A real-time quadriplex PCR assay for the diagnosis of rectal lymphogranuloma venereum and non-lymphogranuloma venereum Chlamydia trachomatis infections

Sexually Transmitted Infections

Volumn 84, Issue 4, 2008, Pages 273-276

  Chen, C Y ^a   Chi, K H ^a   Alexander, S ^b   Ison, C A ^b   Ballard, R C ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RIBONUCLEASE P;

ARTICLE; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CONFIDENCE INTERVAL; DIAGNOSTIC PROCEDURE; HUMAN; KAPPA STATISTICS; LYMPHOGRANULOMA VENEREUM; PERFORMANCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RECTUM; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS;

CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; HUMANS; LYMPHOGRANULOMA VENEREUM; MALE; POLYMERASE CHAIN REACTION; PROCTITIS; SENSITIVITY AND SPECIFICITY;

EID: 49049097891     PISSN: 13684973     EISSN: None     Source Type: Journal    
DOI: 10.1136/sti.2007.029058     Document Type: Article

References (19)

1. Jebbari H, Alexander S, Ward H, et al. Update on lymphogranuloma venereum in the united Kingdom. Sex Transm Infect 2007;83:324-6.
2. Chen CY, Chi KH, Alexander S, et al. The molecular diagnosis of lymphogranuloma venereum: evaluation of a real-time multiplex polymerase chain reaction test using rectal and urethral specimens. Sex Transm Dis 2007;34:451-5.
3. Morré SA, Spaargaren J, Fennema J, et al. Real-time polymerase chain reaction to diagnose lymphogranuloma venereum. Emerg Infect Dis 2005;11:1311-12.
4. Halse TA, Musser KA, Limberger RJ. A multiplex real-time PCR assay for rapid detection of Chlamydia trachomatis and identification of serovar L-2, the major cause of lymphogranuloma venereum in New York. Mol Cell Prob 2006;20:290-7.
5. Sturm P, Moodley P, Govender K, et al. Molecular diagnosis of lymphogranuloma venereum in patients with genital ulcer disease. J Clin Microbiol 2005;43:2973-5.
6. Lan J, Ossewaarde JM, Walboomers JMM, et al. Improved PCR sensitivity for direct genotyping of Chlamydia trachomatis serovars by using a nested PCR. J Clin Microbiol 1994;32:528-30.
7. Lan J, Walboomers JMM, Roosendaal R, et al. Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 1993;31:1060-5.
8. Yuan Y, Zhang Y-X, Watkins NG, et al. Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars. Infect Immun 1989;57:1040-9.
9. Black CM. Current methods of laboratory diagnosis of Chlamydia trachomatis infections. Clin Microbiol Rev 1997;10:160-84.
10. Van der Snoek E, Ossewaarde J, Van der Meijden W, et al. The use of serologic titers of IgA and IgG in (early) discrimination between rectal infection with non-LGV and LGV serovars of Chlamydia trachomatis. Sex Transm Infect 2007; 83:330-4.
11. Valassina M, Cusi MG, Corsaro D, et al. Detection by multiplex polymerase chain reaction and typing of Chlamydia trachomatis isolates. FEMS Microbiol Lett 1995;130:205-9.
12. Watson MW, Lambden PR, Clarke IN. Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. J Clin Microbiol 1991;29:1188-93.
13. Mutter GL, Boynton KA. PCR bias in amplification of androgen receptor alleles, a trinucleotide repeat marker used in clonality studies. Nucleic Acids Res 1995;23:1411-18.
14. Polz MF, Cavanaugh CM. Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol 1998;64:3724-30.
15. Walsh PS, Erlich HA, Higuchi R. Preferential PCR amplification of alleles: mechanisms and solutions. PCR Methods App 1992;1:241-50.
16. Ripa T, Nilsson PA. A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex Transm Dis 2007;34:255-6.
17. Farencena A, Comanducci A, Donati M, et al. Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma. Infect Immun 1997;65:2965-9.
18. Stothard DR, Williams JA, Van der Pol B, et al. Identification of a Chlamydia trachomatis serovar E urogenital isolate which lacks the cryptic plasmid. Infect Immun 1998;66:6010-13.
19. Magbanua JP, Goh TG, Michel CE, et al. Chlamydia trachomatis variant not detected by plasmid based nucleic acid amplification tests: molecular characterization and failure of single dose azithromycin. Sex Transm Infect 2007;83:339-43.