantioselective enzymes for use in organic synthesis. Sci. Prog. 83:157-172.
34. Reetz, M. T., and L. W. Wang. 2006. High-throughput selection system for assessing the activity of epoxide hydrolases. Comb. Chem. High Throughput Screen. 9:295-299.
35. Reetz, M. T., S. Wilensek, D. Zha, and K. E. Jaeger. 2001. Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis. Angew. Chem. Int. Ed. Engl. 40:3589-3591.
36. Reiter, B., A. Glieder, D. Talker, and H. Schwab. 2000. Cloning and characterization of EstC from Burkholderia gladioli, a novel-type esterase related to plant enzymes. Appl. Microbiol. Biotechnol. 54:778-785.
37. Schmidinger, H., R. Birner-Gruenberger, G. Riesenhuber, R. Saf, H. Susani-Etzerodt, and A. Hermetter. 2005. Novel fluorescent phosphonic acid esters for discrimination of lipases and esterases. Chembiochem 6:1776-1781.
38. Schultheiss, E., C. Paar, H. Schwab, and J. Jose. 2002. Functional esterase surface display by the autotransporter pathway in Escherichia coli. J. Mol. Catal. B 18:89-97.
39. Sergeeva, A., M. G. Kolonin, J. J. Molldrem, R. Pasqualini, and W. Arap. 2006. Display technologies: application for the discovery of drug and gene delivery agents. Adv. Drug Deliv. Rev. 58:1622-1654.
40. Surrey, T., A. Schmid, and F. Jahnig. 1996. Folding and membrane insertion of the trimeric beta-barrel protein OmpF. Biochemistry 35:2283-2288.
41. Susani-Etzerodt, H., H. Schmidinger, G. Riesenhuber, R. Birner-Gruenberger, and A. Hermetter. 2006. A versatile library of activity-based probes for fluorescence detection and/or affinity isolation of lipolytic enzymes. Chem. Phys. Lipids 144:60-68.
42. Talker-Huiber, D., J. Jose, A. Glieder, M. Pressnig, G. Stubenrauch, and H. Schwab. 2003. Esterase EstE from Xanthomonas vesicatoria (Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters. Appl. Microbiol. Biotechnol. 61:479-487.
43. Ueda, M., and A. Tanaka. 2000. Genetic immobilization of proteins on the yeast cell surface. Biotechnol. Adv. 18:121-140.
44. Vogel, H., and F. Jahnig. 1986. Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methods. J. Mol. Biol. 190:191-199.
45. Wang, H., and B. C. Dowds. 1993. Phase variation in Xenorhabdus luminescens: cloning and sequencing of the lipase gene and analysis of its expression in primary and secondary phases of the bacterium. J. Bacteriol. 175:1665-1673.
46. Weiss, S., G. T. John, I. Klimant, and E. Heinzle. 2002. Modeling of mixing in 96-well microplates observed with fluorescence indicators. Biotechnol. Prog. 18:821-830.
47. Wernerus, H., and S. Stahl. 2004. Biotechnological applications for surface-engineered bacteria. Biotechnol. Appl. Biochem. 40:209-228.
48. Wilhelm, S., J. Tommassen, and K. E. Jaeger. 1999. A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa. J. Bacteriol. 181:6977-6986.
49. Yeung, Y. A., and K. D. Wittrup. 2002. Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture. Biotechnol. Prog. 18:212-220.