An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation

Analytical Biochemistry

Volumn 381, Issue 1, 2008, Pages 74-80

  Ono, Toshio ^a   Nemoto, Takayuki K ^a   Shimoyama, Yu ^b   Kimura, Shigenobu ^b   Ohara Nemoto, Yuko ^a  

^a NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES   (Japan)

^b IWATE MEDICAL UNIVERSITY   (Japan)

Author keywords

Autoproteolysis; Glutamyl endopeptidase; Prosequence; Staphylococcus epidermidis; Staphylococcus warneri

Indexed keywords

ESCHERICHIA COLI; RECOMBINANT PROTEINS; SOAPS (DETERGENTS);

AUTOPROTEOLYSIS; GLUTAMYL ENDOPEPTIDASE; PROSEQUENCE; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS WARNERI;

STAPHYLOCOCCUS AUREUS;

GLUTAMYL ENDOPEPTIDASE; PROTEINASE; RECOMBINANT PROTEIN; THERMOLYSIN;

AMINO ACID SUBSTITUTION; ARTICLE; CHIMERA; ESCHERICHIA COLI; GENE MUTATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; STAPHYLOCOCCUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS WARNERI;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS WARNERI;

EID: 49049084197     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.06.022     Document Type: Article

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