Characterization of an Erwinia sp. isolated from necrotic pear blossoms in Valencia, Spain

Acta Horticulturae

Volumn 590, Issue , 2002, Pages 139-142

  Rosello M ^a   Garcia Vidal S ^a   Llop, P ^b   Gorris, M T ^b   Donat, V ^b   Penalver J ^b   Lopez M M ^b   Tarin A ^c   Chartier, R ^d   Paulin, J P ^d   Gardan, L ^d  

^a SC Sanidad Vegetal   (Spain)

^b INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^c CA la Turisana   (Spain)

^d CEMAGREF   (France)

Author keywords

Biochemical tests; E. amylovora; Inoculations; PCR; PFGE; Serology

Indexed keywords

EID: 4944242069     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2002.590.18     Document Type: Conference Paper

References (11)

1. Bereswill, S., Pahl, A., Bellemann, P., Berger, F., Zeller, W. and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by PCR analysis. Appl. Environ. Microbiol. 58: 3522-3526.
2. Bazzi, Merighi, M., López, M.M., Zhang, Y., Jock, S. and Geider, K. 1999. Differentiation of E. amylovora strains isolated in Southern Europe by PFGE analysis. Acta Hort. 489: 197-200.
3. Gorris, M.T., Camarasa, E., López, M.M., Paulin, K.P., Chartier, R. and Cambra, M. 1996. Production and characterization of monoclonal antibodies specific for Erwinia amylovora and their use in different serological techniques. Acta Hort. 411: 47-51.
4. Guilford, P.J., Taylor, R.K., Clark, R.G., Hale, C.N. and Forster, R.L.S. 1996. PCR-based techniques for the detection of Erwinia amylovora. Acta Hort. 411: 53-56.
5. Ishimaru, C. and Klos, E.J. 1984. New medium for detecting Erwinia amylovora and its use in epidemiological studies. Phytopathology 74: 1342-1345.
6. Kim, W.S., Gardan, L., Rhim, S.L. and Geider, K. 1999. Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Int. J. Syst. Bact, 49: 899-906.
7. King, E.O., Ward, M. and Raney, D.E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44: 301-307.
8. Llop, P., Bonatera, A., Peñalver, J. and López, M.M. 2000. Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material. Appl. Environ. Microbiol. 66: 2071-2078.
9. Maes, M., Garbeva, P. and Crepel, C. 1996. Identification and sensitive endophytic detection of the fire blight pathogen Erwinia amylovora with 23S ribosomal DNA sequences and the polymerase chain reaction. Plant Pathol. 45: 1139-1149.
10. Siverio, F. and López, M.M. 1996. Characteristics of the whole cell fatty acids profiles of Pseudomonas corrugata. Eur. J. Pl. Pathol. 192: 519-526.
11. Zhang, Y. and Geider, K. 1997. Differentiation of Erwinia amylovora strains by pulsed field gel electrophoresis. Appl. Environ. Microbiol. 63: 4421-4426.