-
1
-
-
4544327894
-
-
Zha S., Yegnasubramanian V., Nelson W.G., Isaacs W.B., and De Marzo A.M. Cancer Lett. 215 (2004) 1
-
(2004)
Cancer Lett.
, vol.215
, pp. 1
-
-
Zha, S.1
Yegnasubramanian, V.2
Nelson, W.G.3
Isaacs, W.B.4
De Marzo, A.M.5
-
7
-
-
14944365641
-
-
Lazzarato L., Rolando B., Lolli M.L., Tron G.C., Fruttero R., Gasco A., Deleide G., and Guenther H.L. J. Med. Chem. 48 (2005) 1322
-
(2005)
J. Med. Chem.
, vol.48
, pp. 1322
-
-
Lazzarato, L.1
Rolando, B.2
Lolli, M.L.3
Tron, G.C.4
Fruttero, R.5
Gasco, A.6
Deleide, G.7
Guenther, H.L.8
-
8
-
-
48749128900
-
-
-4 M as solubility allows). Then, 40 μM of calcium ionophore A 23187 alone was added, and incubation was carried out at 37 °C for 20 h to stimulate the COX-1 pathway. For the COX-2 pathway, samples stimulated with A 23187+LPS (500 μg) were incubated for 20 h. Eicosanoids were extracted from the samples into ethyl acetate. Samples were then evaporated to dryness under nitrogen, re-suspended in the mobile phase to precipitate proteins and then centrifuged.
-
-4 M as solubility allows). Then, 40 μM of calcium ionophore A 23187 alone was added, and incubation was carried out at 37 °C for 20 h to stimulate the COX-1 pathway. For the COX-2 pathway, samples stimulated with A 23187+LPS (500 μg) were incubated for 20 h. Eicosanoids were extracted from the samples into ethyl acetate. Samples were then evaporated to dryness under nitrogen, re-suspended in the mobile phase to precipitate proteins and then centrifuged. The supernatant was directly analyzed by HPLC using Hypersil ODS 3 μM columns (12.5 mm × 0.46 mm), a methanol/water/acetic acid (80:20:0.08) mobile phase at a flow rate of 1 mL/min and UV detection firstly at 270 nm and 12 min later at 245 nm.
-
-
-
-
10
-
-
48749103522
-
-
4 cells/well) on 24-well plates. After 3 days, the cell medium was changed to serum free medium, and the cells were starved for 24 h for culture synchronization. Cells were then incubated in culture medium that contained various concentrations of test compounds (1; 10; 50 and 100 μM as solubility allows), each dissolved in less than 0.1% DMSO. After incubating for a given amount of time (usually 72 h), cell growth was estimated by the colorimetric MTT test.
-
4 cells/well) on 24-well plates. After 3 days, the cell medium was changed to serum free medium, and the cells were starved for 24 h for culture synchronization. Cells were then incubated in culture medium that contained various concentrations of test compounds (1; 10; 50 and 100 μM as solubility allows), each dissolved in less than 0.1% DMSO. After incubating for a given amount of time (usually 72 h), cell growth was estimated by the colorimetric MTT test.
-
-
-
-
11
-
-
48749108746
-
-
EPR spectra were recorded using a Bruker ELEXYS E580 spectrometer operating at 9.5 GHz with a 100 kHz high frequency modulation and a modulation amplitude of 1 G. The sample solutions were examined in quartz cell inserted in standard thermoregulated cavity for the EPR spectra at liquid nitrogen temperature. The EPR signal of NO° trapped by iron (II) N,N-diethyldithiocarbamate consists in three lines with a nitrogen hyperfine splitting constant of 13 G. The concentration of NO° released was quantified by double integration of EPR signal using 2,2,6,6-tetramethyl-1-piperidinyloxy TEMPO as standard.
-
EPR spectra were recorded using a Bruker ELEXYS E580 spectrometer operating at 9.5 GHz with a 100 kHz high frequency modulation and a modulation amplitude of 1 G. The sample solutions were examined in quartz cell inserted in standard thermoregulated cavity for the EPR spectra at liquid nitrogen temperature. The EPR signal of NO° trapped by iron (II) N,N-diethyldithiocarbamate consists in three lines with a nitrogen hyperfine splitting constant of 13 G. The concentration of NO° released was quantified by double integration of EPR signal using 2,2,6,6-tetramethyl-1-piperidinyloxy TEMPO as standard.
-
-
-
|
