In vivo analysis of Aspergillus fumigatus developmental gene expression determined by real-time reverse transcription-PCR

Infection and Immunity

Volumn 76, Issue 8, 2008, Pages 3632-3639

  Gravelat, Fabrice N ^a   Doedt, Thomas ^b   Chiang, Lisa Y ^b   Liu, Hong ^b   Filler, Scott G ^b,c   Patterson, Thomas F ^d   Sheppard, Donald C ^a  

^a MCGILL UNIVERSITY   (Canada)

^b HARBOR UCLA MEDICAL CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; MYCOTOXIN; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASPERGILLUS FUMIGATUS; CONIDIUM; FUNGAL DEVELOPMENT; FUNGUS HYPHAE; GENE EXPRESSION; IN VITRO STUDY; IN VIVO STUDY; LUNG ASPERGILLOSIS; MALE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; ASPERGILLOSIS; BAGG ALBINO MOUSE; BIOSYNTHESIS; FUNGUS SPORE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; LUNG; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY;

ANIMALS; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, FUNGAL; HYPHAE; LUNG; MALE; MICE; MICE, INBRED BALB C; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPORES, FUNGAL; VIRULENCE FACTORS;

EID: 48849094142     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.01483-07     Document Type: Article

References (25)

1. Adams, T. H., W. A. Hide, L. N. Yager, and B. N. Lee. 1992. Isolation of a gene required for programmed initiation of development by Aspergillus nidulans. Mol. Cell. Biol. 12:3827-3833.
2. Arruda, L. K., T. A. Platts-Mills, J. W. Fox, and M. D. Chapman. 1990. Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins. J. Exp. Med. 172:1529-1532.
3. Axelrod, D. E., M. Gealt, and M. Pastushok. 1973. Gene control of developmental competence in Aspergillus nidulans. Dev. Biol. 34:9-15.
4. Belkacemi, L., R. C. Barton, V. Hopwood, and E. G. Evans. 1999. Determination of optimum growth conditions for gliotoxin production by Aspergillus fumigatus and development of a novel method for gliotoxin detection. Med. Mycol. 37:227-233.
5. Bok, J. W., D. Chung, S. A. Balajee, K. A. Marr, D. Andes, K. F. Nielsen, J. C. Frisvad, K. A. Kirby, and N. P. Keller. 2006. GliZ, a transcriptional regulator of gliotoxin biosynthesis, contributes to Aspergillus fumigatus virulence. Infect. Immun. 74:6761-6768.
6. Bowman, J. C., G. K. Abruzzo, J. W. Anderson, A. M. Flattery, C. J. Gill, V. B. Pikounis, D. M. Schmatz, P. A. Liberator, and C. M. Douglas. 2001. Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate. Antimicrob. Agents Chemother. 45:3474-3481.
7. Brakhage, A. A., and K. Langfelder. 2002. Menacing mold: the molecular biology of Aspergillus fumigatus. Annu. Rev. Microbiol. 56:433-455.
8. Coyle, C. M., and D. G. Panaccione. 2005. An ergot alkaloid biosynthesis gene and clustered hypothetical genes from Aspergillus fumigatus. Appl. Environ. Microbiol. 71:3112-3118.
9. Cramer, R. A., Jr., M. P. Gamcsik, R. M. Brooking, L. K. Najvar, W. R. Kirkpatrick, T. F. Patterson, C. J. Balibar, J. R. Graybill, J. R. Perfect, S. N. Abraham, and W. J. Steinbach. 2006. Disruption of a nonribosomal peptide synthetase in Aspergillus fumigatus eliminates gliotoxin production. Eukaryot. Cell 5:972-980.
10. Denning, D. W. 1996. Therapeutic outcome in invasive aspergillosis. Clin. Infect. Dis. 23:608-615.
11. Ebina, K., H. Sakagami, K. Yokota, and H. Kondo. 1994. Cloning and nucleotide sequence of cDNA encoding Asp-hemolysin from Aspergillus fumigatus. Biochim. Biophys. Acta 1219:148-150.
12. Fraser, R. S. 1993. Pulmonary aspergillosis: pathologic and pathogenetic features. Pathol. Annu. 28:231-277.
13. Gardiner, D. M., A. J. Cozijnsen, L. M. Wilson, M. S. Pedras, and B. J. Howlett. 2004. The sirodesmin biosynthetic gene cluster of the plant pathogenic fungus Leptosphaeria maculans. Mol. Microbiol. 53:1307-1318.
14. Herbrecht, R., D. W. Denning, T. F. Patterson, J. E. Bennett, R. E. Greene, J. W. Oestmann, W. V. Kern, K. A. Marr, P. Ribaud, O. Lortholary, R. Sylvester, R. H. Rubin, J. R. Wingard, P. Stark, C. Durand, D. Caillot, E. Thiel, P. H. Chandrasekar, M. R. Hodges, H. T. Schlamm, P. F. Troke, and B. de Pauw. 2002. Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. N. Engl. J. Med. 347:408-415.
15. Kosalec, I., S. Pepeljnjak, and M. Jandrlic. 2005. Influence of media and temperature on gliotoxin production in Aspergillus fumigatus strains. Arh. Hig. Rada Toksikol. 56:269-273.
16. Lamy, B., and J. Davies. 1991. Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide. Nucleic Acids Res. 19:1001-1006.
17. Large, J. P. 1999. Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12:310-350.
18. Livak, K. J., and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402-108.
19. Monroy, F., and D. C. Sheppard. 2005. Taf1: a class II transposon of Aspergillus fumigatus. Fungal Genet. Biol. 42:638-645.
20. Pfafll, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45.
21. Sheppard, D. C., T. Doedt, L. Y. Chiang, H. S. Kim, D. Chen, W. C. Nierman, and S. G. Filler. 2005. The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence. Mol. Biol. Cell 16:5866-5879.
22. Sheppard, D. C., G. Rieg, L. Y. Chiang, S. G. Filler, J. E. Edwards, Jr., and A. S. Ibrahim. 2004. Novel inhalational murine model of invasive pulmonary aspergillosis. Antimicrob. Agents Chemother. 48:1908-1911.
23. Spikes, S., R. Xu, C. K. Nguyen, G. Chamilos, D. P. Kontoyiannis, R. H. Jacobson, D. E. Ejzykowicz, L. Y. Chiang, S. G. Filler, and G. S. May. 2008. Gliotoxin production in Aspergillus fumigatus contributes to host-specific differences in virulence. J. Infect. Dis. 197:479-486.
24. Sugui, J. A., J. Pardo, Y. C. Chang, K. A. Zarember, G. Nardone, E. M. Galvez, A. Mullbacher, J. I. Gallin, M. M. Simon, and K. J. Kwon-Chung. 2007. Gliotoxin is a virulence factor of Aspergillus fumigatus: gliP deletion attenuates virulence in mice immunosuppressed with hydrocortisone. Eukaryot. Cell 6:1562-1569.
25. Wu, J., and B. L. Miller. 1997. Aspergillus asexual reproduction and sexual reproduction are differentially affected by transcriptional and translational mechanisms regulating stunted gene expression. Mol. Cell. Biol. 17:6191-6201.