Pilot study to investigate the possibility of cytogenetic and physiological changes in bio-electrosprayed human lymphocyte cells

Regenerative Medicine

Volumn 3, Issue 3, 2008, Pages 343-349

  Kempski, Helena ^a   Austin, Nicola ^a   Roe, Amy ^a   Chatters, Steve ^a   Jayasinghe, Suwan N ^b  

^a GREAT ORMOND STREET HOSPITAL   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Aerodynamically assisted bio jetting threading; Bio electrosprays; Cell electrospinning; Human blood cells; Karyotyping

Indexed keywords

ARTICLE; CELL CULTURE; CELL POPULATION; CELL STRUCTURE; CELL VIABILITY; CHROMOSOME ABERRATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOGENETICS; ELECTROPHORESIS; ELECTROSPRAY; FEASIBILITY STUDY; FLOW CYTOMETRY; FLUORESCENCE; GENE LOCUS; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; KARYOTYPE; LYMPHOCYTE; LYMPHOCYTE FUNCTION; MAMMAL CELL; MICROARRAY ANALYSIS; PILOT STUDY; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM; TISSUE ENGINEERING; ANIMAL; BIOLOGICAL MODEL; COHORT ANALYSIS; CYTOLOGY; DNA MICROARRAY; ELECTROCHEMISTRY; FLUORESCENCE IN SITU HYBRIDIZATION; KARYOTYPING; METABOLISM; METHODOLOGY; MONONUCLEAR CELL; REGENERATIVE MEDICINE;

ANIMALS; CHROMOSOME ABERRATIONS; COHORT STUDIES; CYTOGENETICS; ELECTROCHEMISTRY; FLOW CYTOMETRY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; KARYOTYPING; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTES; MODELS, BIOLOGICAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PILOT PROJECTS; REGENERATIVE MEDICINE;

EID: 48849098482     PISSN: 17460751     EISSN: None     Source Type: Journal    
DOI: 10.2217/17460751.3.3.343     Document Type: Article

References (22)

1. Ringe J, Kaps C, Burmester GR, Sittinger M: Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften 89, 338-351 (2002).
2. Hipp J, Atala A: Tissue engineering, stem cells, cloning, and parthenogenesis: new paradigms for therapy. J. Exp. Clin. Assist. Reprod. 1, 3 (2004).
3. Ibrahim SF, van den Engh G: Flow cytometry and cell sorting.
4. Adv Biochem. Engin./Biotechnol. 106, 19-39 (2008).
5. Boland T, Xu T, Damon B, Cui X: Application of inkjet printing to tissue engineering. Biotechnol. J. 1, 910-917 (2006).
6. Roberts A, Wyslouzil BE, Bonassar L: Aerosol delivery of mammalian cells for tissue engineering. Biotechnol. Bioeng. 91, 801-807 (2005).
7. Jayasinghe SN, Qureshi AN, Eagles PAM: Electrohydrodynamic jet processing: An advanced electric-field-driven jetting phenomenon for processing living cells. Small 2, 216-219 (2006).
8. Jayasinghe SN, Eagles PAM, Qureshi AN: Electric field driven jetting: an emerging approach for processing living cells. Biotechnol. J. 1, 86-94 (2006).
9. Arumuganathar S, Irvine S, McEwan JR, Jayasinghe SN: Aerodynamically assisted bio-jets: the development of a novel and direct non-electric field driven methodology for engineering living organisms. Biomedical Materials: Materials for Tissue Engineering &, Regenerative Medicine 2, 158-168 (2007).
10. Townsend-Nicholson A, Jayasinghe SN: Cell Electrospinning: a unique biotechnique for encapsulating living organisms for generating active biological microthreads/scaffolds. Biomacromolecules 7, 3364-3369 (2006).
11. Jayasinghe SN, Irvine S, McEwan JR: Cell electrospinning highly concentrated cellular suspensions containing primary living organisms into cell-bearing threads and scaffolds. Nanomedicine 2, 555-567 (2007).
12. Irvine S, Arumuganathar S, McEwan JR, Jayasinghe SN: Coaxial aerodynamically assisted bio-jets: a versatile paradigm for directly engineering living primary organisms, Eng. Life, Sci. 7, 599-610 (2007).
13. Arumuganathar S, Irvine S, McEwan JR, Jayasinghe SN: A novel direct aerodynamically assisted threading methodology for generating biologically viable microthreads encapsulating living primary cells. J. Appl. Polymer Sci. 107, 1215-1225 (2008).
14. Rayleigh Lord: On the instability of a cylinder of viscous liquid under capillary force. Philos. Mag. 34, 145-156 (1892).
15. Zeleny J: The electric discharge from liquid points and hydrostatic method of measuring the electric intensity at their surface. Phys. Rev. 3, 69-91 (1914).
16. Taylor GI: Disintegration of water drops in an electric field. Proc. Royal Society A 280, 383-397 (1964).
17. Odenwälder PK, Irvine S, McEwan JR, Jayasinghe SN: Bio-electrosprays: A precision "drop and place" paradigm for safe handling and deployment of primary living organisms. Biotechnol. J. 2, 622-630 (2007).
18. Barry SP, Jayasinghe SN, Latchman DS, Stephanou A: Bio-electrospraying primary neonatal cardiac myocytes. Bioprocessing J. 6, 8-14 (2007).
19. Jayasinghe SN, Townsend-Nicholson A: Stable electric-field driven cone-jetting of concentrated biosuspensions. Lab. Chip 6, 1086-1090 (2006).
20. Jayasinghe SN, Townsend-Nicholson A: Bio-electrosprays: the next generation of electrified jet. Biotechnol. J. 1, 1018-1022 (2006).
21. ISCN: Guidelines for cancer cytogenetics. In: Supplement to an International System for Hunmn Cytogenetic Nomenclature. Mitelman F (Ed.). Karger, Basel, Switzerland (1995).
22. Jayasinghe SN: Advanced jet-protocols for directly engineering living cells: a genesis to alternative bio-handling. approaches for the life sciences. Regen. Med. 3, 49-61 (2008).