Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein

Veterinary Microbiology

Volumn 131, Issue 1-2, 2008, Pages 73-81

  Sun, Dongbo ^a   Feng, Li ^a,b   Shi, Hongyan ^a   Chen, Jianfei ^a   Cui, Xiaochen ^a   Chen, Hongyan ^a   Liu, Shengwang ^a   Tong, Youen ^a   Wang, Yunfeng ^a   Tong, Guangzhi ^a  

^a Harbin Veterinary Research Institute   (China)

^b HEILONGJIANG ACADEMY OF AGRICULTURAL SCIENCES   (China)

Author keywords

Epitope; Monoclonal antibody; PEDV; Pepscan; Phage display; Spike protein

Indexed keywords

MONOCLONAL ANTIBODY; SPIKE PROTEIN S1D; SPIKE PROTEIN S5D; SPIKE PROTEIN S6D; VIRUS FUSION PROTEIN; VIRUS PROTEIN;

ARTICLE; B LYMPHOCYTE; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; ESCHERICHIA COLI; GENETIC CODE; NONHUMAN; PORCINE EPIDEMIC DIARRHEA VIRUS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EPITOPES, B-LYMPHOCYTE; GASTROENTERITIS, TRANSMISSIBLE, OF SWINE; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; PORCINE EPIDEMIC DIARRHEA VIRUS; SWINE; VIRAL ENVELOPE PROTEINS;

ESCHERICHIA COLI; PORCINE EPIDEMIC DIARRHEA VIRUS;

EID: 48849096769     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2008.02.022     Document Type: Article

References (26)

1. Brian D.A., and Baric R.S. Coronavirus genome structure and replication. In: Enjuanes L. (Ed). Coronavirus Replication and Reverse Genetics (2005), Springer-Verlag, Berlin 2-22
2. Carvajal A., Lanza I., Diego R., Rubio P., and Girrnenes P. Seroprevalence of porcine epidemic diarrhea virus infection among different types of breeding swine farms in Spain. Prev. Vet. Med. 23 (1995) 33-40
3. Chae C., Kim O., Choi C., Min K., Cho W.S., Kim J., and Tai J.H. Prevalence of porcine epidemic diarrhea virus and transmissible gastroenteritis virus infection in Korean pigs. Vet. Rec. 147 (2000) 606-608
4. Chang S.H., Bae J.L., Kang T.J., Kim J., Chung G.H., Lim C.W., Laude H., Yang M.S., and Jang Y.S. Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus. Mol. Cells 14 (2002) 295-299
5. Chasey D., and Cartwright S.F. Virus-like particles associated with porcine epidemic diarrhea. Res. Vet. Sci. 25 (1978) 255-256
6. Cruz D.J., Kim C.J., and Shin H.J. Phage-displayed peptides having antigenic similarities with porcine epidemic diarrhea virus (PEDV) neutralizing epitopes. Virology 354 (2006) 28-34
7. De Haan C.A., Te Lintelo E., Raaben M., Wurdinger T., Bosch B.J., and Rottier P.J. Cooperative involvement of the S1 and S2 subunits of the murine coronavirus spike protein in receptor binding and extended host range. J. Virol. 80 (2006) 10909-10918
8. Duarte M., and Laude H. Sequence of the spike protein of the porcine epidemic diarrhea virus. J. Gen. Virol. 75 (1994) 1195-1200
9. Egberink H.F., Ederveen J., Callebaut P., and Horzinek M.C. Characterization of the structural proteins of porcine epizootic diarrhea virus, strain CV777. Am. J. Vet. Res. 49 (1988) 1320-1324
10. Fan J.H., and Li Y.J. Cloning and sequence analysis of the M gene of porcine epidemic diarrhea virus LJB/03. Virus Genes 30 (2005) 69-73
11. Follis K.E., York J., and Nunberg J.H. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology 350 (2006) 358-369
12. Holmes K.V. Coronaviruses. In: Knipe D.M., Howley P.M., and Griffin D.E. (Eds). Fields Virology (2001), Lippincott Williams & Wilkins, Philadelphia 1187-1197
13. Hofmann M., and Wyler R. Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV). Vet. Microbiol. 20 (1989) 131-142
14. Hua R.H., Zhou Y.J., Wang Y.F., Hua Y.Z., and Tong G.Z. Identification of two antigenic epitopes on SARS-CoV spike protein. Biochem. Biophys. Res. Commun. 319 (2004) 929-935
15. Kang T.J., Han S.C., Yang M.S., and Jang Y.S. Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants. Protein Expr. Purif. 46 (2006) 16-22
16. Kweon C.H., Kwon B.J., Jung T.S., Kee Y.J., Hur D.H., Hwang E.K., Rhee J.C., and An S.H. Isolation of porcine epidemic diarrhea virus (PEDV) in Korea. Korean J. Vet. Res. 33 (1993) 249-254
17. Li B.X., Ge J.W., and Li Y.J. Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus. Virology 365 (2007) 166-172
18. Ma G.P., Feng Y.J., Gao F., Wang J.Z., Liu C., and Li Y.J. Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core. Biochem. Biophys. Res. Commun. 337 (2005) 1301-1307
19. Park S.J., Song D.S., Ha G.W., and Park B.K. Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13. Virus Genes 35 (2006) 55-64
20. Pensaert M.B., and Debouck P. A new coronavirus-like particle associated with diarrhea in swine. Arch. Virol. 58 (1978) 243-247
21. Sun D.B., Feng L., Shi H.Y., Chen J.F., Liu S.W., Chen H.Y., and Wang Y.F. Spike protein region (aa 636-789) of porcine epidemic diarrhea virus is essential for induction of neutralizing antibodies. Acta Virol. 51 (2007) 327-338
22. Sun D.B., Lang H.W., Shi H.Y., Chen J.F., Cui X.C., Wang C.B., Tong Y.E., and Feng L. Screening and identification of B cell epitopes of porcine epidemic diarrhea virus spike glycoprotein by phage display. Prog. Biochem. Biophys. 34 (2007) 971-977
23. Takahashi K., Okada K., and Ohshima K. An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan. Jpn. J. Vet. Sci. 45 (1983) 829-832
24. Timoney J.F., Gillespie J.H., Scott F.W., and Barlough J.E. Hagan and Bruner's Microbiology and Infectious Diseases of Domestic Animals (1998), Comstock Publishing Associates, Ithaca pp. 897-898
25. Wu X.D., Shang B., Yang R.F., Yu H., Ma Z.H., Shen X., Ji Y.Y., Lin Y., Wu Y.D., Lin G.M., Tian L., Gan X.Q., Yang S., Jiang W.H., Dai E.H., Wang X.Y., Jiang H.L., Xie Y.H., Zhu X.L., Pei G., Li L., Wu J.R., and Sun B. The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells. Cell Res. 14 (2004) 400-406
26. Yeo S.G., Hernandez M., Krell P.J., and Nagy E.E. Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99. Virus Genes 26 (2003) 239-246