Characterization of C4d immunostaining utilizing paraffin-embedded tissue of nonpresensitized pediatric heart transplant patients

Pediatric and Developmental Pathology

Volumn 11, Issue 3, 2008, Pages 181-184

  Galambos, Csaba ^a   Feingold, Brian ^a   Webber, Steven A ^a  

^a CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

Author keywords

Alloantibody; Complement C4d; Heart biopsy; Humoral rejection; Paraffin embedded tissue; Pediatric heart transplantation

Indexed keywords

COMPLEMENT COMPONENT C4D; PARAFFIN;

ACUTE GRAFT REJECTION; ARTICLE; BLOOD SAMPLING; CAPILLARY; CARDIAC GRAFT REJECTION; CHILD; CHRONIC GRAFT REJECTION; CONTROLLED STUDY; GRAFT RECIPIENT; HEART MUSCLE BIOPSY; HEART TRANSPLANTATION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; HUMORAL IMMUNITY; IMMUNOHISTOCHEMISTRY; ISCHEMIA; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RANDOMIZATION; REPERFUSION INJURY; SENSITIZATION; SEROLOGY;

CHILD; CHILD, PRESCHOOL; COMPLEMENT C4B; ENDOCARDIUM; GRAFT REJECTION; HEART TRANSPLANTATION; HUMANS; IMMUNOHISTOCHEMISTRY; INFANT; PARAFFIN EMBEDDING; PEPTIDE FRAGMENTS;

EID: 48849085456     PISSN: 10935266     EISSN: 16155742     Source Type: Journal    
DOI: 10.2350/07-04-0259.1     Document Type: Article

References (21)

1. Feucht HE, Opelz G. The humoral immune response towards HLA class II determinants in renal transplantation. Kidney Int 1996;50: 1464-1475.
2. Baldwin WM III, Kasper EK, Zachary AA, Wasowska BA, Rodriguez ER. Beyond C4d: other complement-related diagnostic approaches to antibody-mediated rejection. Am J Transplant 2004;4:311-318.
3. Bohmig GA, Exner M, Habicht A, et al. Capillary C4d deposition in kidney allografts: a specific marker of alloantibody-dependent graft injury. J Am Soc Nephrol 2002;13:1091-1099.
4. Behr TM, Feucht HE, Richter K, et al. Detection of humoral rejection in human cardiac allografts by assessing the capillary deposition of complement fragment C4d in endomyocardial biopsies. J Heart Lung Transplant 1999;18:904-912.
5. Ionescu DN, Girnita AL, Zeevi A, et al. C4d deposition in lung allografts is associated with circulating anti-HLA alloantibody. Transpl Immunol 2005;15:63-68.
6. Fine RN. Pediatric Solid Organ Transplantation, 2nd ed. Maiden, MA: Blackwell Publishing, 2007;383.
7. Haga H, Egawa H, Fujimoto Y, et al. Acute humoral rejection and C4d immunostaining in ABO blood type-incompatible liver transplantation. Liver Transpl 2006;12:457-464.
8. Chantranuwat C, Qiao JH, Kobashigawa J, et al. Immunoperoxidase staining for C4d on paraffin-embedded tissue in cardiac allograft endomyocardial biopsies: comparison to frozen tissue immunofluorescence. Appl Immunohistochem Mol Morphol 2004;12:166-171.
9. Hammond ME, Stehlik J, Snow G, et al. Utility of histologic parameters in screening for antibody-mediated rejection of the cardiac allograft: a study of 3,170 biopsies. J Heart Lung Transplant 2005;24: 2015-2021.
10. Hammond EH, Yowell RL, Nunoda S, et al. Vascular (humoral) rejection in heart transplantation: pathologic observations and clinical implications. J Heart Transplant 1989;8:430-443.
11. Casarez TW, Perens G, Williams RJ, et al. Humoral rejection in pediatric orthotopic heart transplantation. J Heart Lung Transplant 2007;26:114-119.
12. Smith RN, Brousaides N, Grazette L, et al. C4d deposition in cardiac allografts correlates with alloantibody. J Heart Lung Transplant 2005; 24:1202-1210.
13. Lones MA, Czer LS, Trente A, et al. Clinical-pathologic features of humoral rejection in cardiac allografts: a study in 81 consecutive patients. J Heart Lung Transplant 1995;14:151-162.
14. Gebel HM, Bray RA. Sensitization and sensitivity: defining the unsensitized patient. Transplantation 2000;69:1370-1374.
15. Tambur AR, Bray RA, Takemoto SK, et al. Flow cytometric detection of HLA-specific antibodies as a predictor of heart allograft rejection. Transplantation 2000;70:1055-1059.
16. McKenna RM, Takemoto SK, Terasaki PI. Anti-HLA antibodies after solid organ transplantation. Transplantation 2000;69:319-326.
17. Ronco P, Debiec H, Guigonis V Mechanisms of disease: alloimmunization in renal diseases. Nat Clin Pract 2006;2:388-397.
18. Baldwin WM III, Samaniego-Picota M, Kasper EK, et al. Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes. Transplantation 1999;68:894-900.
19. Crespo-Leiro MG, Veiga-Barreiro A, Domenech N, et al. Humoral heart rejection (severe allograft dysfunction with no signs of cellular rejection or ischemia): incidence, management, and the value of C4d for diagnosis. Am J Transplant 2005;5:2560-2564.
20. Haas M, Ratner LE, Montgomery RA. C4d staining of perioperative renal transplant biopsies. Transplantation 2002;74:711-717.
21. Stewart S, Winters GL, Fishbein MC, et al. Revision of the 1990 working formulation for the standardization of nomenclature in the diagnosis of heart rejection. J Heart Lung Transplant 2005;24:1710-1720.