Enzymatic deacetylation of chitin by extracellular chitin deacetylase from a newly screened Mortierella sp. DY-52

Journal of Microbiology and Biotechnology

Volumn 18, Issue 4, 2008, Pages 759-766

  Kim, Young Ju ^a   Zhao, Yong ^a   Oh, Kyung Taek ^a   Nguyen, Van Nam ^a   Park, Ro Dong ^a  

^a Chonnam National University   (South Korea)

Author keywords

Chitin deacetylase; DY 52 ; Mortierella sp.; N acetylchitooligomers; Water solube chitin

Indexed keywords

CALCIUM; CHITIN; CHITIN DEACETYLASE; CHITOSAN; COBALT; ENZYME; GLUCOSE; N ACETYLGLUCOSAMINE; OLIGOMER; RIBOSOME RNA; AMIDASE; FUNGAL DNA; FUNGAL PROTEIN; RIBOSOME DNA; RNA 18S;

ACIDITY; ARTICLE; CONTROLLED STUDY; DEACETYLATION; ENZYME ACTIVITY; ENZYME ASSAY; FUNGUS GROWTH; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; LIQUID CULTURE; MOLECULAR WEIGHT; MORTIERELLA; MUCOR; MUCOR ROUXII; NONHUMAN; NUCLEOTIDE SEQUENCE; THERMOSTABILITY; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TRANSPORT; TEMPERATURE;

DECAPODA (CRUSTACEA); FUNGI; MORTIERELLA;

AMIDOHYDROLASES; CHITIN; CULTURE MEDIA; DNA, FUNGAL; DNA, RIBOSOMAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; FUNGAL PROTEINS; MORTIERELLA; PROTEIN TRANSPORT; RNA, RIBOSOMAL, 18S; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 48649105376     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Alfonso, C., O. M. Nuero, F. Santamaria, and F. Reyes. 1995. Purification of a heat-stable chitin deacetylase from Aspergillus nidulans and its role in cell wall degradation. Curr. Microbiol. 30: 49-54.
2. Araki, Y. and E. Ito. 1975. A pathway of chitosan formation in Mucor rouxii. Eur. J. Biochem. 55: 71-78.
3. Aruchami, M., N. Gowri, and G. Sundara-Rajulu. 1986. Chitin deacetylases in invertebrates, pp. 263-265. In R. A. A. Muzzarelli, C. Jeuniaux, and G. W. Gooday (eds.), Chitin in Nature and Technology. Plenum Press, New York, U.S.A.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Cai, J., J. H. Yang, Y. M. Du, L. H. Fan, Y. L. Qiu, J. Li, and J. F. Kennedy. 2006. Purification and characterization of chitin deacetylase from Scopulariopsis brevicaulis. Carbohydr. Polym. 1-7.
6. Chang, K. L., G. Tsai, J. Lee, and W. R. Fu. 1997. Heterogeneous N-deacetylation of chitin in alkaline solution. Carbohydr. Res. 303: 327-332.
7. Gao, X., T. Katsumoto, and K. Onoder. 1995. Purification and characterization of chitin deacetylase from Absidia coerulea. J. Biochem. 117: 257-263.
8. Hekmat, O., K. Tokuyasu, and S. G. Withers. 2003. Subsite structure of the endo-type chitin deacetylase from Deuteromycete, Colletotrichum lindemuthianum: An investigation using study-state kinetic analysis and MS. Biochem. J. 374: 369-380.
9. Imoto, T. and K. Yagishita. 1971. A simple activity measurement of lysozyme. Agric. Biol. Chem. 35: 1154-1156.
10. Jeuniaux, C. 1982. La chitine dans le regne animal. Bull. Soc. Zool. Fr. 107: 363-386.
11. Kadokura, K., A. Rokutani, M. Yamamoto, T. Ikegami, H. Sugita, S. Itoi, W. Hakamata, T. Oku, and T. Nishio. 2006. Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. Appl. Microbiol. Biotechnol. 75: 357-365.
12. Kafetzopoulos, D., A. Martinou, and V. Bouriotis. 1993. Bioconversion of chitin to chitosan: Purification and characterization of chitin deacetylase from Mucor rouxii. Proc. Natl. Acad. Sci. USA 90: 2564-2568.
13. Kauss, H., W. Jeblick, and D. H. Young. 1983. Chitin deacetylase from the plant pathogen Colletotrichum lindemuthianum. Plant Sci. Lett. 28: 231-236.
14. Kuk, J. H., W. J. Jung, K. Y. Kim, and R. D. Park. 2005. Enzymatic characteristics and applications of microbial chitin deacetylase. Kor. J. Microbiol. Biotechnol. 33: 9-15.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
16. Martinou, A., D. Koutsioulis, and V. Bouritiotis. 2002. Expression, purification, and characterization of a cobalt-activated chitin deacetylase (Cda2p) from Saccharomyces cerevisiae. Prot. Expr. Purif. 24: 111-116.
17. Martinou, A., I. Tsigos, and V. Bouriotis. 1997. Preparation of chitosan by enzymatic deacetylation, pp. 501-505. In R. A. A. Muzzarelli and M. G. Peter (eds.), Chitin Handbook. Eur. Chitin Soc., Atec, Grottammare, Italy.
18. Nahar, P., V. Ghormade, and V. Deshpande. 2004. The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: Possible edge to entomopathogenic fungi in the biological control of insect pests. J. Invertebr. Pathol. 85: 80-88.
19. Ruiz-Herrera, J. 1978. The distribution and quantitative importance of chitin in fungi, pp. 11-21. In R. A. A. Muzzarelli and E. R. Pariser (eds.), Proceedings of the 1st International Conference on Chitin and Chitosan, Massachusetts Institute of Technology, Cambridge.
20. Sannan, T., K. Kurita, and Y. Iwakura. 1975. Studies on chitin. 1. Solubility change by alkaline treatment and film casting. Makromol. Chem. 176: 1191-1195.
21. Shin, W. S., J. C. Kil, and G. M. Park. 2006. Antiprotozoal activity of deacetylated chitosan oligosaccharide (dp 2-8) on Trichomonas vaginalis. J. Microbiol. Biotechnol. 16: 1984-1989.
22. Srinivasan, V. R. 1998. Biotransformation of chitin to chitosan. US Patent 5739015.
23. Stevens, W. F., N. N. Win, C. H. Ng, C. Pichyangkura, and S. Chandrkrachang. 1997. Towards technical biocatalytic deacetylation of chitin, pp. 40-47. In A. Domard, G. A. F. Roberts, and K. M. Varum (eds.), Advances in Chitin Science, Vol. 2. Jacques Andre, Lyon.
24. Toei, K. and T. Kokara. 1976. A conductometric method for colloid titrations. Anal. Chem. Acta 83: 59-65.
25. Tokuyasu, K., M. O. Kameyama, and K. Hayashi. 1996. Purification and characterization of extracellular chitin deacetylase from Colletotrichum lindemuthianum. Biosci. Biotech. Biochem. 60: 1598-1603.
26. Trudel, J. and A. Asselain. 1990. Detection of chitin deacetylase activity after polyacrylamide gel electrophoresis. Anal. Biochem. 189: 249-253.
27. Tsigos, I. and V. Bouriotis. 1995. Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum. J. Biol. Chem. 270: 26286-26291.
28. Win, N. N. and W. F. Stevens. 2001. Shrimp chitin as substrate for fungal chitin deacetylase. Appl. Microbiol. Biotechnol. 57: 334-341.
29. White, T. J., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. Innis, D. Gelfand, J. Sninsky, and T. White (eds.), A Guide to Methods and Applications. Academic Press, San Diego, U.S.A.