Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: Implications for tropoelastin microassembly

Biomacromolecules

Volumn 9, Issue 7, 2008, Pages 1739-1744

  Tu, Yidong ^a   Weiss, Anthony S ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AGGLOMERATION; BIODEGRADABLE POLYMERS; BIOLOGICAL SYSTEMS; CELL MEMBRANES; CONCENTRATION (PROCESS); DROPS; FLUID MECHANICS; FUSION REACTIONS; NUCLEAR PHYSICS; POLYSACCHARIDES; SULFATE MINERALS; SULFUR COMPOUNDS;

AGGREGATE FORMATION; CELL SURFACES; CELLULAR PRODUCTION; CHONDROITIN SULFATE; CRITICAL CONCENTRATIONS; DROPLET BEHAVIORS; DROPLET FORMATION; ELASTOGENESIS; EXTRA CELLULAR MATRICES; GLYCOSAMINOGLYCAN; GLYCOSAMINOGLYCANS; HEPARAN SULFATE; HIGHER CONCENTRATIONS; LOW CONCENTRATIONS; MICRO ASSEMBLIES; TROPOELASTIN;

DROP FORMATION;

DERMATAN SULFATE; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; HEPARIN; TROPOELASTIN;

ARTICLE; CELL SURFACE; COACERVATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; MICROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN IMMOBILIZATION; PROTEIN SECRETION; TEMPERATURE;

ADHESIONS; DERMATAN SULFATE; ELASTIN; EXTRACELLULAR MATRIX; GLYCOSAMINOGLYCANS; HEPARIN; HEPARITIN SULFATE; MODELS, BIOLOGICAL; PROTEIN BINDING; SOLUTIONS; TROPOELASTIN;

SPHERULES;

EID: 48449091794     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm7013153     Document Type: Article

References (27)

1. Mithieux, S. M.; Weiss, A. S. Elastin. Adv. Protein Chem. 2005, 70, 437-61.
2. Vrhovski, B.; Weiss, A. S. Biochemistry of tropoelastin. Eur. J. Biochem. 1998, 258 (1), 1-18.
3. Pasquali-Ronchetti, I.; Baccarani-Contri, M. Elastic fiber during development and aging. Microsc. Res. Tech. 1997, 38 (4), 428-35.
4. Pasquali-Ronchetti, I.; Fornieri, C.; Baccarani-Contri, M.; Quaglino, D. Ultrastructure of elastin. Ciba Found Symp. 1995, 192, 31-42; discussion 42-50.
5. Keeley, F. W.; Bellingham, C. M.; Woodhouse, K. A. Elastin as a self-organizing biomaterial: Use of recombinantly expressed human elastin polypeptides as a model for investigations of structure and self-assembly of elastin. Philos. Trans. R. Soc.London, Ser. B 2002, 357 (1418), 185-9.
6. Urry, D. W. Entropic elastic processes in protein mechanisms. II. Simple (passive) and coupled (active) development of elastic forces. J. Protein Chem. 1988, 7 (2), 81-114.
7. Kagan, H. M.; Sullivan, K. A. Lysyl oxidase: Preparation and role in elastin biosynthesis. Methods Enzymol. 1982, 82, 637-50.
8. Kagan, H. M.; Cai, P. Isolation of active site peptides of lysyl oxidase. Methods Enzymol. 1995, 258, 122-32.
9. Kozel, B. A.; Rongish, B. J.; Czirok, A.; Zach, J.; Little, C. D.; Davis, E. C.; Knutsen, R. H.; Wagenseil, J. E.; Levy, M. A.; Mecham, R. P. Elastic fiber formation: A dynamic view of extracellular matrix assembly using timer reporters. J. Cell. Physiol. 2006, 207 (1), 87-96.
10. Urry, D. W.; Starcher, B.; Partridge, S. M. Coacervation of solubilized elastin effects a notable conformational change. Nature 1969, 222 (5195), 795-6.
11. Vrhovski, B.; Jensen, S.; Weiss, A. S. Coacervation characteristics of recombinant human tropoelastin. Eur. J. Biochem. 1997, 250 (1), 92-8.
12. Toonkool, P.; Jensen, S. A.; Maxwell, A. L.; Weiss, A. S. Hydrophobic domains of human tropoelastin interact in a context-dependent manner. J. Biol. Chem. 2001, 276 (48), 44575-80.
13. Cox, B. A.; Starcher, B. C.; Urry, D. W. Communication: Coacervation of tropoelastin results in fiber formation. J. Biol. Chem. 1974, 249 (3), 997-8.
14. Urry, D. W.; Long, M. M. On the conformation, coacervation and function of polymeric models of elastin. Adv. Exp. Med. Biol. 1977, 79, 685-714.
15. Clarke, A. W.; Wise, S. G.; Cain, S. A.; Kielty, C. M.; Weiss, A. S. Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin. Biochemistry 2005, 44 (30), 10271-81.
16. Clarke, A. W.; Arnspang, E. C.; Mithieux, S. M.; Korkmaz, E.; Braet, F.; Weiss, A. S. Tropoelastin massively associates during coacervation to form quantized protein spheres. Biochemistry 2006, 45 (33), 9989-96.
17. Toonkool, P.; Regan, D. G.; Kuchel, P. W.; Morris, M. B.; Weiss, A. S. Thermodynamic and hydrodynamic properties of human tropoelastin. Analytical ultracentrifuge and pulsed field-gradient spinecho NMR studies. J. Biol. Chem. 2001, 276 (30), 28042-50.
18. Broekelmann, T. J.; Kozel, B. A.; Ishibashi, H.; Werneck, C. C.; Keeley, F. W.; Zhang, L.; Mecham, R. P. Tropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain. J. Biol. Chem. 2005, 280 (49), 40939-47.
19. Cain, S. A.; Baldock, C.; Gallagher, J.; Morgan, A.; Bax, D. V.; Weiss, A. S.; Shuttleworth, C. A.; Kielty, C. M. Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly. J. Biol. Chem. 2005, 280 (34), 30526-37.
20. Gheduzzi, D.; Guerra, D.; Bochicchio, B.; Pepe, A.; Tamburro, A. M.; Quaglino, D.; Mithieux, S.; Weiss, A. S.; Pasquali Ronchetti, I. Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers. Matrix Biol. 2005, 24 (1), 15-25.
21. Wu, W. J.; Vrhovski, B.; Weiss, A. S. Glycosaminoglycans mediate the coacervation of human tropoelastin through dominant charge interactions involving lysine side chains. J. Biol. Chem. 1999, 274 (31), 21719-24.
22. Martin, S. L.; Vrhovski, B.; Weiss, A. S. Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin. Gene 1995, 154 (2), 159-66.
23. Keane, F. M.; Clarke, A. W.; Foster, T. J.; Weiss, A. S. The N-terminal A domain of Staphylococcus aureus fibronectin-binding protein A binds to tropoelastin. Biochemistry 2007, 46 (24), 7226-32.
24. Tu, Y.; Mithieux, S. M.; Clarke, A. W.; Weiss, A. S., Tropoelastin initially assembles through head-to-tail interactions as a prelude to coacervation, 2008, manuscript in preparation.
25. Varadarajan, R.; Sharma, D.; Chakraborty, K.; Patel, M.; Citron, M.; Sinha, P.; Yadav, R.; Rashid, U.; Kennedy, S.; Eckert, D.; Geleziunas, R.; Bramhill, D.; Schleif, W.; Liang, X.; Shiver, J. Characterization of gp120 and its single-chain derivatives, gp120-CD4D12 and gp120-M9: Implications for targeting the CD4i epitope in human immunodeficiency virus vaccine design. J. Virol. 2005, 79 (3), 1713-23.
26. Du, L.; Zhang, Z.; Luo, X.; Chen, K.; Shen, X.; Jiang, H. Binding investigation of human 5-lipoxygenase with its inhibitors by SPR technology correlating with molecular docking simulation. J. Biochem. (Tokyo) 2006, 139 (4), 715-23.
27. Gallagher, J. T.; Walker, A. Molecular distinctions between heparan sulphate and heparin: Analysis of sulphation patterns indicates heparan sulphate and heparin are separate families of N-sulphated polysaccharides. Biochem. J. 1985, 230 (3), 665-674.