Comparison of an automated pattern analysis machine vision time-lapse system with traditional endpoint measurements in the analysis of cell growth and cytotoxicity

Alternatives to Laboratory Animals

Volumn 36, Issue 3, 2008, Pages 313-325

  Toimela, Tarja ^a   Tähti, Hanna ^a   Ylikomi, Timo ^a,b  

^a UNIVERSITY OF TAMPERE MEDICAL SCHOOL   (Finland)

^b TAMPERE UNIVERSITY HOSPITAL   (Finland)

Author keywords

Automated pattern analysis; Cultured cells; Cytotoxicity; Endpoint measurements; Machine vision

Indexed keywords

ARTICLE; ARTIFICIAL INTELLIGENCE; AUTOMATION; CELL CULTURE; CELL GROWTH; CELL STRUCTURE; COMPUTER AIDED DESIGN; COMPUTER PROGRAM; CYTOTOXICITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; INTERMETHOD COMPARISON; MACHINE VISION SYSTEM; PRIORITY JOURNAL; STAINING;

EID: 49449083548     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290803600309     Document Type: Article

References (21)

1. Batchelor, B.G. & Whelan, P.F. (1997). Intelligent Vision Systems for Industry, 457pp. London, UK: Springer.
2. Forsyth, D. & Ponce, J. (2003). Computer Vision. A modern approach, 693pp. Upper Saddle River, NJ, USA: Prentice Hall.
3. Snyder, W.E. & Qi, H. (2004). Machine Vision, 452pp. Cambridge, UK: Cambridge University Press.
4. Cook, J.A. & Mitchell, J.B. (1989). Viability measurements in mammalian cell systems. Analytical Biochemistry 179, 1-7.
5. Marshall, N.J., Goodwin, C.J. & Holt, S.J. (1995). A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth Regulation 5, 69-84.
6. Borenfreund, E. & Puerner, J.A. (1985). Toxicity determination in vitro by morphological alterations and neutral red absorption. Toxicology Letters 24, 119-124.
7. Brasaemle, D.L. & Attie, A.D. (1988). Microelisa reader quantitation of fixed, stained, solubilized cells in microtitre dishes. Biotechniques 6, 418-419.
8. Labarca, C. & Paigen, K. (1980). A simple, rapid, and sensitive DNA assay procedure. Analytical Biochemistry 102, 344-352.
9. Daxhelet, G.A., Coene, M.M., Hoet, P.P. & Cocito, C.G. (1989). Spectrofluorometry of dyes with DNAs of different base composition and conformation. Analytical Biochemistry 179, 401-403.
10. Saceda, M., Lippman, M.E., Chambon, P., Lindsey, R.L., Ponglikitmongkol, M., Puente, M. & Martin, M.B. (1988). Regulation of the estrogen receptor in MCF-7 cells by estradiol. Molecular Endocrinology 2, 1157-1162.
11. Vienonen, A., Miettinen, S., Manninen, T., Altucci, L., Wilhelm, E. & Ylikomi, T. (2003). Regulation of nuclear receptor and cofactor expression in breast cancer cell lines. European Journal of Endocrinology 148, 469-479.
12. Morse, D.L., Gray, H., Payne, C.M. & Gillies, R.J. (2005). Docetaxel induces cell death through mitotic catastrophe in human breast cancer cells. Molecular Cancer Therapeutics 4, 1495-1504.
13. Sarvilinna, N., Eronen, H., Miettinen, S., Vienonen, A. & Ylikomi, T. (2006). Steroid hormone receptors and coregulators in endocrine-resistant and estrogen-independent breast cancer cells. International Journal of Cancer 118, 832-840.
14. Ryan, M.J., Johnson, G., Kirk, J., Fuerstenberg, S.M., Zager, R.A. & Torok-Storb, B. (1994). HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney. Kidney International 45, 48-57.
15. Bär, P.R. (1996). Apoptosis - The cell's silent exit. Life Sciences 59, 369-378.
16. Fawthrop, D.J., Boobis, A.R. & Davies, D.S. (1991). Mechanisms of cell death. Archives of Toxicology 65, 437-444.
17. Chang, L.K., Putcha, G.V., Deshmukh, M. & Johnson, E.M., Jr (2002). Mitochondrial involvement in the point of no return in neuronal apoptosis. Biochimie 84, 223-231.
18. Nicotera, P., Leist, M. & Ferrando, M. (1999). Apoptosis and necrosis: different execution of the same death. Biochemical Society Symposium 66, 69-73.
19. Axelsson, V., Holback, S., Sjogren, M., Gustafsson, H. & Forsby, A. (2006). Gliotoxin induces caspase-dependent neurite degeneration and calpain-mediated general cytotoxicity in differentiated human neuroblastoma SH-SY5Y cells. Biochemical & Bio physical Research Communications 345, 1068-1074.
20. Nandi, A., Chandi, D., Lechesa, R., Pryor, S.C., McLaughlin, A., Bonventre, J.A., Flynn, K. & Weeks, B.S. (2006). Bifenthrin causes neurite retraction in the absence of cell death: A model for pesticide associated neurodegeneration. Medical Scientific Monitoring 12, BR169-BR173.
21. Sakai, Y., Shoji, R., Mishima, Y., Sakoda, A. & Suzuki, M. (2000). Rapid and sensitive neurotoxicity test based on the morphological changes of PC12 cells with simple computer-assisted image analysis. Journal of Bioscience & Bioengineering 90, 20-24.