Phenylalanine ammonia lyase functions as a switch directly controlling the accumulation of calycosin and calycosin-7-O-β-D-glucoside in Astragalus membranaceus var. mongholicus plants

Journal of Experimental Botany

Volumn 59, Issue 11, 2008, Pages 3027-3037

  Pan, Haiyun ^a   Wang, Yuguo ^a   Zhang, Yongfeng ^b   Zhou, Tongshui ^a   Fang, Changming ^a   Nan, Peng ^a   Wang, Xiaoqiang ^c   Li, Xiaobing ^d   Wei, Yinlong ^a   Chen, Jiakuan ^a  

^a FUDAN UNIVERSITY   (China)

^b ANHUI AGRICULTURAL UNIVERSITY   (China)

^c PLANT BIOLOGY DIVISION   (United States)

^d ANHUI UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Accumulation; Enzyme inhibitor; Gene switch; Isoflavonoid; Phenylalanine ammonia lyase

Indexed keywords

7,3' DIHYDROXY 4' METHOXYISOFLAVONE; 7,3'-DIHYDROXY-4'-METHOXYISOFLAVONE; ALCOHOL DEHYDROGENASE; ALPHA AMINOOXYHYDROCINNAMIC ACID; ALPHA-AMINOOXY-BETA-PHENYLPROPIONIC ACID; CALYCOSIN 7 O BETA D GLUCOSIDE; CALYCOSIN-7-O-BETA-D-GLUCOSIDE; CHALCONE ISOMERASE; CHALCONE REDUCTASE; CINNAMATE 4 MONOOXYGENASE; COMPLEMENTARY DNA; DRUG DERIVATIVE; GLUCOSIDE; ISOFLAVONE DERIVATIVE; ISOMERASE; MESSENGER RNA; PHENYLALANINE; PHENYLALANINE AMMONIA LYASE;

ARTICLE; ASTRAGALUS PLANT; BIOSYNTHESIS; COLD; DRUG ANTAGONISM; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; LIGHT; METABOLISM;

ALCOHOL OXIDOREDUCTASES; ASTRAGALUS PLANT; COLD; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, PLANT; GLUCOSIDES; INTRAMOLECULAR LYASES; ISOFLAVONES; LIGHT; PHENYLALANINE; PHENYLALANINE AMMONIA-LYASE; RNA, MESSENGER; TRANS-CINNAMATE 4-MONOOXYGENASE;

ASTRAGALUS MEMBRANACEUS; ASTRAGALUS MEMBRANACEUS VAR. MONGHOLICUS;

EID: 49449090414     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/ern152     Document Type: Article

References (37)

1. Ali-Benali MA, Alary R, Joudrier P, Gautier MF. 2005. Comparative expression of five Lea genes during wheat seed development and in response to abiotic stresses by real-time quantitative RT-PCR. Biochimica et Biophysica Acta - Gene Structure and Expression 1730, 56-65.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
3. Choi S, Park SR, Heo TR. 2005. Inhibitory effect of astragali radix on matrix degradation in human articular cartilage. Journal of Microbiology and Biotechnology 15, 1258-1266.
4. Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. The Plant Cell 7, 1085-1097.
5. Fan Y, Wu DZ, Gong YQ, Zhou RY, Hu ZB. 2003. Effects of calycosin on the impairment of barrier function induced by hypoxia in human umbilical vein endothelial cells. European Journal of Pharmacology 481, 33-40.
6. Graham TL, Graham MY. 1996. Signaling in soybean phenylpropanoid responses: dissection of primary, secondary, and conditioning effects of light, wounding, and elicitor treatments. Plant Physiology 110, 1123-1133.
7. Hahlbrock K, Scheel D. 1989. Physiology and molecular biology of phenylpropaniod metabolism. Annual Review of Plant Physiology and Plant Molecular Biology 40, 347-369.
8. Hakamatsuka T, Mori K, Ishida S, Ebizuka Y, Sankawa U. 1998. Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of Pueraria lobata. Phytochemistry 49, 497-505.
9. Haughn G, Somerville C. 1986. Sulfonylurea-resistant mutants of Arabidopsis thaliana. Molecular & General Genetics 204, 430-434.
10. Heller W, Forkmann G. 1994. Biosynthesis of flavonoids. In: Harborne JB, ed. The flavonoids: advances in research since 1986. London: Chapman and Hall, 499-535.
11. Joung JY, Kasthuri GM, Park JY, Kang WJ, Kim HS, Yoon BS, Joung H, Jeon JH. 2003. An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5′-deoxyflavonoids in transgenic tobacco. Biochemical and Biophysical Research Communication 303, 326-331.
12. Ke D, Saltveit ME. 1989. Carbon dioxide-induced brown stain development as related to phenolic metabolism in iceberg lettuce. Journal of the American Society for Horticultural Science 114, 789-794.
13. Kessmann H, Edwards R, Geno PW, Dixon RA. 1990. Stress responses in alfalfa Medicago sativa L.V. Consitutive and elicitor-induced accumulation of isoflavanoid conjugates in cell suspension cultures. Plant Physiology 94, 227-232.
14. Kubasek WL, Ausubel FM, Shirley BW. 1998. A light-independent developmental mechanism potentiates flavonoid gene expression in Arabidopsis seedlings. Plant Molecular Biology 37, 217-223.
15. Lee YM, Choi SI, Lee JW, Jung SM, Park SM, Heo TR. 2005. Isolation of hyaluronidase inhibitory component from the roots of Astraglus membranaceus Bunge Astragali radix. Food Science and Biotechnology 14, 263-267.
16. Leyva A, Jarillo JA, Salinas J, Martinezzapater JM. 1995. Low-temperature induces the accumulation of phenylalanine ammonialyase and chalcone synthase messenger-RNAs of Arabidopsis thaliana in a light-dependent manner. Plant Physiology 108, 39-46.
17. Liu CJ, Huhman D, Sumner LW, Dixon RA. 2003. Regiospecific hydroxylation of isoflavones by cytochrome P450 81E enzymes from Medicago truncatula. The Plant Journal 36, 471-484.
18. Liu RR, Xu SH, Li JL, Hu YL, Lin ZP. 2006. Expression profile of a PAL gene from Astragalus membranaceus var. mongholicus and its crucial role in flux into flavonoid biosynthesis. Plant Cell Reports 25, 705-710.
19. Lozovaya VV, Lygin AV, Zernova OV, Li S, Hartman GL, Widholm JM. 2004. Isoflavonoid accumulation in soybean hairy roots upon treatment with Fusarium solani. Plant Physiology and Biochemistry. 42, 671-679.
20. McCallum JA, Walker JRL. 1990. Phenolic biosynthesis during grain development in wheat changes in phenylalanine ammonia lyase activity and soluble phenolic content. Journal of Cereal Science 11, 35-49.
21. Ma XQ, Duan JA, Zhu DY, Dong TTX, Tsim KWK. 2000. Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain. Phytochemistry 54, 363-368.
22. Ma XQ, Shi Q, Duan JA, Dong TTX, Tsim KWK. 2002. Chemical analysis of Radix Astragali Huangqi in China: a comparison with its adulterants and seasonal variations. Journal of Agricultural and Food Chemistry 50, 4861-4866.
23. Nakamura T, Hashimoto A, Nishi H, Kokusenya Y. 1999. Investigation on the marker substances of crude drugs in formulations. I. Marker substances for the identification of Astragali Radix in kampo and drinkable preparations. Yakugaku Zasshi 119, 391-400.
24. Pan HY, Fang CM, Zhou TS, Wang QZ, Chen JK. 2007. Accumulation of calycosin and its 7-O-β-D-glucoside and related gene expression in seedlings of Astragalus membranaceus Bge. var. mongholicus Bge. Hsiao induced by low temperature stress. Plant Cell Reports 26, 1111-1120.
25. Peiser G, Lopez-Galvez G, Cantwell M, Saltveit ME. 1998. Phenylalanine ammonia lyase inhibitors control browning of cut lettuce. Postharvest Biology and Technology 14, 171-177.
26. Philipps G, Drzewiecki C, Barth O, Zschiesche W, Humbeck K. 2006. Light-dependent expression of the cold-regulated gene HvMC1 in barley (Hordeum vulgare l.). Journal of Thermal Biology 31, 473-482.
27. Ralston L, Subramanian S, Matsuno M, Yu O. 2005. Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II chalcone isomerases. Plant Physiology 137, 1375-1388.
28. Shimada N, Aoki T, Sato S, Nakamura Y, Tabata S, Ayabe S. 2003. A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxyisoflavonoids in Lotus japonicus. Plant Physiology 131, 941-951.
29. Tsukamoto C, Shimada S, Igita K, Kudou S, Kokubun M, Okubo K, Kitamura K. 1995. Factors affecting isoflavone content in soybean seeds - changes in isoflavones, saponins, and composition of fatty-acids at different temperatures during seed development. Journal of Agricultural and Food Chemistry 43, 1184-1192.
30. Wagner H, Bauer R, Xiao PG, Chen JM, Michler G. 1997. Radix Astragali Huang Qi. Chinese Drug Monographs and Analysis 1, 1-17.
31. Wang KLC, Li H, Ecker JR. 2002. Ethylene biosynthesis and signaling networks. The Plant Cell 14, S131-S151.
32. Werck-Reichhart D, Batard Y, Kochs G, Lesot A, Durst F. 1993. Monospecific polyclonal antibodies directed against purified cinnamate 4-hydroxylase from Helianthus tuberosus: immunopurification, immunoquantitation, and interspecies cross-reactivity. Plant Physiology 102, 1291-1298.
33. Wu T, Bligh SWA, Gu LH, Wang ZT, Liu HP, Cheng XM, Branford-White CJ, Hu ZB. 2005. Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV. Fitoterapia 76, 157-165.
34. Yu O, McGonigle B. 2005. Metabolic engineering of isoflavone biosynthesis. Advances in Agronomy 86, 147-190.
35. Yu O, Shi J, Hession AO, Maxwell CA, McGonigle B, Odell JT. 2003. Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63, 753-763.
36. Zheng XY. 2005. Pharmacopoeia of the Peoples Republic of China, Vol. 1, Chinese edn. Beijing: Chemical Industry Press, 212-213.
37. Zobayed SMA, Afreen F, Kozai T. 2005. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John's wort. Plant Physiology and Biochemistry 43, 977-984.